Evaluation of sexual dimorphism on the basis of morphometric traits in Talysh toad, Bufo eichwaldi (Litvinchuk, Borkin, Skorinov & Rosanov, 2008), along with the evaluation of the conservation status of the species

Javanbakht, Hossein; Heidari, Nastaran; Alinezhadi, Iman

doi:10.52547/nbr.9.1.17

Evaluation of sexual dimorphism on the basis of morphometric traits in Talysh toad, Bufo eichwaldi (Litvinchuk, Borkin, Skorinov & Rosanov, 2008), along with the evaluation of the conservation status of the species

Authors

Hossein Javanbakht ¹

Nastaran Heidari ²

Iman Alinezhadi ¹

¹ Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

² Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Karaj, Iran

https://doi.org/10.52547/nbr.9.1.17

Abstract

The status of sexual dimorphism patterns in human and various animal groups including some mammals, birds, reptiles and amphibians have been studied, however, despite the fact that a growing number of studies were aimed to describe sexual dimorphism patterns in external morphological features such as the proportion of toes in animals, our knowledge of some basic issues, specially in some animal groups, is still limited. In this study, sexual dimorphism in terms of external morphological features as well as the ratio between finger lengths II to IV (2D: 3D, 2D: 4D, 3D: 4D) of the front and rear limbs in 30 specimens of Bufo eichwaldi toads (15 male and 15 female specimens) were in northern Iran. The results showed that females had higher mean body length (SVL) than males and this difference was statistically significant (90.50 mm in males vs. 114.93 mm in females and P = 0.00). In terms of other morphological traits, the results of T-test analysis (T test for independent samples) showed that the traits of diameter between two eyes (IOD), tympanum diameter (TD), length of the humerus (LHU), forearm length (FOL) and foot length (FL) had significant differences (P ≤ 0.05) between males and females. Also, the ratio of the size of the length of the fingers and toes on the left side of the body was examined in the species studied in terms of 2D:3D, 2D:4D, 3D:4D. The results showed that males and females were significantly different in two traits including 4^th toe length (D4) and 1^st finger length (F1). In addition, in terms of 2D:4D between males (0.4628) and females (0.4853) significant difference (P = 0.041) in the second and fourth toes of the hind limbs and in terms of 3D:4D between males (0.6233) and females (0.6787), there was a significant difference (P= 0.040) in the third and fourth fingers of the hind limbs. In this study, some habitat characteristics, ecology, threats and conservation measures related to Talysh toad species were also investigated.

