دوره 5، شماره 2 - ( 6-1397 )                   جلد 5 شماره 2 صفحات 127-118 | برگشت به فهرست نسخه ها


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Hassanpour H, Shokrzadeh Lamuki M, Tabari R, Rezaee F, Rezaee F. The phytochemical study and antiprolifrative effect of hydroalcoholic extract of Adiantum capillus-veneris L. on MCF-7 and MRC-5 cell lines. NBR 2018; 5 (2) :118-127
URL: http://nbr.khu.ac.ir/article-1-3059-fa.html
حسن پور حسانه، شکرزاده لموکی محمد، طبری رضا، رضایی فاطمه، رضایی فاطره. مطالعۀ فیتوشیمیایی و اثر ضدتکثیری عصارۀ هیدروالکلی گیاه پرسیاوشان روی رده های سلولی MCF-7 و MRC-5. یافته‌ های نوین در علوم زیستی. 1397; 5 (2) :118-127

URL: http://nbr.khu.ac.ir/article-1-3059-fa.html


دانشگاه آزاد اسلامی واحد بابل
چکیده:   (4691 مشاهده)
با افزایش بیماری سرطان و عوارض جانبی درمان­ های رایج محققان به دنبال یافتن روش ­های درمانی با کمترین عوارض هستند به همین علت گیاهان دارویی اهمیت بالایی پیدا کرده اند. گیاه پرسیاوشان (Adiantum capillus-veneris L.) دارای ترکیبات تری ترپنوئیدی است که خاصیت ضدتومور دارند. هدف این مطالعه بررسی اثر کشندگی عصارۀ گیاه بر ردۀ سلولی سرطان پستان (MCF-7) و سلول های نرمال فیبروبلاست (MRC-5) با استفاده از روش MTT است. گیاه پرسیاوشان از روستای مرزون آباد جمع­ آوری و در دمای C° ٤٠ خشک شد. با استفاده از دستگاه سوکسله و اتانول ٩٦ درصد از بخش هوایی و زیرزمینی گیاه  پرسیاوشان عصارۀ هیدروالکلی تهیه و از این عصاره سه فراکسیون هگزان، کلروفرم واتیل استات آماده شد. همچنین، با استفاده از عصارۀ هیدروالکلی آن، ترکیبات گیاه توسط دستگاه GC-Mass شناسایی شد. نتایج حضور ترکیبات پلی­فنلی، ترپنویید، اسیدهای چرب، موم، الکالویید، ترکیبات N- اکسید و فیبررا نشان می­دهد که این گیاه دارای خاصیت آنتی­ اکسیدانی قوی است. نتایج آزمایش MTT نشان داد که از یک طرف این عصاره اثر کشندگی وابسته به دوز بر سلول ­های MCF-7 دارد و قادر به از بین بردن سلول­ های سرطانی بوده و از طرف دیگر اثر آن بر سلول­ های سرطانی بیشتر از ردۀ نرمال است. همچنین میزان IC50 عصاره ­های بخش هوایی و زیرزمینی روی هر دو رده تفاوت معناداری را نشان می­دهند. خواص کشندگی آن قابل مقایسه با داروی ضدسرطان سیس پلاتین است.    
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نوع مطالعه: مقاله پژوهشی | موضوع مقاله: میکروبیولوژی
دریافت: 1396/10/18 | ویرایش نهایی: 1397/7/10 | پذیرش: 1396/10/18 | انتشار: 1396/10/18 | انتشار الکترونیک: 1396/10/18

فهرست منابع
1. Abdullaev, F.I. 2001. Plant-derived agents cancer. In: Gupta SK, ed. Pharmacology and therapeutics in the New Millennium. – New Delhi: Narosa Publishing House. pp 345-354.
2. Abdullaev, F.I., Riveron-Negretts, L., Rotenburd-Belacortu, V. and Kasumov, F.J. 2000. Saffron as chemo preventive agent. In: Abdullaev FI. Riveron-Negretts L. Rotenburd-Belacortu V, Kasumov FJ, eds. – Food of 21st century: Food and resource technology environment. China: Ligh Industry Press. 185-195.
