Analyzing the association of MMP-3 promoter polymorphism and its serum concentration with the outcome of in vitro fertilization and embryo transfer

Meraji Masouleh Moghaddam, Mahdis; Mashayekhi, Farhad; Zahiri, Ziba; Eidi, Akram

doi:10.52547/nbr.8.4.242

Analyzing the association of MMP-3 promoter polymorphism and its serum concentration with the outcome of in vitro fertilization and embryo transfer

Authors

Mahdis Meraji Masouleh Moghaddam ¹

Farhad Mashayekhi ²

Ziba Zahiri ³

Akram Eidi ¹

¹ Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

² Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

³ Reproductive Health Research Center, Department of Obstetrics & Gynecology, Alzahra Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

https://doi.org/10.52547/nbr.8.4.242

Abstract

This study aimed to investigate the polymorphism of matrix metalloproteinase -3 (MMP-3) gene and its expression in the serum of infertile female patients received in vitro fertilization and embryo transfer (IVF-ET). To do so, 100 women with unsuccessful IVF-ET (IVF^–) and 100 women with successful IVF-ET procedure and clinical pregnancy (IVF⁺) were included. Genetic polymorphism and serum concentration of MMP3 were investigated by ARMS-PCR and ELISA, respectively. The results showed no significant association between MMP-3 gene polymorphism and IVF-ET outcome among the two groups studied. However, a significant decrease in the concentration of MMP-3 serum in the IVF^– group was observed in comparison with the IVF⁺group (P=0.000002). Moreover, we showed that the serum MMP-3 levels in CC, AC and AA genotypes in the IVF^– group were 33, 65.33 and 86 ng/ml, respectively. In conclusion, while there is no significant difference between MMP-3 promoter polymorphism and IVF-ET outcome between the IVF⁺ and IVF^- groups, a significant decrease in MMP-3 serum levels in IVF^- group was seen as compared with the IVF⁺ group. It could be also suggested that the CC genotype is associated with a decreased level of MMP-3 serum concentration and may be associated with IVF-ET failure.

Keywords

clinical pregnancy

genetic variation

implantation failure

Infertility

rs632478

20.1001.1.24236330.1400.8.4.6.1

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