Nova Biologica Reperta

Kinetic study of peroxidase enzyme in the presence of purine alkaloids

Authors

Tayyebeh Rahmati darvazi
Reyhaneh Sariri

Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

https://doi.org/10.52547/nbr.8.4.279
Abstract
Peroxidase catalyzes different oxidation of substrates using hydrogen peroxide, a reactive oxygen specie (ROS). ROS, at low concentrations, act as messenger to regulate intracellular signaling, whereas, at high concentrations, they can overcome the immune system by creating oxidative stress. Some common beverages such as coffee, tea and soft drinks contain high levels of xanthine alkaloids including theophylline and theobromine. In this study, the effect of theophylline and theobromine on peroxidase activity was kinetically studied by measuring the absorption of 4-aminoantipyrine, oxidized in the presence and absence of theophylline and theobromine at 510 nm for 3 minutes. The results showed that theobromine and theophylline acted as inhibitors with IC50 of 0.50 and 0.55 mM, respectively. Km and Vmax values showed that both compounds are non-competitive inhibitors. The values of Ki were calculated as 0.03 and 0.045 mM for theobromine and theophylline, respectively. Lower values of Ki and IC50 for theobromine compared to theophylline indicates that theobromine has a higher inhibition strength and binding tendency to the enzyme-substrate complex. Hence, it is concluded that theobromine has a stronger inhibitory effect on POD activity.


Keywords

antioxidant
free radical
inhibitor
theobromine
theophylline
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Nova Biologica Reperta
Volume 8, Issue 4 - Serial Number 30
Autumn 2021
Pages 279-288

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026

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