The effects of copper nanoparticles and cold atmospheric plasma on biochemical indices of Dracocephalum moldavica

Haddadian, Kazhal; Iranbakhsh, Alireza; Khavari-Nejad, Ramazan Ali; Ghoranneviss, Mahmood

doi:10.52547/nbr.7.4.411

The effects of copper nanoparticles and cold atmospheric plasma on biochemical indices of Dracocephalum moldavica

Authors

Kazhal Haddadian ¹

Alireza Iranbakhsh ¹

Ramazan Ali Khavari-Nejad ¹

Mahmood Ghoranneviss ²

¹ Department of Biology, Faculty of Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

² Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran

https://doi.org/10.52547/nbr.7.4.411

Abstract

The Moldavian dragonhead (Dracocephalum moldavica L., Lamiaceae) is an annual medicinal plant with beneficial nutritional sources that plays important roles in human and animal feed. Nanoparticles and cold atmospheric plasma increase biochemical compounds in plants. In this study, the effects of copper nanoparticles and cold atmospheric plasma on biochemical indices of the medicinal plant Dracocephalum moldavica were investigated. Moldavian dragonhead plants were subjected to four doses of copper nanoparticles (0, 25, 50 and 75 mgl^-1) and cold atmospheric plasma at three durations (zero, 20 and 30 s). The results showed that cold atmospheric plasma significantly increases the essential oil percentage, while it decreases the amount of flavonoid content and activity of catalase and peroxidase enzymes. Cold atmospheric plasma (20 s) showed significant positive impact on essential oil content, while different time duration (20 and 30 s) did not show a significant impact on other traits. Lower doses of copper nanoparticles (25 and 50 mgl^-1) showed positive impacts on measured traits, while 75 mgl^-1 dose negatively affected the measured traits and functioned as a heavy metal. The cold atmospheric plasma and copper nanoparticles interactions indicated that cold atmospheric plasma had an incremental effect on the improvement of measured traits and increased the effect of copper nanoparticles. In conclusion, the results showed that copper nanoparticles with 25 mgl^-1 dose along with cold atmospheric plasma with 20 s duration had significant positive effects on the improvement of biochemical indices of Dracocephalum moldavica.