3. Ahmad, E., Hussin, M., Ahmad, M.S.A., Ashraf, M.Y., Ahmad, R., and Ali, A. 2008. Spatio temporal variations in physiochemical attributes of Adiantum capillus subsp. veneris from Soone valley of salt range (Pakistan). – Pak. J. Bot. 40: 1387-1398.
4. Ana-Maria, F. and Dietrich, B. 2011. Cisplatin as an anti-tumor drug: cellular mechanisms of activity, drug resistance and induced side effects. – J. Cancers 3: 1351-1371.
5. Ansari, R. and Ekhlasi-Kazaj, K. 2012. Adiantum capillus-veneris. L: phytochemical cons-tituents, tr-aditional uses and pharmacological properties. – J. Adv. Sci. Res. 3: 15-20.
6. Bear, W.L. and Teel, R.W. 2000. Effects of citrus flav-onoids on the mutagenicity of heterocyclic amines and on cytochrome P450 1A2 activity. – Anticancer Res. 20: 3609-3614.
7. Chiba, K., Kawakami, K. and Tohyama, K. 1998. Simultaneous evaluation of viability by neutral red, MTT and crystal violet staining assays of the same cells. – Toxicol. in Vitro 12: 251-258.
8. Craig, W.J. 1999. Health promoting properties of common herbs. – American. J. Clin. Nutr. 70: 491-499.
9. Dominguez, M., Keck, A.S., Jeffery, E.H. and Cespedes, C.L. 2010. Effects of extracts, flavonoids and iridoids from Penstemon gentianoides (Plantaginaceae) on inhibition of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) in LPS- Activated RAW 264.7 macrophage cells and their antioxidant activity. – Bol. Latin. Car. Plant Med. Aromát. 9: 397-413.
10. Durmaz, R., Deliorman, S., Isiksoy, S., Uyar, R., Erol, K. and Tel, E. 1999. Antiproliferative properties of the Lazaroids U-83836E and U-74389G on glioma cells in vitro. – Pathol. Oncology Res. 5: 223-228.
11. Eisenberg, D., Kessler, R.C., Foster, C., Norlock, F.E. and Calkins, D.R. 1993. Unconventional medicine in United States: prevalence, cost and patterns of use. – N Engl. J. Med. 328: 246-252.
12. EL-Alfy and Mahran, G.H. 1998. Chemical composition and antimicrobial activity of the volatile oil and extracts of fronds of Adiantum capillus-veneris. – Bulletin of Faculty 3: 555-572.
13. Freshney, R.I. 2000. Culture of animal cells: manual of basic technique. 4th ed. – Wiley- Liss. 624.
14. Gharavi, M.R.A. and Moatar, F. 1993. The effect of maidenhair extract on frog. – Myocardium. J. Isfahan Med. Faculty 37: 17-23.
15. Gharavi, M.R.A. and Moatar, F. 1994. The acute and chronic effect of Adiantum capilus subsp. veneris L. Extract on hemoglobin, hematocrite, means corpuscle volume, prothrombin time and partial thromboplastin time in rat. – J. Zanjan Uni. Med. Sci. 2: 6 -13.
16. Guha, P., Mukhopadhyay, R., Pal, P.K. and Gupta, K. 2004. Antimicrobial activity of crude extracts and extracted phenols from gametophyte and sporophytic plant parts of Adiantum capillus subsp. veneris. – Allelopathy J. 13: 57-66.
17. Haider, S., Nazreen, S., Alam, M.M., Gupta, A., Hamid, H. and Alam, M.S. 2011. Anti-inflammatory and anti-nociceptive activities of hydroalcoholic extract and its various fractions from Adiantum capillus subsp. veneris. – Linn. J. Ethnopharmacology 138: 741-747.
18. Hickey, M., Peate, M., Saunders, C.M. and Friedlander, M. 2009. Breast cancer in young women and its impact on reproductive function. – Hum. Reprod. Update. 15: 323-339.
19. Hussain, M.M., Muthuprasanna, P., Srinivasarao, T., Velraj, M., Shanmugapandian, P. and Suriaprabha, K. 2008. Analgesic and anti-inflammatory activity of Adiantum venustum. – Res. Rev. Biosci. J. 2: 102-104.
