Evaluation of freezing tolerance in chickpea promising genotypes (Cicer arietinum) using physiological traits and molecular markers

Shokoohi, Elmira; Sofalian, Omid; Asghari, Ali; Khomari, Saeid; Esmaielpour, Behrooz; Aflatooni, Hamed

Evaluation of freezing tolerance in chickpea promising genotypes (Cicer arietinum) using physiological traits and molecular markers

Authors

Elmira Shokoohi

Omid Sofalian

Ali Asghari

Saeid Khomari

Behrooz Esmaielpour

Hamed Aflatooni

Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Chickpea is one of the most important plants of the legume family and is very important in the diet. In order to investigate the genetic diversity of chickpea, an experiment was conducted with 18 chickpea genotypes in the form of a completely to investigate the genetic diversity of chickpea, an experiment was conducted with 18 chickpea genotypes in the form of a completely randomized block design. After acclimatization of plants to cold, freezing treatment was applied at temperatures of -6, -8 and -10 and their 50% lethality temperature (LT₅₀) was determined by probit transformation. Before and after the habituation stage, a leaf sample was taken and the relative content of leaf water, photosynthetic pigments, proline, soluble sugar, protein percentage, catalase, peroxidase, polyphenol oxidase and greenness index were measured. Genotype number 5 with the lowest LT₅₀ (-8.86) and the highest survival percentage (80%) was the most resistant genotype and genotype 10 with the highest LT₅₀ (-3.57) and the lowest survival percentage along with genotype 15 were recognized as the most sensitive genotypes. In order to evaluate genetic diversity, DNA extraction was utilized and 21 different ISSR primers were used in the investigation. The results showed the presence of polymorphism among the cultivars studied. A total of 101 clear bands were produced, of which 94 were polymorphic bands. Polymorphic information content (PIC) was in the range of 0.332 (initiator 7) to 0.049 (initiator 16). The amount of gene diversity was between 0.126 and 0.977 changes. Cluster analysis of genotypes was done using Jaccard similarity coefficient and UPGMA method

Keywords

genetic variation

ISSR

LT50

physiological characteristics

vernalization

20.1001.1.24236330.1402.10.2.5.0

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