Keywords

Catalase

essential oil percentage

flavonoids

Lamiaceae

Peroxidase

20.1001.1.24236330.1399.7.4.4.2

Abd El-Baky, H. & El-Baroty, G. 2008. Chemical and biological evaluation of the essential oil of Egyptian moldavian balm (Dracocephalum moldavica L.). International Journal of Integrative Biology 3: 202-208.
Aebi, H. 1984. Catalase in vitro. Methods Enzymology 105: 121-126.
Asgari-Targhi, G., Iranbakhsh, A. & Ardebili, Z.O. 2018. Potential benefits and phytotoxicity of bulk and nano-chitosan on the growth, morphogenesis, physiology, and micropropagation of Capsicum annuum. Plant Physiol. Biochem. 127: 393-402.
Baruah, S. & Dutta, J. 2009. Nanotechnology applications in pollution sensing. and degradation in agriculture: a review. Environm. Chem. Lett. 7: 191-204.
Bauder, T. & R, Waskom. 2003. Best management practices for colorado corn. Colorado State Univ. Coop. Ext. Bul. Xcm 574a. Fort Collins, Co. 87p.
Bormashenko, E., Shapira, Y., Grynyov, R., Whyman, G., Bormashenko, Y. & Drori, E. 2015. Interaction of cold radiofrequency plasma with seeds of beans (Phaseolus vulgaris). J. Exp. Botany 66: 4013-4021.
Chandra, S., Kumar, A. & Tomar, P. K. 2014. Synthesis and characterization of copper nanoparticles by reducing agent. J. Saudi Chem. Soci. 18: 149-153.
Choudhary, M., Jetley, U. K., Khan, M. A., Zutshi, S. & Fatma, T. 2007. Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium spirulina platensis-s5. Ecotoxicol. Environm. Safety 66: 204-209.
Costa, M.V.J. & Sharma, P.K. 2016. Effect of copper oxide nanoparticles on growth, morphology, photosynthesis, and antioxidant response in Oryza sativa. Photosynthetica 54: 110-119.
Dai, L.P., Xiong, Z.T., Huang, Y. & Li, M.J. 2006. Cadmium‐induced changes in pigments, total phenolics, and phenylalanine ammonia‐lyase activity in fronds of azolla imbricata. Environm. Toxicol. Journal 21: 505-512.
Fernández, A. & Thompson, A. 2012. The inactivation of salmonella by cold atmospheric plasma treatment. Food Res. Inter. 45: 678-684.
Haddadian K., Iranbakhsh, A., Khavary Nejad, R.A. & Ghorannevi, M. 2017. Copper nanoparticles together with cold atmospheric plasma improves the growth and physiological indices of Dracocephalum moldavica in hydroponic system. J. Exp. Biol.7: 130-139
Hafeez, A., Razzaq, A., Mahmood, T. & Jhanzab, H.M. 2015. Potential of copper nanoparticles to increase growth and yield of wheat. J. Nanosci. Adv. Technol. 1: 6-11.
Hussein, M.S., El-sherbeny, S.E., Khalil, M.Y. & Naguib, N.Y., Aly, S.M. 2006 . Growth characters and chemical constituents of Dracocephalum moldavica L. plants in relation to compost fertilizer and planting distance. Scientia Horticulturae 108: 322-331.
Jiang, J., Lu, Y., Li, Y., Li, L., He, X., Shao, H. & Dong, Y. 2014 . Effect of seed treatment by cold plasma on the resistance of tomato to ralstonia solanacearum. Plos One 9: E97753.
Kar, M. & Mishra, D. 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiol. 57: 315-319.
Manikandan, R., Ezhili, N. & Venkatachalam, P. 2016. Phosphorus supplementation alleviation of the cadmium-induced toxicity by modulating oxidative stress mechanisms in vetiver grass [Chrysopogon Zizanioides (L.) Roberty]. J. Environ. Engin. 142: C4016003.
Manikandan, R., Sahi, S. & Venkatachalam, P. 2015. Impact assessment of mercury accumulation and biochemical and molecular response of mentha arvensis: a potential hyperaccumulator plant. Sci. World J. 2015: 715217.
Meiqiang, Y., H. Mingjing., Buzhou, M. & Tengcai, M. 2005. Stimulating effects of seed treatment by magnetized plasma on tomato growth and yield. Plasma Scie. Tech. 7: 3143-3147
Menard, A., Drobne, D. & Jemec, A. 2011. Ecotoxicity of nanosized Tio2. Review of in vivo data. Environmental Pollution 159: 677-684.
Mihai, A., Dobrin, D., Magureanu, M. & Popa, M. 2014. Positive effect of non-thermal plasma treatment on radish seeds. Romanian Rep. Physics 66: 1110-1117.
