Effect of foliar application of seaweed Ascophyllum nodosum extract on morpho-physiological characteristics of bean (Phaseolus vulgaris) under water stress

Alizadeh, Mohsen; Armand, Nezam; Rahimi, Maryam; Haji Hashemi, Shokoufeh

doi:10.52547/nbr.9.1.61

Effect of foliar application of seaweed Ascophyllum nodosum extract on morpho-physiological characteristics of bean (Phaseolus vulgaris) under water stress

Authors

Mohsen Alizadeh ¹

Nezam Armand ²

Maryam Rahimi ¹

Shokoufeh Haji Hashemi ²

¹ Department of Biology, Faculty of Sciences, Malayer University, Malayer, Iran

² Department of Biology, Faculty of Sciences, Behbahan Khatam Alanbia University of Technology, Khuzestan, Iran

https://doi.org/10.52547/nbr.9.1.61

Abstract

Seaweed extract can improve the physicochemical properties of the soil and has a favorable effect on the plant growth and development due to having high nutrient content, high water holding capacity, plant growth regulators and beneficial microorganisms. The aim of this experiment was to investigate the effect of Ascophyllum nodosum extract, a brown alga, on the morphophysiological characteristics of bean plant (Phaseolus vulgaris) under water stress. The experiment was performed in a Completely Randomized Factorial Design with three replications. Experimental treatments included 4 concentrations of foliar application of seaweed extract (0, 0.2, 0.4 and 0.6%) and 3 levels of water stress including no stress, mild stress and severe stress (irrigation at 75, 50 and 25% of field capacity, respectively). The results showed that the interaction effects of stress and algal extract on the evaluated traits were not significant. However, the seaweed extract significantly increased the leaf area, number of the leaves, leaf length, surface and length of the roots, root dry weight, the content of chlorophyll a and b and the total chlorophyll content. Application of algal extract at levels of 0.2 and 0.4% caused a significant increase in the evaluated parameters as compared with the control plants. The evaluation of water scarcity stress effects showed a significant reduction in the all studied characteristics in accordance with increasing water stress level from 75% to 25% of field capacity. The results of this study showed that foliar application of seaweed extract significantly increased some morphological and photosynthetic properties, while water stress significantly reduced the investigated parameters.

