Reducing the toxic effects of Chromium in the growing medium of costmary (Tanacetum balsamita) by means of chromium purifying bacteria

Valizadeh Kamran, Rana; Vojodi Mehrabani, Lamia; Abdoulzadeh Fard, Ali; Tarinejad, Alireza

Reducing the toxic effects of Chromium in the growing medium of costmary (Tanacetum balsamita) by means of chromium purifying bacteria

Authors

Rana Valizadeh kamran ¹

Lamia Vojodi Mehrabani ²

Ali Abdoulzadeh Fard ¹

Alireza Tarinejad ¹

¹ Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran

² Department of Agronomy and Plant Breeding, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran

Abstract

Rana Valizadeh Kamran¹, Lamia Vojodi Mehrabani², Ali Aryan¹ & Alireza Tarinejad¹

¹

Corresponding author: Rana Valizadeh Kamran, rana.valizadeh@gmail.com

Abstract. Bioremediation is a promising strategy to reduce the concentration of heavy metals that their increase in the soil was the result of the development of industries and factories in the area, threatening the environment and human health. To investigate the effect of the heavy metal chromium and the reduction of its toxic effects by bacteria (at two levels of the absence of bacteria and the presence of bacteria in Hoagland's solution), a factorial experiment was conducted as a completely randomized design with three replications and the morphological, physiological traits and plant elements were measured in the applied treatments. The results showed that the experimental treatments did not affect plant yield traits, fresh weight, stem length, and leaf length. Leaf width, chlorophyll a, b, and plant phosphorus content decreased under chromium stress and increased with bacterial treatment. Hydrogen peroxide, malondialdehyde, ascorbate peroxidase, superoxide dismutase, catalase, proline, solid soluble substances, phenol, flavonoid, and anthocyanin, as well as the content of plant elements such as chromium, nitrogen, and potassium, increased due to the chromium treatment. Using bacteria in the culture medium containing chromium, significantly decreased the hydrogen peroxide and malondialdehyde, indicating a reduction in the oxidative stress. The non-enzymatic and enzymatic antioxidants of plats in the bacterial treatments increased, indicating bacteria's role in strengthening the plant's antioxidant system. The chromium content of the plant decreased after the use of bacteria. The results showed the positive effect of using chromium-purifying bacteria in the environment of plant cultivation in reducing the harmful effects of chromium heavy metal stress.

Keywords

bioremediation

elemental content

enzyme

malondialdehyde

total soluble solid

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