تأثیر نیتریک‌اکساید بر پاسخ های آنتی اکسیداتیو اسپندک (تیرۀ قیچیان) در محیط شور

نویسندگان

دانشگاه شهید مدنی آذربایجان

چکیده
در سال­های اخیر، دخالت نیتریک اکساید در فرایندهای مختلف گیاهی از جمله تقلیل اثر مخرب تنش ­ها مشخص شده است. در محیط شور گیاهان با تنش ثانویه اکسیداتیو مواجه می­ شوند. این آزمایش برای ارزیابی تأثیر کاربرد خارجی سدیم نیترو پروساید (SNP) به ­منزلۀ دهندۀ نیتریک اکساید (NO) در تقلیل اثر اکسیداتیو شوری روی اسپندک (.Zygophyllum fabago L) طراحی شد. SNP (200 میکرومولار) به محیط کشت گیاهان در وضعیت شور (200 و 400 میلی­مولار NaCl) و غیر شور اضافه شد. رشد، نشانگرهای تنش اکسیداتیو (شاخص پایداری غشای سلولی و غلظت پراکسید هیدروژن)، فعالیت آنزیم ­های آنتی­ اکسیدان (پراکسیداز و کاتالاز) و همین­طور محتوای برخی ترکیبات آنتی­ اکسیدان (فلاونوییدها و کاروتنوییدها) تعیین شدند. شوری موجب کاهش معنی­دار وزن خشک اندام­ های هوایی و ریشه شد ولی فعالیت آنزیم­های پراکسیداز و کاتالاز را افزایش داد. شوری زیاد موجب افزایش محتوای پراکسید هیدروژن و کاهش میزان کاروتنوییدها در برگ­ها شد. کاربرد برون­زای NO ­در محیط شور موجب افزایش رشد، شاخص پایداری غشا و محتوای فلاونوییدها و کاروتنوییدها شد. کاربرد SNP در محیط شور موجب کاهش معنی­ دار پراکسید هیدروژن و همین طور فعالیت آنزیم ­های آنتی ­اکسیدان تحت مطالعه در برگ­ ها شد. داده­ ها حاکی از آن هستند که همکاری سیستم ­های آنتی اکسیدان اسپندک برای فایق آمدن بر شوری تأثیر می ­گذارد. همچنین، از داده­ ها بر­می­ آید که کاربرد خارجی NO برای تقلیل اثر اکسیداتیو شوری در گیاه اسپندک مفید است.

کلیدواژه‌ها


عنوان مقاله English

Effect of nitric oxide on antioxidative responses under salinity conditions in Zygophyllum fabago L. (Zygophyllaceae)

نویسندگان English

Nader Chaparzadeh
Roya Saeedifar
Leila Zarandi-Miandoab
Mohammad Pazhang
Azarbaijan Shahid Madani University
چکیده English

In recent years, the involvement of nitric oxide (NO) in numerous physiological processes, particularly the mitigation of stress-induced negative effects on plants, has been clarified. Under salinity conditions, plants are subjected to a secondary oxidative stress. The present work was designed to examine the exogenous application of nitric oxide (NO), in the form of its donor sodium nitroprusside (SNP), in mitigating the deleterious effects of salinity on Zygoph-yllum fabago L. plants. SNP (200 µM) was applied to plants growing medium under saline (200 and 400 mM NaCl) and non-saline conditions. Growth, oxidative stress markers [cell membrane stability index (MSI) and H2O2 conc-entration], antioxidant enzymes activities [peroxidase (POX, EC 1.11.1.7) and catalase (CAT, EC 1.11.1.6)], as well as the contents of some antioxidant compounds (flavonoids and carotenoids) were determined. Salinity lowered the shoot and root dry weights, while it enhanced peroxidase and catalase activities. High salinity increased H2O2; however, it de-creased the carotenoids content of leaves. Exogenous NO enhanced the growth, MSI, flavonoids and carotenoids co-ntents of salinized plants. In salinity plus SNP treated plants, H2O2 concentration and the activities of the examined en-zymes were reduced. Data suggest that a cooperative process is performed by the antioxidant systems in Syrian bean ca-per in order to cope with salinity. Also, the application of exogenous NO was found to be useful in the mitigation of salinity-induced oxidative stress in plants.

کلیدواژه‌ها English

Peroxidase
hydrogen peroxide
sodium nitroprusside
NaCl
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دوره 4، شماره 2 - شماره پیاپی 12
تابستان 1396
صفحه 155-165

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405