تبارزایی مولکولی و آرایه‌شناسی گونه‌های بخشه Stenocephalae از سرده Cousinia (تیره کاسنیان) در شمال‌شرقی ایران

نویسندگان

1 گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت‌مدرس، تهران، ایران

2 گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت‌مدرس، تهران، ایران؛ قطب تبارزایی موجودات زنده ایران، دانشگاه تهران، تهران، ایران

3 هرباریوم مرکزی دانشگاه تهران، دانشکده زیست‌شناسی، پردیس علوم، دانشگاه تهران، تهران، ایران؛ قطب تبارزایی موجودات زنده ایران، دانشگاه تهران، تهران، ایران

4 موسسه گیاهشناسی بارسلونا، بارسلونا، اسپانیا

چکیده
تبار­زایی و تنوع ریختی گونه‌های Cousinia hypopolia، C. decipiens، C. esfandiarii، C. renominota، C. stahliana و C. daryoushiana از بخشه Stenocephalae از سرده Cousinia که در مناطق شمال­شرقی ایران پراکنش دارند، مورد بررسی قرار گرفتند. توالی‌های هسته‌ای ETS , ITS و کلروپلاستی rpl32-trnL(UAG) برای 12 گونه (17 نمونه) تولید شد. روابط تبار­زایشی بین گونه‌ای با استفاده از روش استنباط بیزی و روش بیشینه درست نمایی بازسازی شد. همچنین نمونه‌ها از لحاظ صفات ریخت­شناسی مورد بررسی قرار گرفتند. در این مطالعه جدایی این گونه‌ها توسط نتایج حاصل از داده‌های مولکولی (داده‌های ترکیبی هسته‌ای ETS ,ITS و کلروپلاستی rpl32-trnL(UAG)) علی­رغم دارا بودن صفات ریختی و توزیع جغرافیایی بسیار مشابه تأیید شد. بر اساس مطالعه حاضر گونه‌های ذکر شده، بر اساس صفات ریختی و نتایج حاصل از داده‌های مولکولی از یکدیگر جدا می‌شوند. کلید شناسایی و شرح گونه‌ها ارائه می‌شود. این نتایج با مطالعات قبلی ریخت­شناسی، گرده­شناسی و تشریحی هم­خوانی قابل توجهی نداشت.

کلیدواژه‌ها


عنوان مقاله English

Molecular phylogeny and taxonomy of species of Cousinia section Stenocephalae (Asteraceae) in NE of Iran

نویسندگان English

Simin Kalouti 1
Shahrokh Kazempour-Osaloo 2
Farideh Attar 3
Alfonso Susanna 4
Núria Garcia-Jacas 4
1 Tarbiat Modares University
2 Tarbiat Modares University
3 University of Tehran
4 Botanic Institute of Barcelona (CSIC-ICUB)
چکیده English

In this study, the phylogeny and morphological variation of 6 species of the genus Cousinia sect. Stenocephalae, i.e., C. hypopolia, C. decipiens, C. esfandiarii, C. renominata, C. stahliana and C. daryoushiana, distributed in NE regions of Iran, were investigated. ITS, ETS and rpl32-trnL (UAG) sequences for 12 species (17 specimens) were generated. Phylogenetic interspecies relationships were constructed by Bayesian method and likelihood analysis. The specimens were also investigated in terms of morphological characters. Despite very similar morphological characters and geographical distributions, the separation of these species was confirmed by the obtained results of the molecular data (combined data of plastid and nuclear sequences). On the basis of the present study, the above-mentioned species were almost separated from each other by morphological characters and molecular data. A diagnostic key and the description of these species were also presented. These results did not conform with the previous palynological and anatomical studies of the species.

کلیدواژه‌ها English

Bayesian analysis
chloroplast sequences
geographical distribution
morphological characters
nuclear sequences
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405