The phytochemical study and antiprolifrative effect of hydroalcoholic extract of Adiantum capillus-veneris L. on MCF-7 and MRC-5 cell lines

Hassanpour, Hesane; Shokrzadeh Lamuki, Mohammad; Tabari, Reza; Rezaee, Fatemeh; Rezaee, Fatereh

doi:10.29252/nbr.5.2.118

The phytochemical study and antiprolifrative effect of hydroalcoholic extract of Adiantum capillus-veneris L. on MCF-7 and MRC-5 cell lines

Authors

Hesane Hassanpour ¹

Mohammad Shokrzadeh Lamuki ²

Reza Tabari ¹

Fatemeh Rezaee ¹

Fatereh Rezaee ¹

¹ Babol branch of Azad University

² Mazandaran Medical Science University

https://doi.org/10.29252/nbr.5.2.118

Abstract

With the increase of cancer disease and the side-effects of the current treatments, researchers are attempting to find methods with less side-effect. The fern Adiantum capillus-veneris L. has triterpenoid compounds which have anti-tumor characteristics. This study aims to investigate the fatal effect of the extract of this plant on breast cancer (MCF-7) and normal (MRC-5) cell lines using MTT method. A. capillus-veneris specimens were collected from Marzoon Abad Village and were dried at the temperature of 40° C by the use of Soxhlet and Ethanol % 96. Hydroalcoholic extract was produced from the aerial and underground parts of this plant and 3 fractions, i.e., Hexane, Chloroform and Ethyl acetate, were prepared from the extract. The compounds of the plant extract were identified by GC-Mass. The results demonstrate the presence of polyphenolic compounds, terpenoid, fatty acids, wax, alkaloid, N-oxide and fibers, with strong antioxidant effect. The results of the MTT test proved that this extract had a dose-depending fatal effect on the MCF-7 cells and is capable of eradicating the cancer cells. On the other hand, its effect on cancer cells is more than its effect on normal cells. Also, IC₅₀ in both of cell lines induced by the extracts of aerial and underground parts showed a significant difference. The fatal characteristics of the extract are comparable with the cisplatin anti-cancer drug.

Keywords

antioxidant

breast cancer

cisplatin

GC-Mass

Polypodiaceae

20.1001.1.24236330.1397.5.2.9.9

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