یافته های نوین در علوم زیستی

کاهش اثرات سمی کروم در محیط رشد شاهسپرم توسط باکتری تصفیه­‌کننده کروم

نویسندگان

رعنا ولی زاده کامران 1
لمیا وجودی مهربانی 2
علی عبدالزاده فرد 1
علیرضا تاری نژاد 1

1 گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

2 گروه زراعت و اصلاح نباتات دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

چکیده
زیست پالایی یک استراتژی امیدوار کننده برای کاهش غلظت فلزات سنگینی است که با توسعه صنایع و کارخانه­ها در خاک افزایش می­یابند و تهدیدی برای محیط زیست و سلامتی انسان هستند. برای بررسی تاثیر فلز سنگین کروم وکاهش اثرات سمی آن توسط باکتری (در دو سطح بدون وجود باکتری و وجود باکتری در محلول هوگلند آزمایش فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار انجام شد و صفات ریخت شناسی، فیزیولوژیکی و عناصر گیاه درتیمارهای اعمال شده اندازه­گیری شد. نتاج نشان داد صفات عملکرد گیاه، وزن تر، طول ساقه و طول برگ تحت تاثیر تیمارهای آزمایشی قرار نگرفتند. عرض برگ، کلروفیل a، کلروفیل b و محتوی فسفر گیاه که تحت تنش کروم کاهش یافته بودند با تیمار باکتری، افزایش یافتند. هیدروژن پراکساید، مالون دی آلدیید، اسکوربات پراکسیداز، سوپراکسید دیسموتاز، کاتالاز، پرولین، مواد محلول جامد، فنل، فلاونویید و آنتو سیانین و همچنین محتوای عناصر گیاه از قبیل کروم، ازت و پتاسیم در اثر تیمار کروم افزایش یافتند. با استفاده از باکتری در محیط کشت حاوی کروم، هیدروژن پراکساید و مالون دی آلدیید به طور معنی داری کاهش یافت که نشان از کاهش تنش اکسیداتیو بود. آنتی اکسیدان های غیرآنزیمی و آنزیمی تیمارهای باکتری­دار افزایش پیدا کردند که نشان از نقش باکتری در تقویت سیستم آنتی­اکسیدانی گیاه داشت. محتوی کروم گیاه بعد از استفاده از باکتری کاهش پیدا کرد. نتایج حاصل نقش مثبت کاربرد باکتری تصفیه کننده کروم درمحیط کشت گیاه در کاهش اثرات منفی تنش فلز سنگین کروم را نشان داد.



کلیدواژه‌ها

آنزیم
زیست پالایی
عناصر
مالون دی­آلدئید
مواد جامد محلول

عنوان مقاله English

Reducing the toxic effects of Chromium in the growing medium of costmary (Tanacetum balsamita) by means of chromium purifying bacteria

نویسندگان English

Rana Valizadeh kamran 1
Lamia Vojodi Mehrabani 2
Ali Abdoulzadeh Fard 1
Alireza Tarinejad 1
1 Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
چکیده English





Rana Valizadeh Kamran1, Lamia Vojodi Mehrabani2, Ali Aryan1 & Alireza Tarinejad1

1

Corresponding author: Rana Valizadeh Kamran, rana.valizadeh@gmail.com



Abstract. Bioremediation is a promising strategy to reduce the concentration of heavy metals that their increase in the soil was the result of the development of industries and factories in the area, threatening the environment and human health. To investigate the effect of the heavy metal chromium and the reduction of its toxic effects by bacteria (at two levels of the absence of bacteria and the presence of bacteria in Hoagland's solution), a factorial experiment was conducted as a completely randomized design with three replications and the morphological, physiological traits and plant elements were measured in the applied treatments. The results showed that the experimental treatments did not affect plant yield traits, fresh weight, stem length, and leaf length. Leaf width, chlorophyll a, b, and plant phosphorus content decreased under chromium stress and increased with bacterial treatment. Hydrogen peroxide, malondialdehyde, ascorbate peroxidase, superoxide dismutase, catalase, proline, solid soluble substances, phenol, flavonoid, and anthocyanin, as well as the content of plant elements such as chromium, nitrogen, and potassium, increased due to the chromium treatment. Using bacteria in the culture medium containing chromium, significantly decreased the hydrogen peroxide and malondialdehyde, indicating a reduction in the oxidative stress. The non-enzymatic and enzymatic antioxidants of plats in the bacterial treatments increased, indicating bacteria's role in strengthening the plant's antioxidant system. The chromium content of the plant decreased after the use of bacteria. The results showed the positive effect of using chromium-purifying bacteria in the environment of plant cultivation in reducing the harmful effects of chromium heavy metal stress.




کلیدواژه‌ها English

bioremediation
elemental content
enzyme
malondialdehyde
total soluble solid
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

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