Keywords

bean

growth traits

Photosynthetic pigments

Water stress

20.1001.1.24236330.2022.9.1.6.6

Abrishamchi, P., Ganjeali, A. & Sakeni, H. 2012. Evaluation of morphological traits, proline content and antioxidant enzymes activity in chickpea genotypes (Cicer arietinum L.) under drought stress. Iranian Journal of Pulses Research 3: 17-30. [In Persian].
Ahmadpour, R., Hosseinzadeh, S.R. & Chashiani, S. 2016. Study of root morpho-physiological and biochemical characteristics of lentil (Lens culinaris Medik.) in response to moisture stress. Journal of Iranian Plant Echophysiological Resarch 2: 123-135. [In Persian].
Ahmadpour, R., Hosseinzadeh, S.R., Armand, N. & Chashiani, S. 2017. Evaluation of growth features, photosynthetic pigments and antioxidant enzymes activity of lentils cultivars in response to water stress. Nova Biologica Reperta 4: 226-235.
Ahmadpour, R., Rostami, M. & Hosseinzadeh, S.R. 2019. Influence of compost tea on seedling growth and germination indices of bean (Phaseolus vulgaris L.) in order to moderated negative effects caused by drought stress. Journal of Plant Researches 32: 474-488. [In Persian].
Ahmadpour, R., Salimi, A., Zeidi, H., Armand, N. & Hosseinzadeh, S.R. 2019. Effect of seaweed extract (Ascophyllum nodosum) on the stimulation of germination indices of chickpea (Cicer arietinum L.) under drought stress. Nova Biologica Reperta 6: 206-216. [In Persian].
Amiri, H., Ismaili, A. & Hosseinzadeh, S.R. 2017. Influence of vermicompost fertilizer and water deficit stress on morpho-physiological features of chickpea (Cicer arietinum L. cv. Karaj). Compost Science and Utilization 26: 1-14.
Armand, N., Amiri, H. & Ismaili, A. 2015. Interaction of methanol spray and water deficit stress on photosynthesis and biochemical characteristics of Phaseolus vulgaris L. cv. Sadry. Photochemistry and Photobiology 92: 1-219.
Bayoumi, T.Y., Eid, M. & Metwali, E.M. 2008. Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. African Journal of Biotechnology 7: 2341-2352.
Caffagni, D.E., Camargo, E., Casali, C.A., Lombardi, A.T. & Lima, M.I.S. 2015. Coupling microalgal cultures with hydroponics: Prospection for clean biotechnology processes. Journal of Algal Biomass Utilization 6: 88-94.
Craigie, J.S. 2011. Seaweed extract stimuliation in plant science and agriculture. Journal of Apply Phycology 23:371-393.
Flexas, J. & Medrano, H. 2008. Drought-inhibition of photosynthesis in C3-plants: Stomatal and non-stomatal limitation revisited. Annual of Botany 18: 183-189.
Gamze, O., Mehmet Demir, K.A. & Mehmet, A.T. 2005. Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). Turkish Journal of Agriculture and Foresty 29: 237-242.
Ganjeali, A. & Bagheri, A. 2011. Evaluation of morphological characteristics of root chickpea (Cicer arietinum L.) in response to drought stress. Iranian Journal of Pulses Research 1: 101-110.
Ganjeali, A., Porsa, H. & Bagheri, A. 2011. Assessment of Iranian chickpea (Cicer arietinum L.) germplasms for drought tolerance. Agriculture Water Management 98: 1477-1484.
Ghannad, R., Akbari, F. & Madadkar Haghjou, M. 2017. Effect of blue-green and green algae Spirulina, Chlorella, Dunaliella and minerals on the stimulation of metabolic and biochemical processes of germination in Dracocephalum kotschyi Boiss. Seeds. Nova Biologica Reperta 3: 295-307.
Guerfel, M., Baccouri, O., Boujnah, D., Cha, W. & Zarrouk, M. 2008. Impacts of water stress on gas exchange, water elations, chlorophyll content & leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars. Scientia Horticulturae 1: 1-7.
Halmer, P. 2000. Commercial seed treatment technology. In: Black, M. & Bewley, J.D. (editors) Seed Technology and its Biological Basis. Sheffield Academic Press, Sheffield, 257-286.
Hosseinzadeh, S.R. & Ahmadpour, R. 2018. Evaluation of vermicompost fertilizer application on growth, nutrient uptake and photosynthetic pigments of lentil (Lens culinaris Medik.) under moisture deficiency conditions. Journal of Plant Nutrition 41: 1276-1284.
Hosseinzadeh, S.R., Amiri, H. & Ismaili, A. 2017. Nutrition and biochemical responses of chickpea (Cicer arietinum L.) to vermicompost fertilizer and water deficit stress. Journal of Plant Nutrition 40: 2259-2268.
Hosseinzadeh, S.R., Amiri, H. & Ismaili, A. 2016. Effect of vermicompost fertilizer on photosynthetic characteristics of chickpea (Cicer arietinum L.) under drought stress. Photosynthetica 54: 87-92.
Jannin, L., Arkoun, M., Etienne, P., Laıne, P., Goux, D. & Garnica, M. 2013. Brassica napus growth is promoted by Ascophyllum nodosum. Seaweed extract: microarray analysis and physiological characterization of N, C, and S metabolisms. Plant Growth Regulation 32: 31-52.
Kumar, G. & Sahoo, D. 2011. Effect of seaweed liquid extract on growth and yield of Triticum aestivum var. Pusa Gold. Journal of Apply Phycology 23: 251-255.
Lichtenthaler, H. K. & Wellburn, A.R. 1983. Determination of total carotenoids and chlorophylls a and b of leaf in different solvents. Biology and Soceity Transcription 11: 591-592.
Panahyan-e-Kivi, M., Ebadi, A., Tobeh, A. & Jamaati-e-Somarin, S.H. 2009. Evaluation of yield and yield components of lentil genotypes under drought stress. Research Journal of Environmental Sciences 3: 456-460.
Parsa, M. & Bagheri, A. 2008. Legumes. Publications Jahad University of Mashhad.
Rahbarian, R., Khavari-nejad, R., Ganjeali, A., Bagheri, A.R. & Najafi, F. 2011. Drought stress effects on photosynthesis, chlorophyll fluorescence and water. Acta Biologica Cracoviensia-Series Botanica 53: 47-56.
Ramarajan, S., Joseph, L.H. & Ganthi, A.S. 2012. Effect of seaweed liquid fertilizer on the germination and pigment concentration of soybean. Journal of Crop Science and Technology 1: 1-5.
Rasti Sani, M., Lahouti, M. & Ganjeali, A. 2014. Effect of drought stress on some morphophysiological traits and chlorophyll fluorescence of red bean seedlings (Phaseolus vulgaris L.). Iranian Journal of Pulses Resarch 5: 103-116.
Saeidi, M. & Abdoli, M. 2015. Effect of drought stress during grain filling on yield and its components, gas exchange variables, and some physiological traits of wheat cultivars. Journal of Agricaltural and Tecnology 17: 885-898.
Siva, M.A., Da Silva, J.A. & Sharma, S. 2007. Use of physiology parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal of Plant Physiology 19: 193-201.
Zhang, X.E. & Ervin, H. 2008. Impact of seaweed extract-based cytokinins and zeatin-riboside on creeping bentgrass heat tolerance. Crop Science 48: 364-370.