The effect of different levels of Fe (So4)2 (7H2O) and probiotics (BioPlus-2B) on some blood parameters of Rutilus frisii kutum fry

Azari, Hajar; Imanpour, Mohammadreza; Rajabpour, Mina; Mahdinejad, Noushin; Jafer Noude, Ali

doi:10.21859/acadpub.nbr.2.2.82

The effect of different levels of Fe (So4)2 (7H2O) and probiotics (BioPlus-2B) on some blood parameters of Rutilus frisii kutum fry

Authors

Hajar Azari

Mohammadreza Imanpour

Mina Rajabpour

Noushin Mahdinejad

Ali Jafer Noude

Gorgan University of Agricultural Sciences and Natural Resources,

https://doi.org/10.21859/acadpub.nbr.2.2.82

Abstract

In this study the effect of iron of Fe (So₄)₂ (7H₂O( and probiotics BioPlus 2B on some blood parameters was investigated on 270 of Rutilus frisii kutum fry with total weight of 0.4±0.1 gr and total length of 3.9±0.3 cm in nine treatments and triplicates during 60 days. Results showed that the highest number of red blood cells (RBCs) (1.65×10⁶± 1.55 per mm³⁾, hemoglobin concentration (7.36±0.15 g/dl), hematocrit concentration (24.03±0.85 %), MCV (1.45±1.48 Fl), MCH (46.29±0.25 pg) and lymphocyte (92±2) belong to treatment 5. The highest level of MCHC (34.65±0.67 g/dl) and the greatest level of white blood cells (6.6±100 per mm³) were observed in treatments 4 and 8, respectively. The findings of this research showed that treatment 5 contained the highest amount of iron (7 mg/kg of feed) and probiotics (CFC 1.6×10⁹) resulted in the highest amount of red blood cells, hemoglobin, hematocrit, MCV, MCH and lymphocytes in this study.

Keywords

Rutilus frisii kutum

blood factors

probiotic

BioPlus 2B

20.1001.1.24236330.1394.2.2.1.0

.و قرایی، ا قلیچی، ا ر.،اکرمی، ها در آبزی. کاربرد پروبیوتیک1389- . پروری – : 4علمی پژوهشی دانشگاه آزاد اسلامی واحد آزادشهر ۀمجل - 84- 77.
.طاعتی کلی، م و نظرپور، ع .،جعفریان، ح بررسی. 1388 اثر باسیل های بیوتیکیپرو بر رشد لارو ماهی آلایقزل کمان رنگین (mykiss Oncorhynchus (از طریق مکمل سازی با آرد دافنی ماگنا (magna Daphnia. – .(48-59: 3علوم کشاورزی و منابع طبیعی ۀ مجل

