یافته های نوین در علوم زیستی

سنتز برون سلولی سبز نانوذرات Fe2O3 توسط سویه باکتری بومی آبزی آلکالیژنز NV06

نویسندگان

مراحم آشنگرف
نسترن وکیلی سهرفروزانی

گروه علوم زیستی، دانشکده علوم پایه، دانشگاه کردستان، سنندج، ایران

https://doi.org/10.52547/nbr.9.3.182
چکیده
در پژوهش حاضر از باکترهای آبزی به ‌عنوان زیست کاتالیزگر برای تبدیل زیستی FeCl3 به نانو ذرات Fe2O3 استفاده شد. در مجموع 25 سویه باکتری آبزی در محیط کشت تریپتیک ‌سوی ‌آگار غنی شده با 10میلی مولار FeCl3 بر اساس تکنیک غنی‌سازی جداسازی شد. در بین سویه­های باکتری‌های مورد آزمایش، تنها سویه NV06 قادر به سنتز برون سلولی نانوذرات Fe2O3 بود که در نهایت بر اساس ویژگی‌های فنوتیپی و مولکولی به‌ عنوان Alcaligenes sp. strain NV06 مورد شناسایی قرار گرفت. سنتز برون سلولی نانوذرات آهن تولید شده توسط این سویه، تحت شرایط بهینه‌ واکنش مورد بررسی قرار گرفت. نانوذرات سنتز شده توسط سویه باکتریایی NV06، به­وسیله‌ تجزیه و تحلیل­های طیف سنجی UV-Vis، تجزیه و تحلیل­های میکروسکوپ الکترونی روبشی، آزمون طیف سنجی پراش انرژی پرتو ایکس و طیف سنجی تبدیل فوریه مادون قرمز تعیین خصوصیت شد. نتایج به‌دست آمده نشان داد که نانوذرات میله­ای اکسید آهن توسط عصاره عاری از سلول باکتری مذکور، در دمای بهینه‌ 28 درجه سانتیگراد، pH بهینه‌ برابر 6 و در غلظت 10 میلی‌مولار FeCl3، به مدت 96 ساعت گرماگذاری در دور شیکر rpm150، با میانگین اندازه‌ ابعاد طولی 2/80 نانومتر و میانگین قطری 5/25 نانومتر تولید می‌شوند. در این پژوهش، برای نخستین بار سنتز برون سلولی نانوذرات اکسیدآهن فریک با اندازه مطلوب از طریق استراتژی عصاره عاری از سلول در سرده Alcaligenes گزارش شد. امید است نتایج این پژوهش بتواند ظرفیت‌های بالقوه‌ میکروب­های آبزی به­عنوان زیست کاتالیزگرهای ایمن، ساده و مؤثر در تولید نانوذرات Fe2O3 را معرفی نماید.



کلیدواژه‌ها

باکتری آبزی
زیست کاتالیزگر
طیف سنجی
عصاره عاری از سلول
نانو اکسیدآهن فریک

عنوان مقاله English

Green extracellular synthesis of the Fe2O3 nanoparticles by a native marine bacterium, Alcaligenes sp. strain NV06

نویسندگان English

Morahem Ashengroph
Nastaran Vakili Sohrforouzani
Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran
چکیده English

This study investigated the potential of aquatic bacteria for their ability as a biocatalyst to synthesized Fe2O3 nanoparticles using iron precursor, FeCl3. A total of 25 aquatic bacterial strains were isolated in trypticase soy agar plus 10 mM FeCl3 with selective enrichment technique. Among the bacterial strains evaluated, NV06 was the only strain able to synthesize Fe2O3 nanoparticles extracellularly. The strain NV06 was identified as Alcaligenes sp., on the basis of phenotypic and molecular characteristics. Extracellular synthesis of Fe2O3 nanoparticles by this strain was investigated under the optimal conditions. The biosynthesized Fe2O3 nanoparticles were characterized using UV–visible spectrophotometry (UV-Vis), Scanning electron microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX), and Fourier transform infrared (FTIR) spectroscopy. The results showed that cell-free extract (CFE) of the bacterium strain can produce the rod-shaped Fe2O3 nanoparticles with mean edge lengths of 80.2 nm and mean diameters of 25.5 nm, after being exposed to FeCl3 solution (10 mM), at an optimum pH of 6 and an optimum temperature of 28 °C, after 96 hours of incubation at 150 rpm. This is the first report on the extracellular biosynthesis of Fe2O3 nanoparticles using the genus of Alcaligenes under the CFE strategy. It could be speculated that the results of the study can hopefully introduce the inherent capabilities of aquatic microbes as safe, simple, and effective biocatalysts in the production of Fe2O3 nanoparticles.

