The impact of tarragon essential oil on the activity of starter bacteria, molds and yeasts and the improvement of the properties of traditional yogurt

Authors

Department of Microbiology and Microbial Biotechnology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran

Abstract
The aim of this study was to increase the shelf life of traditional yogurt, to improve its consistency and to prevent whey syneresis by adding tarragon essential oil. Yogurt was produced by industrial procedures and then the essential oil was inoculated into yogurt in different concentrations (10, 25, 30 ppm). Samples were examined for water storage capacity and microbial evaluation during 3 weeks of storage at two temperatures (refrigerator temperature and room temperature). The results showed that different concentrations of tarragon, temperature and storage time had a significant effect (p <0.05) on water holding capacity as well as the number of starter bacteria, molds, and yeasts. Tarragon essential oil reduced the number of bacteria, molds, and yeasts that spoil yogurt. The final results showed that the use of tarragon was not effective in water retention of yogurt samples, but it affected the number of initiator bacteria and yeast. Yogurt samples with high concentration of tarragon had the highest score in terms of consistency and sour taste. While samples with low concentration of tarragon, in terms of yogurt taste, had the highest sensory score.

Keywords


Al-Kadamany, E., Khattar, M., Haddad, T. & Toufeili, I. 2003. Estimation of shelf-life of concentrated yogurt by monitoring selected microbiological and physicochemical changes during storage. Lebensmittel-Wissenschaft & Technologie (LWT)-Food Science and Technology 36: 407-414.
Amatayakul, T., Halmos, A.L., Sherkat, F. & Shah, N.P. 2006. Physical characteristics of yoghurts made using exopolysaccharide-producing starter cultures and varying casein to whey protein ratios. International Dairy Journal 16: 40-51.
Amensour, M., Bouhdid, S., Fernández-López, J., Idaomar, M., Senhaji, N.S. & Abrini, J. 2010. Antibacterial activity of extracts of Myrtus communis against food-borne pathogenic and spoilage bacteria. International Journal of Food Properties 13: 1215-1224.
Bendall, J.G., Crawford, R.A., Evers, J.M., Smythe, B.W., Bessaire, T., Mottier, P., Mujahid, C., Aleksic, G. & Hutchinson, R. 2019. Determination of nitrofurazone in fluid milk and dairy powders. Part 1: An international pilot study. International Dairy Journal 91: 185-192.
Bonyadian, M. & Moshtaghi, H. 2008. Bacteriocidal activity of some plants essential oils against Bacillus cereus, Salmonella typhimurium, Listeria monocytogenes and Yersinia enterocolitica. Research Journal of Microbiology 3: 648-653.
Chuesiang, P., Siripatrawan, U., Sanguandeekul, R., Yang, J.S., McClements, D.J. & McLandsborough, L. 2019. Antimicrobial activity and chemical stability of cinnamon oil in oil-in-water nanoemulsions fabricated using the phase inversion temperature method. Lebensmittel-Wissenschaft & Technologie (LWT)-Food Science and Technology 110: 190-196.
Farnsworth, J., Li, J., Hendricks, G. & Guo, M. 2006. Effects of transglutaminase treatment on functional properties and probiotic culture survivability of goat milk yogurt. Small Ruminant Research 65: 113-121.
Hajiei, M., Khodaiyan, F. & Pourahmad, R. 2017. The effect of kefiran as a fat replacer on physicochemical properties, sensory and microbial stirred fruit yoghurt. Iranian Journal of Biosystems Engineering 48: 427-433.
Iranian National Standardization Organization. 1398. Yoghurt-specifications and test methods. 695. http://standard.isiri.gov.ir/StandardView.aspx?Id=52740.
Kalhori, R. 2008. The effect of using tarragon and thyme essential oils along with wheat germ powder on some properties of plain yogurt. 17th National Congress of Food Industry of Iran, Urmia University: 54-58.
Ledenbach, L.H. & Marshall, R.T. 2009. Microbiological spoilage of dairy products. In compendium of the microbiological spoilage of foods and beverages. Springer New York: 41-67.
Lu, M. & Wang, N. 2017. Chapter 7-spoilage of milk and dairy products BT-the microbiological quality of food. Woodhead Publishing Series in Food Science, Technology and Nutrition.
Mahmoudi, R., Norian, R., Pajohi-Alamoti, M.R. & Aliakbarlu, J. 2014. Effect of season on microbial and chemical characteristics of milk and yoghurt from sheep. Animal Production Science 54: 1091-1094.
Mohammadi, A., Hashemi, M. & Hosseini, S.M. 2015. Nanoencapsulation of Zataria multiflora essential oil preparation and characterization with enhanced antifungal activity for controlling Botrytis cinerea, the causal agent of gray mould disease. Innovative Food Science & Emerging Technologies 28: 73-80.
Mohammadi, A., Hosseini, S.M. & Hashemi, M. 2020. Emerging chitosan nanoparticles loading-system boosted the antibacterial activity of Cinnamomum zeylanicum essential oil. Industrial Crops and Products 155: 112824.
Najafi, M.S., Mortazavi, S.M.H. & Motamedi, H. 2019. Antimicrobial effect of ten essential oils on bacteria and yeast causing date fruit spoilage in rutab stage. Journal of Applied Microbiology in Food Industries 5: 14-27.
Noruzi, F., Hojjati, M., Jooyandeh, H. & Barzegar, H. 2018. Study of the possibility of application of tarragon essential oil in mayonnaise as a natural additive. Journal of Food Research 28: 85-99.
Seo, M.H., Chang, Y., Lee, S. & Kwak, H. 2011. The physicochemical and sensory properties of milk supplemented with ascorbic acid‐soluble nano‐chitosan during storage. International Journal of Dairy Technology 64: 57-63.
Sulaiman, S., Ibrahim, D., Kassim, J. & Sheh-Hong, L. 2011. Antimicrobial and antioxidant activities of condensed tannin from Rhizophora apiculata barks. Journal of Chemical and Pharmaceutical Research 3: 436-444.
Tamime, A.Y. & Robinson, R.K. 2007. Tamime and Robinson’s yoghurt: Science and Technology, 3rd ed. CRC Press, Cambridge, United Kingdom,pp. 759-760
Yörük, N.G. 2018. Most probable number technique in Escherichia coli count using ISO 16649‐3, ISO 7251, and rapid test enumeration device (TEMPO EC) methods in milk and dairy products. Journal of Food Safety 38: e12502.
Zedan, H., Hosseini, S.M. & Mohammadi, A. 2021. The effect of tarragon (Artemisia dracunculus) essential oil and high molecular weight chitosan on sensory properties and shelf life of yogurt. Lebensmittel-Wissenschaft & Technologie (LWT)-Food Science and Technology 147: 111613.

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026