Investigating the effects of combined application of iron oxide nanoparticles (Fe2O3) and alfalfa on growth parameters and pathology of digestive tube, liver and kidney of Koi fish(Cyprinus Carpio)

Pourmohammad, Mohammadreza; Khayatzadeh, Jina; Hanafi, Vahid; Iranshahi, Mehrdad

doi:10.22034/NBR.11.3.61

Investigating the effects of combined application of iron oxide nanoparticles (Fe2O3) and alfalfa on growth parameters and pathology of digestive tube, liver and kidney of Koi fish(Cyprinus Carpio)

Authors

Mohammadreza Pourmohammad ¹

Jina Khayatzadeh ²

Vahid Hanafi ²

Mehrdad Iranshahi ³

¹ Ilam University of Medical Sciences

² Islamic Azad University, Mashhad Branch

³ Mashhad University of Medical Sciences

https://doi.org/10.22034/NBR.11.3.61

Abstract

Background: Nowadays, due to the increasing growth of production and consumption of nanoparticles, problems such as their entry into fersh waters and aquatic poisoning have arisen. Iron oxide nanoparticles are among the most widely used in this field. Alfalfa (Medicago sativa), a flowering plant belonging to the legume family, contains high amounts of protein, calcium, various vitamins and antioxidant effects. The aim of this research was to investigate the combined effect of iron oxide nanoparticles (Fe2O3)and alfalfa plant on growth parameters and pathology of digestive tract, liver and kidney of koi fish (an example of carp fish with a variety of colors and eye-catching effect).

Materials and Methods: In this experimental research, the number of 60 koi fish larvae were randomly divided into 6 groups including the control group and 5 experimental groups. The effect of feeding koi fish larvae with biomar combined with 10% and 20% alfalfa and being exposed to iron oxide nanoparticles with a concentration of 100 mg/l for 14 days in 5 treatment groups was investigated in comparison with the control group. The collected data were analyzed by Excel and SPSS 20 statistical software.

Keywords

koi fish

alfalfa

Histology

iron oxide nanoparticles

20.1001.1.24236330.1403.11.3.5.9

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