کلیدواژه‌ها English

aquatic bacterium
biocatalyst
cell-free extract
ferric oxide nanoparticle
spectroscopy
Abdeen, M., Sabry, S., Ghozlan, H., El-Gendy, A.A. & Carpenter, E.E. 2016. Microbial-physical synthesis of Fe and Fe3O4 magnetic nanoparticles using Aspergillus niger YESM1 and supercritical condition of ethanol. Journal of Nanomaterials 2016: 1-7.
Abo‐zeid, Y. & Williams, G.R. 2019. The potential anti‐infective applications of metal oxide nanoparticles: A systematic review. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology 12: 1-36.
Arias, L., Pessan, J., Vieira, A., Lima, T., Delbem, A. & Monteiro, D. 2018. Iron oxide nanoparticles for biomedical applications: A perspective on synthesis, drugs, antimicrobial activity, and toxicity. Antibiotics 7: 1-32.
Ashengroph, M. & Sahami-Soltani, M. 2018. Antimicrobial effects of extracellular copper sulfide nanoparticles synthesized from Bacillus licheniformis. Journal of Microbial World 11: 243-257.
Ashengroph, M., Khaledi, A. & Bolbanabad, E.M. 2020. Extracellular biosynthesis of cadmium sulphide quantum dot using cell-free extract of Pseudomonas chlororaphis CHR05 and its antibacterial activity. Process Biochemistry 89: 63-70.
Ashengroph, M. & Hosseini, S.R. 2021. A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacterial properties. International Microbiology 24: 103-114.
Baker, S., Harini, B.P., Rakshith, D. & Satish, S. 2013. Marine microbes: invisible nanofactories. Journal of Pharmacy Research 6: 383-388.
Balamurughan, M.G., Mohanraj, S., Kodhaiyolii, S. & Pugalenthi, V. 2014. Ocimum sanctum leaf extract mediated green synthesis of iron oxide nanoparticles: spectroscopic and microscopic studies. Journal of Chemical and Pharmaceutical Sciences 4: 201-204.
Bereswill, S., Bugert, P., Bruchmüller, I. & Geider, K. 1995. Identification of the fire blight pathogen, Erwinia amylovora, by PCR assays with chromosomal DNA. Applied and Environmental Microbiology 61: 2636-2642.
Bharde, A.A., Parikh, R.Y., Baidakova, M., Jouen, S., Hannoyer, B., Enoki, T., Prasad, B.L., Shouche, Y.S., Ogale, S. & Sastry, M. 2008. Bacteria-mediated precursor-dependent biosynthesis of superparamagnetic iron oxide and iron sulfide nanoparticles. Langmuir 24: 5787-5794.
Bhargava, A., Jain, N., Barathi, M., Akhtar, M.S., Yun, Y.S. & Panwar, J. 2013. Synthesis, characterization and mechanistic insights of mycogenic iron oxide nanoparticles. Nanotechnology for Sustainable Development 15: 337-348.
Bolbanabad, E.M., Ashengroph, M. & Darvishi, F. 2020. Development and evaluation of different strategies for the clean synthesis of silver nanoparticles using Yarrowia lipolytica and their antibacterial activity. Process Biochemistry 94: 319-328.
Chauhan, S. & Upadhyay, L.S.B. 2019. Biosynthesis of iron oxide nanoparticles using plant derivatives of Lawsonia inermis (Henna) and its surface modification for biomedical application. Nanotechnology for Environmental Engineering 4: 1-10.
Crespo, K.A., Baronetti, J.L., Quinteros, M.A., Páez, P.L. & Paraje, M.G. 2017. Intra- and extracellular biosynthesis and characterization of iron nanoparticles from prokaryotic microorganisms with anticoagulant activity. Pharmaceutical Research 34: 591-598.
Dinali, R., Ebrahiminezhad, A., Manley-Harris, M., Ghasemi, Y. & Berenjian, A. 2017. Iron oxide nanoparticles in modern microbiology and biotechnology. Critical Reviews in Microbiology 43: 493-507.