Monica, R.C. & Cremonini, R. 2009. Nanoparticles and higher plants. Caryologia 62: 161-165.
Monreal, C. 2010. In summary of information currently provided to MRI concerning applications for Round 5 of the Ontario Research Fund-Research Excellence Program, pp.12-13
Olszewska, M., Grzegorczyk, S., Alberski, J., Baluch-Malecka, A. & Kozikowski, A. 2008. Effect of copper deficiency on gas exchange parameters, leaf greenness [spad] and yield of perennial ryegrass [Lolium perenne l.] and orchard grass [Dactylis glomerata l.]. Journal of Elementology 13: 597-604.
Pande, P., Anwar, M., Chand, S., Yadav, V.K. & Patra, D. 2007. Optimal level of iron and zinc in relation to its influence on herb yield and production of essential oil in menthol mint. Communications in Soil Science and Plant Analysis 38: 561-578.
Pandey, N., Pathak, G.C., Pandey, D.K. & Pandey, R. 2009. Heavy Metals, Co, Ni, Cu, Zn And Cd, produce oxidative damage and evoke differential antioxidant responses in spinach. Brazilian J. Plant Physiol. 21: 103-111.
Posmyk, M., Janas, K. & Kontek, R. 2007. Effect of anthocyanin-rich red cabbage extract on cytological injury induced by copper stress in plant and animal tissues. Ochrona Środowiska I Zasobów Naturalnych. 33: 50-56.
Posmyk, M., Kontek, R. & Janas, K. 2009. Antioxidant enzymes activity and phenolic compounds content in red cabbage seedlings exposed to copper stress. Ecotoxic. Environm. Safety 72: 596-602.
Rico, C.M., Majumdar, S., Duarte-Gardea, M., Peralta-Videa, J.R. & Gardea-Torresdey, J.L. 2011. Interaction of nanoparticles with edible plants and their possible implications in the food chain. J. Agricul. Food Chem. 59: 3485-3498.
Shalaby, T.A., Bayoumi, Y., Abdalla, N., Taha, H., Alshaal, T., Shehata, S., Amer, M., Domokos-Szabolcsy, É. & El-Ramady, H. 2016. Nanoparticles, soils, plants and sustainable agriculture. Nanosci. Food Agricult. 1: 283-312.
Sivachandiran, L. & Khacef, A. 2017. Enhanced seed germination and plant growth by atmospheric pressure cold air plasma: combined effect of seed and water treatment. RSC Advances 7: 1822-1832.
Song, G., Hou, W., Gao, Y., Wang, Y., Lin, L., Zhang, Z., Niu, Q., Ma, R., Mu, L. & Wang, H. 2016. Effects of Cuo nanoparticles on Lemna minor. Botanical Studies 57: 1-8
Street, R. 2012. Heavy metals in medicinal plant products—an african perspective. South African J. Bot. 82: 67-74.
Tiwari, K., Dwivedi, S., Mishra, S., Srivastava, S., Tripathi, R., Singh, N. & Chakraborty, S. 2008. Phytoremediation efficiency of portulaca tuberosa rox and portulaca oleracea l. Naturally growing in an industrial effluent irrigated area in vadodra, gujrat, india. Environmental Monitoring and Assessment 147: 15-22
Tripathi, D.K., Singh, S., Singh, S., Srivastava, P.K., Singh, V. P. & Singh S. 2017. Nitric oxide alleviates silver nanoparticles (agnps)-induced phytotoxicity in Pisum sativum seedlings. Plant Physiol. Biochem.110: 167-177.
Wang, F., Zeng, B., Sun, Z. & Zhu, C. 2009. Relationship between proline and hg 2+-induced oxidative stress in a tolerant rice mutant. Arch. Environ. Contam. Toxicol. 56: 723-731.
Wang, M.H., Misra, R.P., Giraldo, J.P., Kwak, S.Y., Son, Y. & Landry, M.P. 2016. Lipid exchange envelope penetration (LEEP) of nanoparticles for plant engineering: a universal localization mechanism. Nano Lett. 16: 1161-1172.
Wu, Z., Chi, L., Bian, S. & Xu, K. 2007. Effects of plasma treatment on maize seeding resistance. J. Maize Sci. 15: 111-113.
Zuverza-Mena N., Armendariz, R., Peralta-Videa, J.R. & Gardea-Torresdey, J.L. 2017. Effects of silver nanoparticles on radish sprouts: root growth reduction and modifications in the nutritional value. Front. Plant Sci. 7: 1-11
Zhu, H., Han, J., Xiao, Q. & Jin, Y. 2008. Uptake, translocation, and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. Journal of Environmental Monitoring 10: 713-717.