Green synthesis of silver nanoparticles using Verbascum thapsus extract and its effect on BfmR gene expression in clinical isolates of Acinetobacter baumannii

Sefati, Sadaf; Behboodian, Bita; Dolatabadi, Samaneh

doi:10.22034/11.4.67

Green synthesis of silver nanoparticles using Verbascum thapsus extract and its effect on BfmR gene expression in clinical isolates of Acinetobacter baumannii

Authors

Sadaf Sefati

Bita Behboodian

Samaneh Dolatabadi

10.22034/11.4.67

Abstract

Acinetobacter baumannii is an opportunistic multidrug-resistant pathogen that causes respiratory and urinary tract infections. Therefore, the development of an alternative drug against Acinetobacter is of great importance. The aim of this study was to investigate the antibacterial activity of silver nanoparticles synthesized by a green method on the growth of Acinetobacter baumannii and the expression of the BfmR gene. In this study, silver nanoparticles were synthesized in combination with Verbascum thapsus extract and their characteristics were determined by X-ray diffraction, spectrophotometry, TEM electron microscopy and zeta potential analysis. The antibacterial activity was investigatead by MIC analysis. Also, the effect of nanoparticles on the expression of the BfmR gene was investigated by real-time PCR. The prepared green nanoparticles had a size of about 30 nm. The MIC analysis showed that the nanoparticles have antibacterial activity against Acinetobacter baumannii. Gene expression results also indicate a mechanism of down-regulation of BfmR .also nanoparticles leads to reduced biofilm formation. silver nanoparticles, have gained widespread popularity as antibacterial options due to their unique physical and chemical properties and broad-spectrum antibacterial activity. Nanoparticles obtained from flower extract can reduce BfmR gene expression, thereby inhibiting biofilm formation and exerting antibacterial effects.

Keywords

Acinetobacter baumannii

Verbascum thapsus

nanoparticle

biofilm

BfmR gene