.فروزانفر، ع و شکوری، م .،خارا، ح .،نظامی، ش .،ناصری، س . 1387 بر برخی فاکتورهای خونی آلوین ک و آهن تاثیر سطوح مختلف پروبیوتی کمان (آلای رنگینلقز mykiss Oncorhynchus – (. علوم زیستی ۀمجل لاهیجان واحد 2: 73-81.
Arianto, A. and Austin, B. 2002. Probiotics in aquaculture: Review. – Journal of Fish Diseases 25: 633-642.
Bagheri, T., Hedayati, S.A., Yavari, V., Alizade, M. and Farzanfar, A. 2008. Growth, survival and gut microbial load of rainbow trout (Onchorhynchus mykiss) fry given diet supplemented with probiotic during the two months of first feeding. – Turkish Journal of Fisheries and Aquatic Sciences 8: 43-48.
Benfey, T.J. and Sutterlin, A.M. 1984. The haematology of triploid landlocked Atlantic salmon, salmo salar, L. – J. Fish Biol. 24: 333-338. Blaxhall, P.C. 1972. The haematological assessment of the health of fresh water fish. – Journal of Fish Biol. 4: 593-604.
Chang, C.I.W and Liu, W.Y. 2002. An evaluation of two bacterial strains, Enterococcus faecium SF68 and Bacillus toyoi, for reducing edwardsiellosis in cultured European eel, Anguilla Anguilla L. – Journal of Fish Diseases 25: 311-315.
Choi S.H. and Yoon, T.J. 2008. Non-specific immune response of rainbow trout (Oncorhynchus Mykiss) by dietary heat-inactivated potential probiotics. – Immune Net. 8: 67-74.
Farzanfar, A., Lashto Aghaei, G., Alizadeh, M., Bayati, M. and Ghorban, R. 2007. Study on growth performance of Rainbow trout, Oncorhynchus mykiss, larvae with different concentration of probiotic in diet. – In: proceedings of Aquaculture, San Antonio, Texas, USA.
Farzanfar, A. 2006. The use of probiotics in shrimp aquaculture. – FEMS Immunology and Medical Microbiology 48: 149-158.
Firdaus, S., Jafri, A.K. and Rahman, N. 1994. Effects of iron-deficient diet on the growth and haematological characteristics of the catfish Heteropueustes fossilis Bloch. – Journal of Aquaculture in the Tropics 9: 179-185.
Gatlin, D.M. 2002. Nutrition and Fish Health. Fish Nutrition. – Academic Press, San Diego, CA, USA. 671 pp.
Ghosh, S., Sinha, A. and Sahu, C. 2008. Dietary probiotic supplementation in growth and health of livebearing ornamental fishes. – Aquaculture Nutr. 14: 289-299.
Gomez-Gil, B., Roque, A. and Turnbull, J.F. 2000. The use selection of probiotic bacteria for use in the culture of larval aquatic organisms. – Aquaculture 9: 259-270.
Gullian, M., Thompson, F. and Rodriguez, J. 2004. Selection of probiotic bacteria and study of their immunostimulatory effect in Penaeus vannamei. – Aquaculture 233: 1-14.
Houston, A.H. 1990. Blood and Circulation. Methods for Fish Biology. – American Fisheries Society, Bethesda, Maryland 9: 273-334.
Karimi, K. and Rahimi, S.H. 2004. The effects of different levels of probiotic on levels of fats and red cells of broilers. – J. Pejuohesh and Sazandeghi 62: 40-45.
Kurti, P. 2000. Microbial balance and optimal digestion in pigs. – International Pig Topics 16: 17-19.
Mehrim, A.I. 2009. Effect of dietary supplementation of Biogen (Commercial probiotic) on mono-sex Nile tilapia (Oreochromis niloticu) sunder differentstocking densities. – J. Fisher Aquatic Science 4: 261-273.
Mohan, B.R., Kadirvel, S.K., Natarajan, R. and Bhaskaran, M. 1996. Effect of probiotic supplementation on growth nitrogen utilization and serum cholestrol in broilers. – British Poultry Science 37: 395- 401.
Moriarty, D.J.W. 1998. Control of luminous vibrio species in Penaeid aquaculture ponds. Aquaculture 164: 351-358.
Nayak, S.K. 2010. Probiotics and immunity: A fish perspective. – Fish and Shellfish Immunology 29: 2-14.
Olafsen, J.A. 1998. Interactions between hosts and bacteria in aquaculture. – In Proceedings from the USEC Workshop on Marine Microorganisms: Research Issues for Biotechnology. European Commission, Brussels, Belgium.
Panigrahi, A., Kiron, V., Puangkaew, J., Kobayashi, T., Satoh, S. and Sugita, H. 2005. The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss. – Aquaculture 243: 241-254.
Panigrahi, A., Kiron, V., Kobayashi, T., Puangkaew, J., Satoh, S. and Sugita, H. 2004. Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus Rhamnosus JCM 1136. – Immunol. Immunopathol. 102: 379-388.
Reid, R.T., Live, D.H., Faulkner, D.J. and Butler, A. 1993. A siderophore from a marine bacterium with an exceptional ferric ion affinity constant. – Nature 366: 455-458.
Serrano, P.H. 2005. Responsible use of antibiotics in aquaculture. – FAO Fisheries Technical Paper. 97 pp.
Schiffrin, E.J., Brassart, D., Servin, A.L., Rochat, F. and Donnet-Hughes, A. 1997. Immune modulation of blood leucocytes in humans by lactic acid bacteria: Criteria for strain selection. American Journal of Clinical Nutrition 2: 515-520.
Song, Z., Wu, T., Cai, L. Zhang, L. and Zheng, X. 2006. Effects of dietary supplementation with Clostridium butyricumon the growth performance and humoral immune response in Miichthys miiuy. – J. Zhejiang Univ. Sci. 7: 596-602.
Sharifuzzaman, S.M. and Austin, B. 2009. Influence of probiotic feeding duration on disease resistance and immune parameters in rainbow trout. – Fish Shellfish Immunol. 27: 440-445.
Wooldridge, K.G. and Williams, P.H. 1993. Iron uptake mechanisms of pathogenic bacteria. – FEMS Microbiology Reviews 12: 325-348.
Yanbo, W. and Zirong, X. 2006. Effect of probiotic for commom carp (Cyprinus carpio) based on growth performance and digestive enzymes activities. – Journal of Animal Feed Science and Technology 127: 283-292.