Nova Biologica Reperta

Impact of Fraxinus Excelsior Leaf Extract on Post-Thaw Quality Parameters of Mouse (Mus Musculus) Sperm

Authors

Mahdi Jafarbeglou
Mahnaz Azarnia
Seyede Ghadireh Mirabolghasemi

Kharazmi

https://doi.org/https://doi.org/10.22034/NBR.12.4.5
Abstract
Background: Cryopreservation of sperm is a critical technique in reproductive biology and medicine, allowing for long-term storage and use of genetic material. However, the freezing and thawing process can significantly impact sperm quality, affecting parameters such as motility, viability, and overall fertility potential.

Aim: This study explores the effect of an aqueous-alcoholic extract of Fraxinus excelsior on the quality parameters of sperm in NMRI mice after cryopreservation.

Methods: This study uses a descriptive correlation approach to examine the relationship between variables in sperm motility and cytoplasmic membrane health. Thirty male NMRI mice were divided into five experimental groups: healthy control, solvent control, and Fraxinus excelsior extract. Sperm motility, cytoplasmic membrane health, and oxidative stress levels were measured. Data analysis was performed using SAS 9.4 software, with mean comparisons using Duncan's test at a significance level of 0.05.

Keywords

Fraxinus excelsior (ash) plant leaves
freezing
thawing
sperm
aqueous-alcoholic extract

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026

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