Hepatoprotective activity of Silver Nanoparticles synthesized using aqueous leaf extract of Punica granatum against induced hepatotoxicity in rats

Kumar, Manoj; Ranjan, Rakesh; Kumar, Amar; Sinha, Manoranjan Prasad; Srivastava, Rohit; Subarna, Sweta; Kumar Mandal, Samir

doi:10.52547/nbr.7.4.381

Hepatoprotective activity of Silver Nanoparticles synthesized using aqueous leaf extract of Punica granatum against induced hepatotoxicity in rats

Authors

Manoj Kumar ¹

Rakesh Ranjan ²

Amar Kumar ²

Manoranjan Prasad Sinha ²

Rohit Srivastava

Sweta Subarna

Samir Kumar Mandal

¹ Department of Zoology, St. Xavier’s College, Ranchi – 834001, Jharkhand, India

² Department of Zoology, Ranchi University, Ranchi – 834008, Jharkhand, India

https://doi.org/10.52547/nbr.7.4.381

Abstract

Punica granatum leaf extracts have been used since time immemorial in traditional medicines. It is used for its antioxidant properties. Green nanoparticle synthesis is an emerging field which has opened an entirely different scope for medicinal formulations. It has been reported by many users that the green nanoparticles are more effective medicines as compared with their simple extracts. Thus, in order to evaluate these speculations, the present work was undertaken to assess the hepatoprotective activity of silver nanoparticles synthesized using aqueous leaf extract of Punica granatum in comparison with the aqueous extract. After CCl₄ intoxication the serum bilirubin total increased significantly (p<0.05) and the total protein level decreased significantly (p<0.05) as compared with the control group; in addition, alkaline phosphatase activity, aspartate aminotransferase activity and alanine transaminase activity increased significantly (p<0.05). The CCl₄ intoxicated rats were treated with aqueous leaf extract and synthesized nanoparticles, the results clearly revealed that the aqueous extract of Punica granatum showed hepatoprotective effect, as the liver profile altered by CCl₄ toxicity, recovered to normal control values. Moreover, the nanoparticles synthesized using aqueous leaf extract of Punica granatum were comparatively more effective as hepatoprotective agent than the aqueous extract of Punica granatum.

Keywords

ALP

ALT

AST

bilirubin

CCl4

liver profile

Total protein

20.1001.1.24236330.1399.7.4.7.5

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