Molecular phylogeny and taxonomy of species of Cousinia section Stenocephalae (Asteraceae) in NE of Iran

Kalouti, Simin; Kazempour-Osaloo, Shahrokh; Attar, Farideh; Susanna, Alfonso; Garcia-Jacas, Núria

doi:10.52547/nbr.8.1.56

Molecular phylogeny and taxonomy of species of Cousinia section Stenocephalae (Asteraceae) in NE of Iran

Authors

Simin Kalouti ¹

Shahrokh Kazempour-Osaloo ¹

Farideh Attar ²

Alfonso Susanna ³

Núria Garcia-Jacas ³

¹ Tarbiat Modares University

² University of Tehran

³ Botanic Institute of Barcelona (CSIC-ICUB)

https://doi.org/10.52547/nbr.8.1.56

Abstract

In this study, the phylogeny and morphological variation of 6 species of the genus Cousinia sect. Stenocephalae, i.e., C. hypopolia, C. decipiens, C. esfandiarii, C. renominata, C. stahliana and C. daryoushiana, distributed in NE regions of Iran, were investigated. ITS, ETS and rpl32-trnL ^(UAG) sequences for 12 species (17 specimens) were generated. Phylogenetic interspecies relationships were constructed by Bayesian method and likelihood analysis. The specimens were also investigated in terms of morphological characters. Despite very similar morphological characters and geographical distributions, the separation of these species was confirmed by the obtained results of the molecular data (combined data of plastid and nuclear sequences). On the basis of the present study, the above-mentioned species were almost separated from each other by morphological characters and molecular data. A diagnostic key and the description of these species were also presented. These results did not conform with the previous palynological and anatomical studies of the species.

