تأثیر سطوح مختلف آهن فریرون و پروبیوتیک بیوپلاس ب-2 بر برخی پارامترهای خونی بچه‌ماهی سفید (Rutilus frisii kutum)

نویسندگان

دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده
در تحقیق حاضر تأثیر ترکیب پروبیوتیک (بیوپلاس ب-2) و آهن (فریرون) بر برخی از پارامترهای خونی 270 بچه­ماهی سفید (Rutilus frisii kutum) با میانگین وزنی 1/0±4/0 گرم و میانگین طولی 3/0±9/3 سانتی­متر در 9 تیمار با سه تکرار در مدت 60 روز تحت بررسی قرار گرفت. نتایج نشان داد که حداکثر تعداد گلبول­های قرمز 55/1±106×65/1 عدد در میلی­متر مکعب، میزان هموگلوبین 15/0±63/7 گرم در دسی­لیتر خون، هماتوکریت 85/0­±­03/24 درصد، MCV 48/1 ± 45/1 فمتولیتر، MCH 25/0±29/46 پیکوگرم و لنفوسیت 2±92 درصد مربوط به تیمار 5 بود. بیشترین میزان MCHC (67/0±65/34 گرم در دسی لیتر) در تیمار 4 و بالاترین تعداد گلبول­های سفید (100±6/6 عدد در میلی­متر مکعب) در تیمار 8 مشاهده شد. یافته­های این تحقیق نشان می­دهد تیمار 5 که حاوی بالاترین میزان آهن (7 میلی­گرم در هر کیلوگرم غذا) و پروبیوتیک (CFC 109×­6/1) در غذا بود بالاترین تعداد گلبول­های قرمز، هموگلوبین، هماتوکریت، MCV، MCH و لنفوسیت را در این پژوهش نشان داد.

کلیدواژه‌ها


عنوان مقاله English

The effect of different levels of Fe (So4)2 (7H2O) and probiotics (BioPlus-2B) on some blood parameters of Rutilus frisii kutum fry

نویسندگان English

Hajar Azari
Mohammadreza Imanpour
Mina Rajabpour
Noushin Mahdinejad
Ali Jafer Noude
Gorgan University of Agricultural Sciences and Natural Resources,
چکیده English

In this study the effect of iron of Fe (So4)2 (7H2O( and probiotics BioPlus 2B on some blood parameters was investigated on 270 of Rutilus frisii kutum fry with total weight of 0.4±0.1 gr and total length of 3.9±0.3 cm in nine treatments and triplicates during 60 days. Results showed that the highest number of red blood cells (RBCs) (1.65×106± 1.55 per mm3), hemoglobin concentration (7.36±0.15 g/dl), hematocrit concentration (24.03±0.85 %), MCV (1.45±1.48 Fl), MCH (46.29±0.25 pg) and lymphocyte (92±2) belong to treatment 5. The highest level of MCHC (34.65±0.67 g/dl) and the greatest level of white blood cells (6.6±100 per mm3) were observed in treatments 4 and 8, respectively. The findings of this research showed that treatment 5 contained the highest amount of iron (7 mg/kg of feed) and probiotics (CFC 1.6×109) resulted in the highest amount of red blood cells, hemoglobin, hematocrit, MCV, MCH and lymphocytes in this study.

کلیدواژه‌ها English

Rutilus frisii kutum
blood factors
probiotic
BioPlus 2B
.و قرایی، ا قلیچی، ا ر.،اکرمی، ها در آبزی. کاربرد پروبیوتیک1389- . پروری – : 4علمی پژوهشی دانشگاه آزاد اسلامی واحد آزادشهر ۀمجل - 84- 77.
.طاعتی کلی، م و نظرپور، ع .،جعفریان، ح بررسی. 1388 اثر باسیل های بیوتیکیپرو بر رشد لارو ماهی آلایقزل کمان رنگین (mykiss Oncorhynchus (از طریق مکمل سازی با آرد دافنی ماگنا (magna Daphnia. – .(48-59: 3علوم کشاورزی و منابع طبیعی ۀ مجل