Ealias, A.M. & Saravanakumar, M.P. 2017. A review on the classification, characterisation, synthesis of nanoparticles and their application. IOP Conference Series: Materials Science and Engineering 263: 032019.
Hoag, G.E., Collins, J.B., Holcomb, J.L., Hoag, J.R., Nadagouda, M.N. & Varma, R.S. 2009. Degradation of bromothymol blue by ‘greener’nano-scale zero-valent iron synthesized using tea polyphenols. Journal of Materials Chemistry 19: 8671-8677.
Jagathesan, G. & Rajiv. P. 2018. Biosynthesis and characterization of iron oxide nanoparticles using Eichhornia crassipes leaf extract and assessing their antibacterial activity. Biocatalysis and Agricultural Biotechnology 13: 90-94.
Jeevanandam, J.. Chan, Y.S. & Danquah, M.K. 2016. Biosynthesis of metal and metal oxide nanoparticles. ChemBioEng Reviews 3: 55-67.
Kumar, S., Stecher, G. & Tamura, K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33: 1870-1874.
Manivasagan, P., Nam, S.Y. & Oh, J. 2016. Marine microorganisms as potential biofactories for synthesis of metallic nanoparticles. Critical Reviews in Microbiology 42: 1007-1019.
Mohamed, Y.M., Azzam, A.M., Amin, B.H. & Safwat, N.A. 2015. Mycosynthesis of iron nanoparticles by Alternaria alternata and its antibacterial activity. African Journal of Biotechnology 14: 1234-1241.
Murray, M.G. & Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321-4326.
Pat‐Espadas, A.M. & Cervantes, F.J. 2018. Microbial recovery of metallic nanoparticles from industrial wastes and their environmental applications. Journal of Chemical Technology and Biotechnology 93: 3091-3112.
Rajeshwari, S., Pattanathu, K.S.M., Rahman, Rajiv, P., Narendhran, S. & Venckatesh, R. 2014. Biosynthesis and characterization of Acalypha indica mediated copper oxide nanoparticles and evaluation of its antimicrobial and anticancer activity. Spectrochimica Acta, Part A: Molecular and Bimolecular Spectroscopy 129: 255-258.
Revati, K. & Pandey, B.D. 2011. Microbial synthesis of iron-based nanomaterials-A review. Bulletin of Materials Science 34: 191-198.
Sharaf, S.M., Abbas, H.S. & Ismaeil, T.A. 2019. Characterization of spirugenic iron oxide nanoparticles and their antibacterial activity against multidrug-resistant Helicobacter pylori. Egyptian Journal of Phycology 20: 1-28.
Singh, J., Dutta, T., Kim, K.H., Rawat, M., Samddar, P. & Kumar, P. 2018. Green synthesis of metals and their oxide nanoparticles: applications for environmental remediation. Journal of Nanobiotechnology 16: 1-24.
Soosani, N., Ashengroph, M. & Chehri, Kh. 2021. Extracellular green synthesis of zinc oxide nanoparticle by using the cell-free extract Rhodotorula pacifica NS02 and investigation of their antimicrobial activities. Nova Biologica Reperta 8: 195-205. (In Persian)
Sundaram, P.A., Augustine, R. & Kannan, M. 2012. Extracellular biosynthesis of iron oxide nanoparticles by Bacillus subtilis strains isolated from rhizosphere soil. Biotechnology and Bioprocess Engineering 17: 835-840.
Van Trappen, S., Tan, T.L., Samyn, E. & Vandamme, P. 2005. Alcaligenes aquatilis sp. nov., a novel bacterium from sediments of the Weser Estuary, Germany, and a salt marsh on Shem Creek in Charleston Harbor, USA. International Journal of Systematic and Evolutionary Microbiology 55: 2571-2575.
Weisburg, W.G., Barns, S.M., Pelletier, D.A. & Lane, D.J. 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173: 697-703.

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

صفحه اصلی

راهنمای نویسندگان

ارسال مقاله

داوران

تماس با ما