Keywords

amphibian

ecology

morphology

Sustan wetland

tetrapod

20.1001.1.24236330.2022.9.1.2.2

Alinezhadi, I., Heidari N. & Javanbakht, H. 2020. Sexual dimorphism in digit length ratios of the second to fourth digits (2D:4D) in two species of green toads (Pelobates syriacus Boettger, 1889 and Bufo viridis (Laurenti, 1768)). Nova Biologica Reperta 7: 295-303. (In Persian).
Balogová, M., Nelson, E., Uhrin, M., Figurová, M. & Ledecký, V. 2015. No sexual dimorphism detected in digit ratios of the fire salamander (Salamandra salamandra). Anatomical Record 298: 1786-1795.
Beaty, L., Emmering, Q.C. & Bernal, X.E. 2016. Mixed sex effects on the second to fourth digit ratio of tungara frogs (Engystomops pustulosus) and cane toads (Rhinella marina). Anatomical Record 299: 421-427.
Brown, W.M., Finn, C.J. & Breedlove, S.M. 2002. Sexual dimorphism in digit-length ratios of laboratory mice. Anatomical Record 267: 231-234.
Burley, N.T. & Foster, V.S. 2004. Digit ratio varies with sex, egg order and strength of mate preference in zebra finches. Proceedings of Biological Science 271:239–244.
Chang, J.L. 2008. Sexual dimorphism of the second-to-fourth digit length ratio (2D:4D) in the strawberry poison dart frog (Oophaga pumilio) in Costa Rica. Journal of Herpetology 42:414–416.
De la Torre, J.G. & Sobrino, E. 2001. Dispositivospara evitar el atropello de anfibios. Quercus 183: 20–22.
Direnzo, G.V. & Stynoski, J.L. 2012. Patterns of second-to-fourth digit length ratios (2D:4D) in two species of frogs and two species of lizards at La Selva, Costa Rica. Anatomical Record 295:597–603.
Dodd, J.R. & Cade B. 1998. Movement patterns and the conservation of amphibians breeding in small, temporary wetlands. Conservation Biology 12:331-339.
Fuse, M. & Sawada, K. 2019. Morphological development of baculum and forelimb second-to-fourth digit ratio in mice. Congenital Anomalies (Kyoto) 59: 24-32.
Germano, J., Cree, A. & Bishop, P. 2011. Ruling out the boys from the girls: can subtle morphological differences identify sex of the apparently monomorphic frog, Leiopelma pakeka? New Zealand Journal of Zoology 38: 161-171.
Gomes, C.M. & Kohlsdorf, T. 2011. Evolution of sexual dimorphism in the digit ratio 2D:4D - relationships with body size and microhabitat use in iguanian lizards. PLoS One 6:e28465.
Heidari, N. 2021. Sexual dimorphism in digit length ratios of the second to fourth digits (2D:4D) in Blanford's fringe- toed lizard Acanthodactylus blanfordi Boulenger, 1918 (Sauria: Lacertidae) in southern Iran. Nova Biologica Reperta 8: 46-55. (In Persian).
Heidari, N., & Rastegar-Pouyani, N. 2010. A new record of the Baluchistan coastal toad Bufo olivaceus Blanford, 1874 (Anura: Bufonidae) from southeastern Iran. Russian Journal of Herpetology 17: 243-244.
Kaczmarski, M., Kubicka, A.M., Tryjanowski, P. & Hromada, M. 2015. Females have larger ratio of second-to-fourth digits than males in four species of Salamandridae, Caudata. Anatomical Record, 298: 1424-1430.
Kaczmarski, M., Ziemblińska, K. & Tryjanowski, P. 2020. Sand lizards Lacerta agilis with higher digit ratios are more likely to autotomy. Journal of Anatomy 237: 1103-1113.
Kami, H.G. & Yadollahvand, R. 2014. First Karyological study of the Talysh toad (Bufo eichwaldi) in Mazandaran province, Iran (Anura: Bufonidae). Bulletin of Environment, Pharmacology and Life Sciences 3: 75-78.
Kazimirski, P.P., Kaczmarski, M., Zagalska-Neubauer, M.M., Żołnierowicz, K.M., Tobółka, M. & Kazimirski, PP. 2019. Absence of sex differences in digit ratio in nestlings of the white stork Ciconia ciconia, a monomorphic bird species. Bird Study 66: 503-509.
Kaczmarski, M., Kaczmarek, M., Jankowiak, L., Kolenda, K. & Tryjanowski, P. 2021. Digit ratio in the common toad Bufo bufo: the effects of reduced fingers and of age dependency. Zoological Letters 7: 1-15.
Leoni, B., Rubolini, D., Romano, M., DiGiancamillo, M. & Saino, N., 2008. Avianhindlimb digit length ratios measured from radiographs are sexually dimorphic Journal of Anatomy 213: 425-430.
Leoni, B., Canova, L. & Saino, N. 2005. Sexual dimorphism in metapodial and phalanges length ratios in the wood mouse. Anatomical Record 286: 955-961.