.فروزانفر، ع و شکوری، م .،خارا، ح .،نظامی، ش .،ناصری، س . 1387 بر برخی فاکتورهای خونی آلوین ک و آهن تاثیر سطوح مختلف پروبیوتی کمان (آلای رنگینلقز mykiss Oncorhynchus – (. علوم زیستی ۀمجل لاهیجان واحد 2: 73-81.
Arianto, A. and Austin, B. 2002. Probiotics in aquaculture: Review. – Journal of Fish Diseases 25: 633-642.
Bagheri, T., Hedayati, S.A., Yavari, V., Alizade, M. and Farzanfar, A. 2008. Growth, survival and gut microbial load of rainbow trout (Onchorhynchus mykiss) fry given diet supplemented with probiotic during the two months of first feeding. – Turkish Journal of Fisheries and Aquatic Sciences 8: 43-48.
Benfey, T.J. and Sutterlin, A.M. 1984. The haematology of triploid landlocked Atlantic salmon, salmo salar, L. – J. Fish Biol. 24: 333-338. Blaxhall, P.C. 1972. The haematological assessment of the health of fresh water fish. – Journal of Fish Biol. 4: 593-604.
Chang, C.I.W and Liu, W.Y. 2002. An evaluation of two bacterial strains, Enterococcus faecium SF68 and Bacillus toyoi, for reducing edwardsiellosis in cultured European eel, Anguilla Anguilla L. – Journal of Fish Diseases 25: 311-315.
Choi S.H. and Yoon, T.J. 2008. Non-specific immune response of rainbow trout (Oncorhynchus Mykiss) by dietary heat-inactivated potential probiotics. – Immune Net. 8: 67-74.
Farzanfar, A., Lashto Aghaei, G., Alizadeh, M., Bayati, M. and Ghorban, R. 2007. Study on growth performance of Rainbow trout, Oncorhynchus mykiss, larvae with different concentration of probiotic in diet. – In: proceedings of Aquaculture, San Antonio, Texas, USA.
Farzanfar, A. 2006. The use of probiotics in shrimp aquaculture. – FEMS Immunology and Medical Microbiology 48: 149-158.
Firdaus, S., Jafri, A.K. and Rahman, N. 1994. Effects of iron-deficient diet on the growth and haematological characteristics of the catfish Heteropueustes fossilis Bloch. – Journal of Aquaculture in the Tropics 9: 179-185.
Gatlin, D.M. 2002. Nutrition and Fish Health. Fish Nutrition. – Academic Press, San Diego, CA, USA. 671 pp.
Ghosh, S., Sinha, A. and Sahu, C. 2008. Dietary probiotic supplementation in growth and health of livebearing ornamental fishes. – Aquaculture Nutr. 14: 289-299.
Gomez-Gil, B., Roque, A. and Turnbull, J.F. 2000. The use selection of probiotic bacteria for use in the culture of larval aquatic organisms. – Aquaculture 9: 259-270.
Gullian, M., Thompson, F. and Rodriguez, J. 2004. Selection of probiotic bacteria and study of their immunostimulatory effect in Penaeus vannamei. – Aquaculture 233: 1-14.
Houston, A.H. 1990. Blood and Circulation. Methods for Fish Biology. – American Fisheries Society, Bethesda, Maryland 9: 273-334.
Karimi, K. and Rahimi, S.H. 2004. The effects of different levels of probiotic on levels of fats and red cells of broilers. – J. Pejuohesh and Sazandeghi 62: 40-45.
Kurti, P. 2000. Microbial balance and optimal digestion in pigs. – International Pig Topics 16: 17-19.
Mehrim, A.I. 2009. Effect of dietary supplementation of Biogen (Commercial probiotic) on mono-sex Nile tilapia (Oreochromis niloticu) sunder differentstocking densities. – J. Fisher Aquatic Science 4: 261-273.
Mohan, B.R., Kadirvel, S.K., Natarajan, R. and Bhaskaran, M. 1996. Effect of probiotic supplementation on growth nitrogen utilization and serum cholestrol in broilers. – British Poultry Science 37: 395- 401.
Moriarty, D.J.W. 1998. Control of luminous vibrio species in Penaeid aquaculture ponds. Aquaculture 164: 351-358.
Nayak, S.K. 2010. Probiotics and immunity: A fish perspective. – Fish and Shellfish Immunology 29: 2-14.
Olafsen, J.A. 1998. Interactions between hosts and bacteria in aquaculture. – In Proceedings from the USEC Workshop on Marine Microorganisms: Research Issues for Biotechnology. European Commission, Brussels, Belgium.
Panigrahi, A., Kiron, V., Puangkaew, J., Kobayashi, T., Satoh, S. and Sugita, H. 2005. The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss. – Aquaculture 243: 241-254.
Panigrahi, A., Kiron, V., Kobayashi, T., Puangkaew, J., Satoh, S. and Sugita, H. 2004. Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus Rhamnosus JCM 1136. – Immunol. Immunopathol. 102: 379-388.
Reid, R.T., Live, D.H., Faulkner, D.J. and Butler, A. 1993. A siderophore from a marine bacterium with an exceptional ferric ion affinity constant. – Nature 366: 455-458.
Serrano, P.H. 2005. Responsible use of antibiotics in aquaculture. – FAO Fisheries Technical Paper. 97 pp.
Schiffrin, E.J., Brassart, D., Servin, A.L., Rochat, F. and Donnet-Hughes, A. 1997. Immune modulation of blood leucocytes in humans by lactic acid bacteria: Criteria for strain selection. American Journal of Clinical Nutrition 2: 515-520.
Song, Z., Wu, T., Cai, L. Zhang, L. and Zheng, X. 2006. Effects of dietary supplementation with Clostridium butyricumon the growth performance and humoral immune response in Miichthys miiuy. – J. Zhejiang Univ. Sci. 7: 596-602.
Sharifuzzaman, S.M. and Austin, B. 2009. Influence of probiotic feeding duration on disease resistance and immune parameters in rainbow trout. – Fish Shellfish Immunol. 27: 440-445.
Wooldridge, K.G. and Williams, P.H. 1993. Iron uptake mechanisms of pathogenic bacteria. – FEMS Microbiology Reviews 12: 325-348.
Yanbo, W. and Zirong, X. 2006. Effect of probiotic for commom carp (Cyprinus carpio) based on growth performance and digestive enzymes activities. – Journal of Animal Feed Science and Technology 127: 283-292.

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405