Using machine learning to model different levels of salinity stress and silica fertilization of fenugreek (Trigonella foenum-graecum L.)

Authors

Behbahan Khatam Alanbia University of Technology

Abstract
In recent years, the use of machine learning methods in various fields of agriculture is increasing, and these methods provide us with very good information for predicting and checking different levels of performance in plants. In the current research, according to the results of the preliminary experiment carried out previously with specific levels of salinity stress and fertilization (salinity stress levels of zero, 75 and 150 mM sodium chloride and fertilization levels of zero and 3 grams per liter of silica) which were previously carried out and using the nonlinear regression model (NLR) and Python programming language, the morphological and physiological traits of the fenugreek medicinal plant at the newly defined levels of salinity stress and silica fertilization (salinity of up to 300 mM level and silica fertilization in two levels of 1 and 2 grams per liter) were predicted without conducting practical tests and based on the levels of salinity and initial fertilization. The non-linear regression model is a widely used algorithm in data analysis where the relationship between variables is non-linear and can create meaningful relationships between variables using non-linear functions. The results showed that the positive effect of silica on the amount of chlorophyll fluorescence (Fv/Fm) can be seen from zero to 180 mM salinity level and the amount of greenness index (SPAD) from zero to 100 mM salinity level. It seems that according to the results of the present research, it is possible to use machine learning to investigate and analyze the morphological and physiological characteristics of the fenugreek medicinal plant at other defined levels of salinity stress and other defined silica fertilization with no need conduct a practical experiment.

