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Hassanpour H, Shokrzadeh Lamuki M, Tabari R, Rezaee F, Rezaee F. The phytochemical study and antiprolifrative effect of hydroalcoholic extract of Adiantum capillus-veneris L. on MCF-7 and MRC-5 cell lines. nbr 2018; 5 (2) :118-127
URL: http://nbr.khu.ac.ir/article-1-3059-fa.html
URL: http://nbr.khu.ac.ir/article-1-3059-fa.html
حسن پور حسانه، شکرزاده لموکی محمد، طبری رضا، رضایی فاطمه، رضایی فاطره. مطالعۀ فیتوشیمیایی و اثر ضدتکثیری عصارۀ هیدروالکلی گیاه پرسیاوشان روی رده های سلولی MCF-7 و MRC-5. یافته های نوین در علوم زیستی. 1397; 5 (2) :118-127
دانشگاه آزاد اسلامی واحد بابل
چکیده: (6587 مشاهده)
با افزایش بیماری سرطان و عوارض جانبی درمان های رایج محققان به دنبال یافتن روش های درمانی با کمترین عوارض هستند به همین علت گیاهان دارویی اهمیت بالایی پیدا کرده اند. گیاه پرسیاوشان (Adiantum capillus-veneris L.) دارای ترکیبات تری ترپنوئیدی است که خاصیت ضدتومور دارند. هدف این مطالعه بررسی اثر کشندگی عصارۀ گیاه بر ردۀ سلولی سرطان پستان (MCF-7) و سلول های نرمال فیبروبلاست (MRC-5) با استفاده از روش MTT است. گیاه پرسیاوشان از روستای مرزون آباد جمع آوری و در دمای C° ٤٠ خشک شد. با استفاده از دستگاه سوکسله و اتانول ٩٦ درصد از بخش هوایی و زیرزمینی گیاه پرسیاوشان عصارۀ هیدروالکلی تهیه و از این عصاره سه فراکسیون هگزان، کلروفرم واتیل استات آماده شد. همچنین، با استفاده از عصارۀ هیدروالکلی آن، ترکیبات گیاه توسط دستگاه GC-Mass شناسایی شد. نتایج حضور ترکیبات پلیفنلی، ترپنویید، اسیدهای چرب، موم، الکالویید، ترکیبات N- اکسید و فیبررا نشان میدهد که این گیاه دارای خاصیت آنتی اکسیدانی قوی است. نتایج آزمایش MTT نشان داد که از یک طرف این عصاره اثر کشندگی وابسته به دوز بر سلول های MCF-7 دارد و قادر به از بین بردن سلول های سرطانی بوده و از طرف دیگر اثر آن بر سلول های سرطانی بیشتر از ردۀ نرمال است. همچنین میزان IC50 عصاره های بخش هوایی و زیرزمینی روی هر دو رده تفاوت معناداری را نشان میدهند. خواص کشندگی آن قابل مقایسه با داروی ضدسرطان سیس پلاتین است.
نوع مطالعه: مقاله پژوهشی |
موضوع مقاله:
میکروبیولوژی
دریافت: 1396/10/18 | ویرایش نهایی: 1397/7/10 | پذیرش: 1396/10/18 | انتشار: 1396/10/18 | انتشار الکترونیک: 1396/10/18
دریافت: 1396/10/18 | ویرایش نهایی: 1397/7/10 | پذیرش: 1396/10/18 | انتشار: 1396/10/18 | انتشار الکترونیک: 1396/10/18
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