Keywords

Bayesian analysis

chloroplast sequences

geographical distribution

morphological characters

nuclear sequences

20.1001.1.24236330.1400.8.1.9.8

Attar, F., Ghahreman, A., Saber, A. & Zarre, S. 2005. A new species of the genus Cousinia (Compositae, sect. Stenocephalae) from SW of Iran. Iranian Journal of Botany 11: 65-69.
Attar, F. 2011a. Taxonomic revision of Cousinia sect. Albidae Bunge (Asteraceae) in the flora of Iran. Iranian Journal of Botany 17: 150-157.
Attar, F. 2011b. New taxa of Cousinia Cass., Sect. Stenocephalae Bunge (Asteraceae) from Iran. Taxonomy and Biosystematics 3: 59-68.
Baldwin, B.G & Marcos, S. 1998. Phylogenetic utility of the external transcribed spacer (ETS) of 18S–26S rDNA: Congruence of ETS and ITS trees of Calycadenia (Compositae). Molecular Phylogenetics and Evolution 10: 449-463.
Borchsenius, F. 2009. FastGap 1.2. Department of biosciences, Aarhus University, Denmark. Published online at http://www.aubot.dk/FastGap_home.htm
Bordbar, F., Baqeri, M. & Mirtadzadini, M. 2017. New chromosome counts in Cousinia (Asteraceae) from Flora of Iran. Cytologia 82: 485-488.
Djamali, M., Baumel, A., Brewer, S., Jackson, S.T, Kadereit, J.W., López-Vinyallonga, S., Mehregan, I., Shabanian, E. & Simakova, A. 2012. Ecological implications of Cousinia Cass. (Asteraceae) persistence through the last two glacial–interglacial cycles in the continental Middle East for the Irano-Turanian flora. Review of Palaeobotany and Palynology 172: 10-20.
Djavadi, S.B. & Attar, F. 2006. Two new species of Cousinia Cass. of sect. Stenocephalae Bunge from Iran. Feddes Repertorium 117: 453-458.
Djavadi, S.B. 2007. New or rare chromosome count in ten species of Cousinia from Iran. Rostaniha 8: 19-27.
Djavadi, S.B. & Attar, F. 2010. New chromosome counts in the genus Cousinia (Asteraceae, Cardueae) from Iran. Willdenowia 40: 351-357.
Djavadi, S.B. 2012. New chromosome count in Cousinia (Asteraceae, Cardueae) section Cynaroideae and Stenocephalae from Iran. Iranian Journal of Botany 18: 86-93.
Doyle, J.J. & Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Buletin 19: 11-15.
Edgar, R.C. 2004. Muscle: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792-1797.
Faris, J.S. Kallersjo, M. Kluge, A.G. & Bult, C. (1995). Testing significance of incongruence. Cladistics 10: 315-319.
Ghaffari, S.M. & Sanaei Chariat-Panahi, M. 1985. Chromosome count of some angiosperms from Iran. Iranian Journal of Botany 3: 67-73.
Ghaffari, S.M. & Djavadi, S.B. 1998. Chromosome studies and distribution of nine species of Cousinia section Stenocephlae (Asteraceae) in Iran. Bulletin de la Société Neuchâteloise des Sciences Naturelles 121: 61-68.
Ghaffari, S.M, Susanna, A. and Garcia-Jacas, N. 2006. New chromosome counts in the genus Cousinia (Asteraceae) from Iran. Botanical Journal of the Linnean Society 151: 411-419.
Knapp, H.D. 1987. On the distribution of the genus Cousinia (Compositae). Plant Systematic and Evolution 155: 15-25.
Lee, J., Baldwin, B.G. & Gottlieb, L. 2002. Phylogeny of Stephanomeria and related genera (Compositae–Lactuceae) based on analysis of 18S–26S nuclear rDNA ITS and ETS sequences. American Journal of Botany 89: 160-168.
Linder C.R., Goertzen, L.R., Heuvel, B.V., Francisco-Ortega, J. & Jansen, R.K. 2000. The complete external transcribed spacer of 18S–26S rDNA: amplification and phylogenetic utility at low taxonomic levels in Asteraceae and closely allied families. Molecular Phylogenetics and Evolution 14: 285-303.
López-Vinyallonga, S., Mehregan, I., Garcia-Jacas, N., Tscherneva, O., Susanna, A. & Kadereit, J.W. 2009. Phylogeny and evolution of the Arctium-Cousinia complex (Compositae, Cardueae-Carduinae). Taxon 58: 153-171.
López-Viyallonga, S. Romaschenko, K., Susanna, A. & Garcia-Jacas, N. 2011. Systematics of the Arctioid group: Disentangling Arctium and Cousinia (Cardueae, Carduinae). Taxon 60: 539-554.
Marcos, S. & Baldwin, B.G. 2001. Higher-Level Relationships and Major Lineages of Lessingia (Compositae, Astereae) Based on Nuclear rDNA Internal and External Transcribed Spacer (ITS and ETS) Sequences. Systematic Botany 26: 168-183.
Miller, M.A., Pfeiffer, W., & Schwartz, T. 2010. Creating the CIPRES science gateway for inference of large phylogenetic trees. In Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, LA, 8 pp, available at: htpp://www.phylo. org.
Nylander, J.A.A. 2004. MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre. Uppsala University, Uppsala.
Parsa, A. 1945. Cousinia in flore de L' Iran. vol. 3. Tehran University Press, pp. 480.
Post, G.E. 1933. Flora of Syria, Palestine and Sinai, vol. II. American University of Beirut, pp. 84-87.
Rechinger, K.H. 1986. Cousinia morphology, taxonomy and phytogeographic implications. Proceedings of the Royal Society. Edinburgh, B., Biological Sciences 89: 45-58.
Rechinger, K.H. 1972. Compositae-Cynareae І: Cousinia. -In Rechinger K.H. (ed.), Flora Iranica vol. 90. Akad. Druck- und Verlagsanstalt, Graz, 329 pp.
Rechinger, K.H. 1979. Compositae-Cynareae ІІІ: Cousinia. –In: Rechinger K.H. (ed.), Flora Iranica vol. 139a. Akad. Druck- und Verlagsanstalt, Graz, pp: 105-156.
Saber, A. 2005. Biosystematical studies on the Cousinia section Stenocephalae (Asteraceae) in Iran. M.Sc. thesis, University of Tehran.
Saber, A., Attar, F. & Djavadi, S.B. 2009. Studies of pollen grains in the sect. Stenocephalae (Cousinia Cass. Asteraceae) in Iran. Iranian Journal of Botany 15: 114-128.
Sang, T., Crawford, D.J. & Stuessy, T.F. 1995. Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution. Proceedings of the National Academy of Sciences of the United States of America 92: 6813-6817.
Shaw J., Lickey, E., Schilling, E. & Small, R.L. 2007. Comparison of whole chloroplast genome sequence to choose noncoding regions for phylogenetic studies in Angiosperms: The tortoise and the hare III. American Journal of Botany 94: 275-288.
Susanna, A. & Garcia-Jacas, N. 2007. Tribe Cardueae. pp: 123–146 In Kadereit, J.W. & Jeffrey, C. (eds.). The families and genera of vascular plants, vol. 8. Flowering plants: Eudicots; Asterales. Berlin, Heidelberg: Springer.
Swofford, D.L. 2002. PAUP*: PAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4.0b10. Sunderland, Sinauer Associates. [Accessed 11 November 2019].
Trifinopoulos, J., Nguyen, L.T., Haseler, A.V. & Quang Minh, B. 2016. W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research. 44: 232-235. Available from: http://iqtree.cibiv.univie.ac.at [Accessed 11 November 2019].
Tscherneva, O.V. 1962. Cousinia Cass. In: Shishkin, B.K. (ed.), Flora U.S.S.R. vol. 27. Akademiya Nauk, Leningrad; pp: 108-357.
White, J.T., Bruns, T.D., Lee, S.T. & Taylor, J.W. 1990. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. In Innis DH (ed) PCR protocols: a guide to methods and applications. Academic Press, San Diego 31: 315-322.
Tscherneva, O.V., Joharchi, M.R., & Ghahremaninejad, F. 2005. A new species of the genus Cousinia (Asteraceae) from Iran. Botanicheskii Zhurnal 90(3): 411-414
Zare, M., Khossravi, A.R. & Joharchi, M.R. 2013. Distribution patterns of the genus Cousinia (Asteraceae) in Iran. Iranian Journal of Botany 19: 127-141.