Volume 6, Issue 1 (5-2019)                   nbr 2019, 6(1): 10-19 | Back to browse issues page


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Meimandi K, Yaghoobi M M. The effect of aqueous and ethanolic extracts of Sedum album L. on human stomach and breast carcinoma cell lines in vitro. nbr 2019; 6 (1) :10-19
URL: http://nbr.khu.ac.ir/article-1-2897-en.html
Graduate University of Advanced Technology , m.yaghoobi@kgut.ac.ir
Abstract:   (5308 Views)

In this study, the cytotoxic effects of aqueous and ethanolic extracts of Sedum album L. on human stomach cancer cell line (AGS) and breast cancer cell line (MCF-7) were evaluated by MTT, BrdU and TUNEL assays. The results demonstrated that both extracts had antiproliferative and apoptotic effects in a dose-dependent manner. The MTT assay data revealed that the AGS cell underwent more cytotoxicity in comparison with the MCF-7 cell. It also revealed that ethanolic extract was more potent than aqueous extract. The BrdU assay results showed that the proliferation of AGS and MCF-7 cells was reduced to 50% and 43%, respectively, at the highest concentration of the aqueous extract. In addition, the ethanolic extract reduced the proliferation of AGS and MCF-7 cells to 75% and 60%, respectively. The AGS and MCF-7 cells underwent 52% and 12% apoptotic death upon treatment by the ethanolic extract as TUNEL assay showed. The aqueous extract induced 28% and 25% apoptosis in the AGS and MCF-7 cells, respectively. Both inhibition of proliferation and induction of apoptosis are desirable strategies for cancer treatment among researchers. Identification of S. album compounds and analyzing their effects in animal model of cancer can help us with understanding its anti-cancer properties.
 

 

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Type of Study: Original Article | Subject: Cell and Molecular Biology
Received: 2017/07/9 | Revised: 2019/05/5 | Accepted: 2017/12/4 | Published: 2019/04/30 | ePublished: 2019/04/30

References
1. Atanasov, A.G., Waltenberger, B., Pferschy-Wenzig, E.M., Linder, T., Wawrosch, C., Uhrin, P., Temml, V., Wang, L., Schwaiger, S., Heiss, E.H., Rollinger, J.M., Schuster, D., Breuss, J.M., Bochkov, V., Mihovilovic, M.D., Kopp, B., Bauer, R., Dirsch, V.M. and Stuppner, H. 2015. Discovery and resupply of pharmacologically active plant-derived natural products: A review. - Biotechnol Adv. 33: 1582-1614. [DOI:10.1016/j.biotechadv.2015.08.001]
2. Bai, Y., Chen, B., Hong, W., Liang, Y., Zhou, M. and Zhou, L. 2016. Sedum sarmentosum bunge extract induces apoptosis and inhibits proliferation in pancreatic cancer cells via the hedgehog signaling pathway. - Oncol. Rep. 35: 2775-2784. [DOI:10.3892/or.2016.4679]
3. Barranco, S., Townsend, C., Casartelli, C., Macik, B., Broerwinkle, W. and Gourley, W. 1983. Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. - Cancer Res. 43: 1703-1709.
4. Huang, D., Zhang, W., Huang, D. and Wu, J. 2010. Antitumor activity of the aqueous extract from sedum sarmentosum bunge in vitro. - Cancer Biother. Radiopharm. 25: 81-88. [DOI:10.1089/cbr.2009.0632]
5. Jung, H. J., Kang, H. J., Song, Y. S., Park, E. H., Kim, Y. M. and Lim, C. J. 2008. Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of sedum sarmentosum extract. - J. Ethnopharmacol. 116: 138-143. [DOI:10.1016/j.jep.2007.11.014]
6. Kang, T. H., Pae, H.O., Yoo, J. C., Kim, N.Y., Kim, Y. C., Ko, G.I. and Chung, H.T. 2000. Antiproliferative effects of alkaloids from sedum sarmentosum on murine and human hepatoma cell lines. - J. Ethnopharmacol. 70: 177-182. [DOI:10.1016/S0378-8741(99)00164-6]
7. Kashyap, D., Mittal, S., Sak, K., Singhal, P. and Tuli, H. S. 2016. Molecular mechanisms of action of quercetin in cancer: recent advances. - Tumour Biol. 37: 12927-12939. [DOI:10.1007/s13277-016-5184-x]
8. Ma, G., Yang, C., Qu, Y., Wei, H., Zhang, T. and Zhang, N. 2007. The flavonoid component isorhamnetin in vitro inhibits proliferation and induces apoptosis in eca-109 cells. - Chem. Biol. Interact. 167: 153-160. [DOI:10.1016/j.cbi.2007.02.006]
9. Ma, X., Yang, J., Deng, S., Huang, M., Zheng, S., Xu, S., Cai, J., Yang, X. and Ai, H. 2017. Two new megastigmanes from chinese traditional medicinal plant sedum sarmentosum. - Nat. Prod. Res. 31: 1473-1477. [DOI:10.1080/14786419.2017.1280493]
10. Meacham, C.E. and Morrison, S.J. 2013. Tumor heterogeneity and cancer cell plasticity. - Nature 501: 328-337. [DOI:10.1038/nature12624]
11. Nakatsu, N., Yoshida, Y., Yamazaki, K., Nakamura, T., Dan, S., Fukui, Y. and Yamori, T. 2005. Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cdna arrays. - Mol. Cancer Ther. 4: 399-412.
12. Newman, D. J. and Cragg, G. M. 2016. Natural products as sources of new drugs from 1981 to 2014. - J. Nat. Prod. 79: 629-661. [DOI:10.1021/acs.jnatprod.5b01055]
13. Ouyang, L ., Luo, Y., Tian, M., Zhang, S. Y., Lu, R., Wang, J. H., Kasimu, R. and Li, X. 2014. Plant natural products: from traditional compounds to new emerging drugs in cancer therapy. - Cell Prolif. 47: 506-515. [DOI:10.1111/cpr.12143]
14. Soule, H., Vasquez, J., Long, A., Albert, S. and Brennan, M. 1973. A human cell line from a pleural effusion derived from a breast carcinoma. - J. Natl. Cancer Inst. 51: 1409-1416. [DOI:10.1093/jnci/51.5.1409]
15. Srivastava, V., Negi, A. S., Kumar, J. K., Gupta, M. M. and Khanuja, S. P. 2005. Plant-based anticancer molecules: a chemical and biological profile of some important leads. - Bioorg Med. Chem. 13: 5892-5908. [DOI:10.1016/j.bmc.2005.05.066]
16. Tariq, A., Sadia, S., Pan, K., Ullah, I., Mussarat, S., Sun, F., Abiodun, O. O., Batbaatar, A., Li, Z., Song, D., Xiong, Q., Ullah, R., Khan, S., Basnet, B. B., Kumar, B., Islam, R. and Adnan, M. 2017. A systematic review on ethnomedicines of anti-cancer plants. - Phytother Res. 31: 202-264. [DOI:10.1002/ptr.5751]
17. WHO. 2014. Global status report on noncommunicable diseases. In: MENDIS, H. (Ed.). Geneva: World Health Organization.
18. Wolbis, M. 1989. Flavonol glycosides from sedum album. - Phytochem. 28: 2187-2189. [DOI:10.1016/S0031-9422(00)97941-X]
19. Zhou, Y., Zheng, J., Li, Y., Xu, D. P., Li, S., Chen, Y. M. and Li, H.B. 2016. Natural polyphenols for prevention and treatment of cancer. - Nutrients 8: 515. [DOI:10.3390/nu8080515]

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