Litvinchuk, S.N., Borkin, L.J., Skorinov, D.V., Rosanov, J.M. 2008. A new species of common toads from the Talysh mountanis, south-eastern Caucasus: genomic size, allozyme, and morphological evidences. Russian Journal of Herpetology 15: 19-43.
Lofeu, L., Brandt, R. & Kohlsdorf, T. 2017. Phenotypic integration mediated by hormones: associations among digit ratios, body size and testosterone during tadpole development. BMC Evolutionary Biology 17: 175-182
Lofeu, L., Brandt, R. & Kohlsdorf, T. 2020. Digit identity matters: origin and evolution of sexual dimorphism in the digit lengths of tropidurid lizards. Biological Journal of Linnaean Society 31: 109-121.
Manning, JT. 2002. Digit ratio. A pointer to fertility, behavior, and health. New Brunswick, New Jersey, London: Rutgers University Press; Pp: 175.
Manning, JT., Scutt, D., Wilson, J. & Lewis-Jones, DI. 1998. The ratio of 2nd to 4th digit length: a predictor of sperm numbers and concentrations of testosterone, luteinizing hormone and oestrogen. Human Reprodution 13: 3000-3004.
Marczak, M., Misiak, M., Sorokowska, A. & Sorokowski, P. 2018. No sex difference in digit ratios (2D:4D) in the traditional Yali of Papua and its meaning for the previous hypotheses on the inter-populational variability in 2D:4D. American Journal of Human Biology 30: 4-7.
Marshall, I., Corner, A. & Tattersfield, P., 1997.An amphibian mitigation scheme for the A34 Wilmslow and Handforth Bypass, Chesire, United Kingdom. In: Habitat fragmentation and infra-structure 5: 227-237
McIntyre, M., Herrmann, E., Wobber, V., Halbwax, M., Mohamba, C. & de Sousa N. 2009. Bonobos have a more human-like second-to-fourth finger length ratio (2D:4D) than chimpanzees: a hypothesized indication of lower prenatal androgens. Journal of Human Evolution 56: 361-365.
Mohammadpour Hardani, D. 2020. Skeletal chronological examination of the variable toad Bufotes varibilis (amphibian: Bufonide) in Khorramshahr and Yasuj regions. Master Thesis in Animal Biosystematics, Yasouj University (In Persian).
Orlowski, G. 2007. Spatial distribution and seasonal pattern in road mortality of the common toad Bufo bufo in an agricultural landscape of south–western Poland. Amphibia–Reptilia 28: 25-31.
Rajabi, F. & Javanbakht, H. 2019. Sexual dimorphism in digit length ratios in marsh frog, Pelophylax ridibundus (Ranidae) from Iran. Journal of Applied Biological Science 13: 33-36.
Ribeiro, E., Neave, N., Morais, RN. & Manning, JT. 2016. Direct versus indirect measurement of digit ratio (2D:4D): a critical review of the literature and new data. Evolutionary Psychology 14: 1-8.
Rosell, C., Parpal, J., Campeny, R., Jové, S., Pasquina, A. & Velasco, JM. 1997. Mitigation of barrier effect of linear infrastructures on wild-life. In: Habitat fragmentation and infrastructure: 6: 367-371
Rosell, C. & Velasco, J.M., 1999. Manual deprevenció i correcció dels impactes de lesinfraestructures viàries sobre la fauna. Docu-ments dels Quaderns de Medi Ambient 4: 1-95.
Rubolini, D., Pupin, F., Sacchi, R., Gentili, A., Zuffi, MA. & Galeotti, P. 2006. Sexual dimorphism in digit length ratios in two lizard species. Anatomical Records 288: 491-497.
Tolosa, Y., Molina-Zuluaga, C., Restrepo, A. & Daza J. 2014. Sexual maturity and sexual dimorphism in a population of the rocket-frog Colostethus aff. fraterdanieli (Anura: Dendrobatidae) on the northeastern Cordillera Central of Colombia. Actual Biology 37: 287-294.
Van Damme, R., Wijnrocx, K., Boeye, J., Huyghe, K. & Van Dongen, S. 2015. Digit ratios in two lacertid lizards: sexual dimorphism and morphological and physiological correlates. Zoomorphology 134: 565-575.
Woodhead, N., Hare, KM. & Cree, A. 2018. Sexual dimorphism of digit-length ratio in a viviparous lizard: influence of age, but not preservation state or sex of intrauterine twin. Anatomical Records 301: 1169-1178.
Yousefi, S.S., Saeedi, H., Sadat Behfar M., Fallahi R., & Izadian M. 2013. Atlas of Amphibians of Iran. Deputy of Natural Environment Division, Department of Environment of Iran, pp: 112.