Effect of nitric oxide on antioxidative responses under salinity conditions in Zygophyllum fabago L. (Zygophyllaceae)

Chaparzadeh, Nader; Saeedifar, Roya; Zarandi-Miandoab, Leila; Pazhang, Mohammad

doi:10.21859/acadpub.nbr.4.2.155

Effect of nitric oxide on antioxidative responses under salinity conditions in Zygophyllum fabago L. (Zygophyllaceae)

Authors

Nader Chaparzadeh

Roya Saeedifar

Leila Zarandi-Miandoab

Mohammad Pazhang

Azarbaijan Shahid Madani University

https://doi.org/10.21859/acadpub.nbr.4.2.155

Abstract

In recent years, the involvement of nitric oxide (NO) in numerous physiological processes, particularly the mitigation of stress-induced negative effects on plants, has been clarified. Under salinity conditions, plants are subjected to a secondary oxidative stress. The present work was designed to examine the exogenous application of nitric oxide (NO), in the form of its donor sodium nitroprusside (SNP), in mitigating the deleterious effects of salinity on Zygoph-yllum fabago L. plants. SNP (200 µM) was applied to plants growing medium under saline (200 and 400 mM NaCl) and non-saline conditions. Growth, oxidative stress markers [cell membrane stability index (MSI) and H₂O₂ conc-entration], antioxidant enzymes activities [peroxidase (POX, EC 1.11.1.7) and catalase (CAT, EC 1.11.1.6)], as well as the contents of some antioxidant compounds (flavonoids and carotenoids) were determined. Salinity lowered the shoot and root dry weights, while it enhanced peroxidase and catalase activities. High salinity increased H₂O₂; however, it de-creased the carotenoids content of leaves. Exogenous NO enhanced the growth, MSI, flavonoids and carotenoids co-ntents of salinized plants. In salinity plus SNP treated plants, H₂O₂ concentration and the activities of the examined en-zymes were reduced. Data suggest that a cooperative process is performed by the antioxidant systems in Syrian bean ca-per in order to cope with salinity. Also, the application of exogenous NO was found to be useful in the mitigation of salinity-induced oxidative stress in plants.