Keywords


Al-aghabary, K., Zhu, Z. & Shi, Q. 2005. Influence of silicon supply on chlorophyll content, chlorophyl fluorescence, and antioxidative enzym activities in tomato plants under salt stress. Journal of Plant Nutrition, 27: 2101-2115. DOI: 10.1081/PLN-200034641
Agrawal, S.C. 2021. Deep learning based non-linear regression for Stock Prediction, IOP Conference Series: Materials Science and Engineering, 1116: 012189. DOI: 10.1088/1757-899X/1116/1/012189
Amuthaselvi, G. & Ambrose, D.C.P. 2016. Fenugreek. CABI Press. India.
Arouiee, H., Nasseri, M., Neamati, H., & Kafi, M. 2014. Effects of Silicon on Salinity tolerance in fenugreek (Trigonella foenum- graecum L.). Applied Field Crops Research, 27(104), 165-172. DOI: 10.22092/aj.2014.101835 (In Persian)
Banakar, M.H., Ranjbar, G.H., & Soltani, V. 2012. Physiological response of some forage halophytes under saline conditions. Environmental Stresses in Crop Sciences, 5(1), 55-65. DOI: 10.22077/escs.2012.114 (In Persian)
Chowdhury, M.M.U., Bhowal, S.K., Farhad, I.S.M., Choudhury, A.K. & Khan, A.S. 2014 Productivity of fenugreek varieties Trigonella foenum-graecum L. in the coastal saline areas of noakhali. The Agriculturists, 12: 18-23. DOI: 10.3329/agric.v12i2.21726
Chen, W., Yao, X., Cai, K. & Chen, J. 2011. Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption. Biological Trace Element Research, 142, 67-76. DOI: 10.1007/s12011-010-8742-x
Deshmukh, R.K., Vivancos, J., Guerin, V., Sonah, H., Labbe, C., Belzile, F. & Belanger, R. R. 2013. Identification and functional characterization of silicon transporters in soybean using comparative genomics of major intrinsic proteins in Arabidopsis and rice. Plant Molecular Biology, 83, 303-315. DOI: 10.1007/s11103-013-0087-3
Debona, D., Rodrigues, F.A. & Datnoff, L.E. 2017. Silicon's role in abiotic and biotic plant stresses. Annual Review of Phytopathology, 55, 85-107. DOI: 10.1146/annurev-phyto-080516-035312
Fani E. 2022. Physiological and biochemical responses of basil (Ocimum basilicum) to silicon spraying under salinity stress. Journal of Crop Production, 15(3), 123-136. DOI: 10.22069/ejcp.2022.19571.2459 (In Persian)
Fani E. 2023. The effects of feeding by silica fertilizer on the reduction of stress caused by salinity in fenugreek plants, Journal of Soil Management and Sustainable Production, in press. (In Persian)
Fani, E. & Hajihashemi, S. 2023. Investigation of the effect of silica spraying and salinity stress on some physiological traits of Camelina sativa oil plant. Journal of Plant Environmental Physiology, 69 (1): 149- 159. DOI: 10.30495/iper.2022.1954207.1780. (In Persian)
Gupta, D.K., Prasad, R., Kumar, P., Mishra, V.N., Dikshit, P.K.S., Dwivedi, S.B. & Srivastava, P.K. 2015. Crop variables estimation by adaptive neuro-fuzzy inference system using bistatic scatterometer data. In Microwave and Photonics ICMAP. International Conference on Microwave and Photonics ICMAP, pp. 1-2, DOI: 10.1109/ICMAP.2015.7408756.
Hajihashemi S., Jahantigh O. & Fani E. 2023. The effect of silicon treatment on improving the physiological response of radish (Raphanus sativus L.) to salinity stress. Plant Process and Function 11 (47): 2. DOR: 20.1001.1.23222727.1401.11.47.3.2 (In Persian)
Hasanuzzaman, M., Nahar, K. & Fujita, M. 2013. Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In: Ecophysiology and Responses of Plants under Salt Stress. eds. Ahmad, P., Azooz, M. M. and Prasad, M. N. V. Springer. New York.
Haghighi, M. & Masoumi, Z. 2021. Effect of caffeic acid on growth and reducing the destructive effects of salinity on greenhouse cucumber Cucumis sativus var. Super daminos. Journal of Vegetables Sciences, 48, 35-51. DOI: 10.22034/iuvs.2021.131965.1114
Hosseini, M., Agereh, S.R, Khaledian, Y., Zoghalchali, H.J., Brevik, E.C. & Naeini, S.A. 2017. Comparison of multiple statistical techniques to predict soil phosphorus. Applied Soil Ecology, 114, 123-131. DOI: 10.1016/j.apsoil.2017.02.011
Hurtado, A.C, Chiconato, D.A., Prado, R. de M., Sousa Junior, G. da S., Gratao, P.L., Felisberto, G. & Mathias dos Santos, D.M. 2020. Different methods of silicon application attenuate salt stress in sorghum and sunflower by modifying the antioxidative defense mechanism. Ecotoxicology and Environmental Safety, 203, 110964-110975. DOI: 10.1016/j.ecoenv.2020.110964
Kao, W.Y., Tsai, T.T., Tsai, H.C. & Shi, C.N. 2006. Response of three Glycine species to salt stress. Environmental and Experimental Botany, 56, 120-125. DOI: 10.1016/j.envexpbot.2005.01.009
Lee, M. H., Cho, E.J., Wi, S.G., Bae, H., Kim, J.E., Cho, J.Y., Lee, S., Kim, J.H. & Chung, B.Y. 2013. Divergences in morphological changes and antioxidant responses in salt-tolerant and salt-sensitive rice seedlings after salt stress. Plant Physiology and Biochemistry, 70, 325-335. DOI: 10.1016/j.plaphy.2013.05.047
Liang, Y., Sun, W., Zhu, Y.G., & Christie, P. 2007. Mechanisms of silicon mediated alleviation of a biotic stresses in higher plants: a review. Environmental Pollution, 147, 422-428. DOI: 10.1016/j.envpol.2006.06.008
Liu, B., Soundararajan, P. & Manivannan, A. 2019. Mechanisms of silicon-mediated amelioration of salt stress in plants. Plants, 8, 307. DOI: 10.3390/plants8090307
Luyckx, M., Hausman, J.F., Lutts, S. & Guerriero, G. 2017a. Impact of silicon in plant biomass production: Focus on bast fibres, hypotheses, and perspectives. Plants Basel, 6, 37. DOI: 10.3390%2Fplants6030037
Luyckx, M., Hausman, J.F., Lutts, S. & Guerriero, G. 2017b. Silicon and Plants: Current Knowledge and Technological Perspectives. Frontiers in Plant Science, 8, 411. DOI: 10.3389/fpls.2017.00411
Ma, J.F. & Takahashi, E. 2002. Soil, Fertilizer, and Plant Silicon Research in. Elsevier Science, Amsterdam.
Payamani, R., Nosratti, I. & Amerian, M. 2021. Effect of different levels of salinity, nitrogen and Torilis arvensis competition on growth characteristics and leaf yield of Coriander Coriandrum sativum. Journal of Vegetables Sciences, 51, 51- 62. DOI: 10.22034/iuvs.2021.526940.1153
Rico-Chávez, A.K., Franco, J.A., Fernandez-Jaramillo, A.A., Contreras-Medina, L.M., Ramón Gerardo Guevara-González, R.G. & Hernandez-Escobedo, Q. 2022. Machine Learning for Plant Stress Modeling: A Perspective towards Hormesis Management. Plants, 117, 970. DOI: 10.3390%2Fplants11070970
Szalay, L., Hegedus, A. & Stefanovitis-Banyai, E. 2005. Presumable protective role of peroxidase and polyphenol oxidase enzymes against freezing stress in peach Prunus persica L. Batsch. Acta Biologica Szegediensis, 491- 2, 121-122.
Tuna, A.L., Kaya, C., Higgs, D.E.B., Murillo- Amador, B., Aydemir, S. & Girgin, A.R. 2008. Silicon improves salinity tolerance in wheat plants. Environmental and Experimental Botany, 621, 10-16. DOI: 10.1016/j.envexpbot.2007.06.006
Wang, X.S. & Han, J.G. 2007. Effects of NaCl and silicon on ion distribution in the roots, shoots and leaves of two alfalfa cultivars with different salt tolerance. Soil Science and Plant Nutrition, 53, 278-285. DOI: 10.1111/j.1747-0765.2007.00135.x
Zahir, M. & Hussain, F. 2010. Vegetative growth performance of five medicinal plants under NaCl salt stress. Pakistan Journal of Botany, 42, 303-316.
Zargari A. 1371. Medicinal Plants. Volume 1, Tehran University Press, Tehran (In Persian)
Zuccarini, P. 2008. Effects of silicon on photosynthesis, water relations and nutrient uptake of Phaseolus vulgaris under NaCl stress. Biologia Plantarum, 52, 157-160. DOI: 10.1007/s10535-008-0034-3

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026