20. Hussain, M.M., Ahmad, B., Rashid, E., Hashim, S., Marwat, K.H.B. and Jan, A. 2014. In Vitro Anti-bacterial activitiy of methanol and Tagetes patula against multidrug resistant bacterial strains. – Pak. J. Bot. 46: 363-368.
21. Jain, S.R. and Sharma, S.N. 1967. Hypoglycaemic drugs of Indian indigenous origin. – Planta Med. 15: 439 - 442.
22. Katalinic, V., Milos, M., Kulisic, T. and Jukic, M. 2006. Screening of 70 medicinal plant extracts for antio-xidant capacity and total phenols. – Food Chem. 94: 550-557.
23. Kumar, M., Jain, S., Singh, T., Pande, M. and Nema, N. 2014. Neuropharmacological screening of fronds of Adiantum capillus subsp. Veneris L. – Scholars Research Library 6:167-175.
24. Ljubuncic, P., Dakwar, S., Portnaya, I., Cogan, U., Azaizeh, H. and Bomzon, A. 2006. Aqueous extracts of Teucriumpolium possess remarkable antioxidant activity in vitro. – Evid. Bas. Complement Alternat. Med. 3: 329-338.
25. Lowe, S.W. and Lin, A.W. 2000. Apoptosis in cancer. – Carcinogenesis 21: 485-95.
26. Mabeza, G.F. and Macfarlane. J. 2003. Pulmonary actinomycosis. – Europ. Resp. J. 21: 545–555.
27. Mahmoud, M.J., Jawad, A.L.M., Hussain, A.M., Al-Omari, M. and Al-Naib, A. 1989. In vitro antimicrobial activity of salsola rosmarinus and Adiantum capillus subsp. veneris. – Pharmaceut. Biol. 27: 14-16.
28. Mahran, G.H. 1999. Chemical composition and antimicrobial activity of Adiantum capillus subsp. veneris. – Bultan of Agriculture University of Cairo 43: 451-457.
29. Momeni, T. 2001. Phitology of extracts. 1st ed. Tehran: Shahid Farhad Reza Press. pp 218-220.
30. Nakane, T., Maeda, Y., Ebihara, H., Arai, Y., Masuda, K., Takano, A., Ageta, H., Shiojima, K., Cai, S.Q. and Abdel Halim, O.B. 2002. Fern constituents: Triterpenoids from Adiantum capillus subsp. veneris. – Chem. Pharmaceut. Bul. 50: 1273–1275
31. Noubarani, M., Rostamkhani, H., Erfan, M., Kama-linejad, M., Babaeian, M. and Salamzadeh, J. 2014. Effect Adiantum capillus subsp. veneris Linn on an animal model of testosterone-induced hair loss. – Iranian. J. Pharm. Res. 13: 113-118.
32. Nyarko, H.D., Barku, V.Y.A. and Batama, J. 2012. Antimicrobial examinations of Cymbopogon citratus and Adiantum capilus subsp. veneris used in Ghan-aian folkloric medicine. – Life Sci. 2: 115-121.
33. Ocvirk, S., Kistler, M., Khan, S., Hayder Talukder, S. and Hauner, H. 2013. Traditional medicinal plants used for the treatment of diabetes in rural and urban areas of Dhaka, Bangladesh an ethnobotanical survey. – J. Ethnobiol. Ethnomed. 9: 1-8.
34. Pourmorad, F., Hosseinimehr, S.J. and Shahabimajd, N. 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. – Afr. J. Biotech. 5: 1142-1145.
35. Rajurkar, N.S. and Gaikwad, K. 2012. Evaluation of phytochemical, antioxidant activity and elemental content of Adiantum capillus subsp. veneris leaves. – J. Chem. Pharm. Res. 4:365-374.
36. Ranjan, V., Vats, M., Gupta, N. and Sardana, S. 2014. Antidiabetic potential of whole plant of Adiantum capillus subsp. veneris L. In streptozotocin induced diabetic rats. – Inter. J. Pharm. Clin. Res. 6: 341-347.
37. Reddy, A., Pereira, A. and Gil, C. 2001. Antibacterial activity of essential oil and extracts of Adiantum lunulatumon gram positive bacteria. – Biochem. 4: 221-227.