Keywords

Peroxidase

hydrogen peroxide

sodium nitroprusside

NaCl

20.1001.1.24236330.1396.4.2.5.8

Ahmad, P., Sarwat, M. and Sharma, S. 2008. Reactive oxygen species, antioxidants and signaling in plants. – J. Plant Biol. 51:167-173.
Ahmad. P., Abdel Latef, A.A., Hashem, A., Abd Allah, E.F., Gucel, S. and Tran, L.S. 2016. Nitric oxide miti-gates salt stress by regulating levels of osmolytes and antioxidant enzymes in chickpea. – Front. Plant Sci. 7: 347-358.
Barcelo, A.R., Pomar, F., Lopez-Serrano, M. and Pedr-eno, M.A. 2003. Peroxidase: a multifunctional enzy-me in grapevines. – Funct. Plant Biol. 30: 577-591.
Beligni, M.V. and Lamattina, L. 1999. Nitric oxide co-unteracts cytotoxic processes mediated by reactive oxygen species in plant tissues. – Planta 208: 337-344.
Bradford, M. 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. – Anal. Biochem. 72: 248-254.
Bromham, L. 2014. Macroevolutionary patterns of salt tolerance in angiosperms. – Ann. Bot. 115: 333-341.
Chaparzadeh, N.D. Amico, M.L., Khavari-Najad, R.A., Izzo, R. and Navari-Izzo, F. 2004. Antioxidative re-sponses of Calendula officinalis under salinity cond-itions. – Plant. Physiol. Biochem. 42: 695-701.
Chaparzadeh, N., and Zarandi-Miandoab, L. 2011. The effects of salinity on pigments content and growth of two canola (Brassica napus) cultivars. – IJPB 9: 13-26.
Corpas, F.J., Leterrier, M., Valderrama, R., Airaki, M., Chaki, M., Palma, J.M. and Barroso, J.B. 2011. Nitric oxide imbalance provokes a nitrosative resp-onse in plants under abiotic stress. – Plant Sci. 181: 604-611.
Dong, Y.J., Jinc, S.S., Liu,S., Xu,L.L. and Kong, J. 2014. Effects of exogenous nitric oxide on growth of cotton seedlings under NaCl stress. – J. Soil Sci. Plant Nutr. 14: 1-13.
Esim, N. and Atici, O. 2013. Nitric oxide alleviates boron toxicity by reducing oxidative damage and growth inhibition in maize seedlings (Zea mays L.). – A.J.C.S. 7: 1085-1092.
Esim, N. and Atici, O. 2014. Nitric oxide improves chilling tolerance of maize by affecting apoplastic an-tioxidative enzymes in leaves. – Plant Growth Regul. 72: 29-38.
Flowers, T.J. 2004. Improving crop salt tolerance. – J. Exp. Bot. 55: 307-319.
Hayat, S., Hasan, S.A., Mori, M., Fariduddin, Q. and Ahmad, A. 2009. Nitric oxide: chemistry, biosyn-thesis, and physiological role. In: Hayat, S., Mori, M., Pichtel, J. and Ahmad, A. (ed.), Nitric oxide in plant physiology. Pp: 1-15. Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim.
Jiang, Y. and Huang, B. 2001. Drought and heat stress injury to two cool-season turf grasses in relation to antioxidant metabolism and lipid peroxidation. – Crop Sci. 41: 436-442.
Krizek, D.T., Britz, S.J. and Mirecki, R.M. 1998. Inhi-bitory effects of ambient levels of solar UV‐A and UV‐B radiation on growth of cv. New Red Fire lettuce. – Physiol. Plant. 103: 1-7.
Lamattina, L., Garcia-Mata, C., Graziano, M. and Pagn-ussat, G. 2003. Nitric oxide: the versatility of an ex-tensive signal molecule. – Ann. Rev. Plant Biol. 54: 109-136.
Liu, X., Wang, L., Liu, L., Guo, Y. and Ren, H. 2013. Alleviating effect of exogenous nitric oxide in cucu-mber seedling against chilling stress. – Afr. J. Biot-echnol. 10: 4380-4386.
Manai, J., Kalai T., Gouia H. and Corpas F.J. 2014. Ex-ogenous nitric oxide (NO) ameliorates salinity indu-ced oxidative stress in tomato (Solanum lycopersicum) plants. – J. Soil Sci. Plant Nutr. 14: 433-446.
Mostofa, M.G., Fujita, M. and Tran, LS.P. 2015. Nitric oxide mediates hydrogen peroxide-and salicylic acid induced salt tolerance in rice (Oryza sativa L.) seedlings. – Plant Growth Regul. 77: 265-277.
Nazar, R., Iqbal, N., Syeed, S. and Khan, N.A. 2011. Sa-licylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur as-similation and antioxidant metabolism differentially in two mungbean cultivars. – J. Plant Physiol. 168: 807-815.
Oz, M.T., Eyidogan, F., Yucel, M. and Oktem H.A. 2015. Functional role of nitric oxide under abiotic st-ress conditions. In: Khan, M.N. et al. (ed.), nitric ox-ide action in abiotic stress responses in plants. Pp: 21-41. – Springer International Publishing Switzerland.
Parida, A.K. and Das, A.B. 2005. Salt tolerance and salinity effects on plants: a review. – Ecotox. Envi-ron. Safe. 60: 324-349.
Ruan, C.J., da Silva, J.A.T., Mopper, S., Qin, P. and Lutts, S. 2010. Halophyte improvement for a salini-zed world. – Crit. Rev. Plant Sci. 29: 329-359.
Sairam, R.K., Rao, K.V. and Srivastava G.C. 2002. Differential response of wheat genotypes to long-te-rm salinity stress in relation to oxidative stress, anti-oxidant activity and osmolyte concentration. – Plant Sci. 163: 1037-1046.
Shannon, M. and Grieve, C. 1998. Tolerance of vege-table crops to salinity. – Sci. Hortic. 78: 5-38.
Sheokand, S., Kumari, A. and Sawhney, V. 2008. Effect of nitric oxide and putrescine on antioxidative resp-onses under NaCl stress in chickpea plants. – Physiol. Mol. Biol. Plants 14: 355-362.
Tarchoune, I., Sgherri, C., Izzo, R. Lachaa, M., Navari-Izzo, F. and Ouerghi, Z. 2012. Changes in the an-tioxidative systems of Ocimum basilicum L. (cv. Fine) under different sodium salts. – Acta Physiol. Pl-ant. 34: 1873-1881.
Terzi, R., Kadioglu A., Kalaycioglu, E. and Saglam, A. 2014. Hydrogen peroxide pretreatment induces os-motic stress tolerance by influencing osmolyte and abscisic acid levels in maize leaves. – J. Plant Interact. 9: 559-565
Tian, X. and Lei, Y. 2006. Nitric oxide treatment allevi-ates drought stress in wheat seedlings. – Biol. Plan-tarum 50: 775-778.
Wahid, A., Perveen, M., Gelani, S. and Basra, S. 2007. Pretreatment of seed with H2O2 improves salt tole-rance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. – J. Plant Physiol. 164: 283-294.
Wang, H., Zhang, S., Zhang, W., Wei, C. and Wang, P. 2010. Effects of nitric oxide on the growth and anti-oxidant response of submerged plants Hydrilla vert-icillata (Lf) Royle. – Afr. J. Biotechnol. 9: 7470-7476.
Wang, W., Yan, Z., You, S., Zhang, Y., Chen, L. and Lin, G. 2011. Mangroves: obligate or facultative ha-lophytes? A review. – Trees 25: 953-963.
Wu, X.X., Zhu, W., Zhang, H., Ding, H. and Zhang, H.J. 2011. Exogenous nitric oxide protects against salt-induced oxidative stress in the leaves from two geno-types of tomato (Lycopersicon esculentum Mill.). – Acta Physiol. Plant. 33: 1199-1209.
Zhang, Y., Wang, L., Liu, Y., Zhang, Q., Wei, Q. and Zhang, W. 2006. Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of pr-oton-pump and Na+/H+ antiport in the tonoplast. – Pl-anta 224: 545-555.
Zhang, Y. and Liu Y. 2004. Source and function of nitric oxide in plants. – Acta Bot. Boreal Occident Sin. 24: 921-929.
Zheng, C., Dong, J., Liu, F., Dai, T., Liu, W., Jing, Q. and Cao, W. 2009. Exogenous nitric oxide improves seed germination in wheat against mitochondrial ox-idative damage induced by high salinity. – Environ. Exp. Bot. 67: 222-227.