The effect of plasma water on the anatomical structure of beans (Vicia faba)

Authors

1 Assistant Professor, Department of Agronomy, Ramh.C., Islamic Azad University, Ramhormoz, Iran

2 Corresponding author,Associate Professor,Department of Biology, Ar.C., Islamic Azad University, Arak, Iran

Abstract
Objective: “Broad bean (Vicia faba) is important in many countries as a source of high protein percentage, amino acid ratios, mineral salts, and calcium. The use of new scientific methods and modified traditional methods such as plasma water have helped a lot to achieve of improved plant growth. This study was conducted to investigate the effect of plasma-activated water on growth and anatomical features of Vicia faba.





























Method: After preparation , seeds were first soaked for 24 hours with water that had been activated with plasma for 5 minutes and 20 minutes, and with control water without plasma application. After planting, the pots were watered twice a week for 40 to 50 days. After this period, the anatomical features were examined.



Results: The results showed that in plants treated with vascular bundle dispersion, the growth and development of xylem vessels and the growth of the vegetative apical meristem were greater than in the control And the treated plants entered the reproductive phase faster.

Conclusions: plasma-activated water can increase the growth of faba bean plants by causing changes in anatomical and developmental characteristics.





























According to the article results, use of plasma water is recommended to improve the performance of this plant.





Keywords


Adhikari, B., Adhikari, M., & Park, G. (2020). The effects of plasma on plant growth, development, and sustainability. Applied Sciences, 10(17), 6045
Filatova, I., Lyushkevich, V., Haimi, P., & Baniulis, D. (2019). Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed Phytohormone Balance, Seedling Development and Leaf Protein Expression. Scientific Reports, 9, 6437.
Gansert, D. (2003). Xylem sap flow as a major pathway for oxygen supply to the sapwood of birch (Betula pubescens Ehr.). Plant, Cell & Environment, 26(11), 1803-1814..
Guo, W., Xing, Y., Luo, X., Li, F., Ren, M., & Liang, Y. (2023). Reactive oxygen species: a crosslink between plant and human eukaryotic cell systems. International Journal of Molecular Sciences, 24(17), 13052.
Hayashi, N., Ono, R., Nakano, R., Shiratani, M., Tashiro, K., Kuhara, S., & Hagiwara, H. (2016). DNA microarray analysis of plant seeds irradiated by active oxygen species in oxygen plasma. Plasma Medicine, 6(3-4).
Ling, L., Jiafeng, J., Jiangang, L., Minchong, S., Xin, H., Hanliang, S., & Yuanhua, D. (2014). Effects of cold plasma treatment on seed germination and seedling growth of soybean. Scientific reports, 4(1), 5859.
Lee, K. H., Kim, H. J., Woo, K. S., Jo, C., Kim, J. K., Kim, S. H., & Kim, W. H. (2016). Evaluation of cold plasma treatments for improved microbial and physicochemical qualities of brown rice. Lwt, 73, 442-447.
Mujahid, Z., Tounekti, T., & Khemira, H. (2020). Cold plasma treatment to release dormancy and improve growth in grape buds: a promising alternative to natural chilling and rest breaking chemicals. Scientific Reports, 10(1), 2667.
Rahman, M. M., Sajib, S. A., Rahi, M. S., Tahura, S., Roy, N. C., Parvez, S., ... & Kabir, A. H. (2018). Mechanisms and signaling associated with LPDBD plasma mediated growth improvement in wheat. Scientific reports, 8(1), 10498.
Renáta, Š., Nicolette, V., Monika, B., Stanislav, K., Eliška, G., Veronika, M., & Ľudmila, S. (2021). Enhanced in situ activity of peroxidases and lignification of root tissues after exposure to non-thermal plasma increases the resistance of pea seedlings. Plasma Chemistry and Plasma Processing, 41, 903-922.
Ros Barceló, A. (2005). Xylem parenchyma cells deliver the H2O2 necessary for lignification in differentiating xylem vessels. Planta, 220(5), 747-756.
Safari, N., Iranbakhsh, A., & Ardebili, Z. O. (2017). Non-thermal plasma modified growth and differentiation process of Capsicum annuum PP805 Godiva in in vitro conditions. Plasma Science and Technology, 19(5), 055501 (In Persian).
Schippers, J. H., Foyer, C. H., & van Dongen, J. T. (2016). Redox regulation in shoot growth, SAM maintenance and flowering. Current opinion in plant biology, 29, 121-128.
Seddighinia, F. S., Iranbakhsh, A., Oraghi Ardebili, Z., Nejad Satari, T., & Soleimanpour, S. (2020). Seed priming with cold plasma and multi-walled carbon nanotubes modified growth, tissue differentiation, anatomy, and yield in bitter melon (Momordica charantia). Journal of plant growth regulation, 39, 87-98.
Sera, B., Stranák, V., Serý, M., Tichý, M., & Spatenka, P. (2008). Germination of Chenopodium album in response to microwave plasma treatment. Plasma Science and Technology, 10(4), 506.
Sivachandiran, L., & Khacef, A. (2017). Enhanced seed germination and plant growth by atmospheric pressure cold air plasma: combined effect of seed and water treatment. RSC advances, 7(4), 1822-1832.
Sonmez, M. C., Ozgur, R., & Uzilday, B. (2023). Reactive oxygen species: Connecting eustress, hormesis, and allostasis in plants. Plant Stress, 8, 100164.
Tong, J., He, R., Zhang, X., Zhan, R., Chen, W., & Yang, S. (2014). Effects of atmospheric pressure air plasma pretreatment on the seed germination and early growth of Andrographis paniculata. Plasma Science and Technology, 16(3), 260.
Vavilala, S. L., Gawde, K. K., Sinha, M., & D’Souza, J. S. (2015). Programmed cell death is induced by hydrogen peroxide but not by excessive ionic stress of sodium chloride in the unicellular green alga Chlamydomonas reinhardtii. European Journal of Phycology, 50(4), 422-438.
Wang, X. Q., Zhou, R. W., Groot, G. D., Bazaka, K., Murphy, A. B., & Ostrikov, K. (2017). Spectral characteristics of cotton seeds treated by a dielectric barrier discharge plasma. Scientific reports, 7(1), 5601.
Yodpitak, S., Mahatheeranont, S., Boonyawan, D., Sookwong, P., Roytrakul, S., & Norkaew, O. (2019). Cold plasma treatment to improve germination and enhance the bioactive phytochemical content of germinated brown rice. Food chemistry, 289, 328-339.
Zhang, S., Rousseau, A., & Dufour, T. (2017). Promoting lentil germination and stem growth by plasma activated tap water, demineralized water and liquid fertilizer. RSC advances, 7(50), 31244-31251.
Zhang, J. J., Jo, J. O., Huynh, D. L., Mongre, R. K., Ghosh, M., Singh, A. K., & Jeong, D. K. (2017). Growth-inducing effects of argon plasma on soybean sprouts via the regulation of demethylation levels of energy metabolism-related genes. Scientific Reports, 7(1), 41917.

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026