دوره 5، شماره 2 - ( 6-1397 )                   جلد 5 شماره 2 صفحات 118-127 | برگشت به فهرست نسخه ها


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Hassanpour H, Shokrzadeh Lamuki M, Tabari R, Rezaee F, Rezaee F. The phytochemical study and antiprolifrative effect of hydroalcoholic extract of Adiantum capillus-veneris L. on MCF-7 and MRC-5 cell lines. nbr. 2018; 5 (2) :118-127
URL: http://nbr.khu.ac.ir/article-1-3059-fa.html
حسن پور حسانه، شکرزاده لموکی محمد، طبری رضا، رضایی فاطمه، رضایی فاطره. مطالعۀ فیتوشیمیایی و اثر ضدتکثیری عصارۀ هیدروالکلی گیاه پرسیاوشان روی رده های سلولی MCF-7 و MRC-5. یافته های نوین در علوم زیستی. 1397; 5 (2) :118-127

URL: http://nbr.khu.ac.ir/article-1-3059-fa.html


دانشگاه آزاد اسلامی واحد بابل
چکیده:   (1364 مشاهده)
با افزایش بیماری سرطان و عوارض جانبی درمان­ های رایج محققان به دنبال یافتن روش ­های درمانی با کمترین عوارض هستند به همین علت گیاهان دارویی اهمیت بالایی پیدا کرده اند. گیاه پرسیاوشان (Adiantum capillus-veneris L.) دارای ترکیبات تری ترپنوئیدی است که خاصیت ضدتومور دارند. هدف این مطالعه بررسی اثر کشندگی عصارۀ گیاه بر ردۀ سلولی سرطان پستان (MCF-7) و سلول های نرمال فیبروبلاست (MRC-5) با استفاده از روش MTT است. گیاه پرسیاوشان از روستای مرزون آباد جمع­ آوری و در دمای C° ٤٠ خشک شد. با استفاده از دستگاه سوکسله و اتانول ٩٦ درصد از بخش هوایی و زیرزمینی گیاه  پرسیاوشان عصارۀ هیدروالکلی تهیه و از این عصاره سه فراکسیون هگزان، کلروفرم واتیل استات آماده شد. همچنین، با استفاده از عصارۀ هیدروالکلی آن، ترکیبات گیاه توسط دستگاه GC-Mass شناسایی شد. نتایج حضور ترکیبات پلی­فنلی، ترپنویید، اسیدهای چرب، موم، الکالویید، ترکیبات N- اکسید و فیبررا نشان می­دهد که این گیاه دارای خاصیت آنتی­ اکسیدانی قوی است. نتایج آزمایش MTT نشان داد که از یک طرف این عصاره اثر کشندگی وابسته به دوز بر سلول ­های MCF-7 دارد و قادر به از بین بردن سلول­ های سرطانی بوده و از طرف دیگر اثر آن بر سلول­ های سرطانی بیشتر از ردۀ نرمال است. همچنین میزان IC50 عصاره ­های بخش هوایی و زیرزمینی روی هر دو رده تفاوت معناداری را نشان می­دهند. خواص کشندگی آن قابل مقایسه با داروی ضدسرطان سیس پلاتین است.    
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نوع مطالعه: مقاله پژوهشی | موضوع مقاله: میکروبیولوژی
دریافت: 1396/10/18 | ویرایش نهایی: 1397/7/10 | پذیرش: 1396/10/18 | انتشار: 1396/10/18 | انتشار الکترونیک: 1396/10/18

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