38. Resende, F.A., Vilegas, W., Dos Santos, L.C. and Varanda, E.A. 2012. Mutagenicity of flavonoids assayed by bacterial reverse mutation (Ames) test. – Molecules 17: 5255-5268.
39. Rezaei, F., shokrzadeh, M., Majd, A. and Nezhadsattari, T. 2014. Cytotoxic activity of ripe and unripe cornellian cherry (Cornus mas L.) Fruits. – Ergenal. Article Emergencias 3: 98-104.
40. Rezaei, F., Shokrzadeh, M., Majd, A. and Nezhadsattari, T. 2014. Cytotoxic effect of hydroalcoholic extract of cornus mas L. fruit on MCF7, HepG2 and CHO cell line by MTT assay. – J. Mazandaran Univ. Med. Sci. 24: 130-138.
41. Seidel, V. 2006. Initial and bulk extraction. In: Sarker SD, Latif Z, Gray AI. Natural product isolation. 2nd ed. New Jersey: Humana Press. 27-46.
42. Seyed Noori, T., Zahmatkesh, T. and Molaei, T. 2008. Assessment breast cancer risk using gill modeles. – J. Iran Breast Disease 2: 53-57.
43. Shokrzadeh, M., Azadbakht, M., Ahangar, N. and Naderi, H. 2009. Extract of Juniperus sabina compared with hydroalcoholic extract of Taxus baccata and Cisplatin on normal and cancer cell lines. – Planta Med. 75: 986-987.
44. Shokrzadeh, M., Parvaresh, A., Shahani, S., Habibi, O. and Zalzar, Z. 2013. Cytotoxic effects of Lagenaria siceraria Standl extract on cancer cell line. – J. Mazandaran Univ. Med. Sci. 23: 225-230.
45. Singh, M., Singh, N., Khare, P.B. and Rawat, A.K.S. 2008. Antimicrobial activity of some important Adiantum species used traditionally in indigenous systems of medicine. – J. Ethnopharmacol. 115: 327-329.
46. Sukhramani, P.S., Sukhramani, P.S., Tirthani, S.R., Desai, S.A. and Suthar, M.P. 2011. Biological cytotoxicity evaluation of spiro [azetidine-2, 3’-indole]-2’, 4(1’H)-dione derivatives for anti-lung and anti-breast cancer activity. – Der Pharmacia Letter 3: 236-243.
47. Sundaram, S.G. and Milner, J.A. 1993. Impact of organosulfur compounds in garlic on canine mammary tumor cells in culture. – Cancer Lett. 74: 85-90.
48. Tang, J., Wang, C.K., Pan, X., Yan, H., Zeng, G. and Xu, W. 2010. Isolation and characterization of cytotoxic cyclotides from viola tricolor. – Peptides. 31: 1434-1440.
49. Tantawy, E.L. 2003. Antimicrobial effects of alcoholic extracts of Adiantum capillus subsp. veneris. – Biochem. J. 2: 256-261
50. Tian, S., Shi, Y., Zhou, X., Ge, L. and Upur, H. 2011. Total polyphenolic (flavonoids) content and antio-xidant capacity of different Ziziphora clino-podioides lam extracts. – Pharmacogn. Mag. 7: 65-68.
51. Vijayalakshmi, A. and Kiran Kumar, Y. 2013. Evaluation of goitrogenic and antithyroidal effect of the fern Adiantum capillus subsp. veneris. Revista Brasileira de Farmacognosia Brazilian. – J. Pharm-acognosy. 23: 802-810.
52. Viral, D., Shivanand, P. and Jivani, N.P. 2011. Anticancer evaluation of Adiantum venustum Don. – J. Young Pharm. 3: 48-54.
53. Yuan, Q., Zhang, X., Liu, Z., Song, S., Xue, P., Wang, J. and Ruan, J. 2013. Ethanol extract of Adiantum capillus subsp. veneris L. suppresses the production of inflammatory mediators by inhibiting nf-κb activation. – J. Ethnopharmacol. 147: 603-611.
54. Zhang, P., Summer, W.R., Bagby, G.J. and Nelson, S. 2000. Innate immunity and pulmonary host defense. – Immunology Rev. 173: 39-51.

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