جذب زیستی رنگ آزو کنگورد توسط قارچ Mucor circinelloides و کاربرد آن در رنگ‌بری زیستی پساب صنایع نساجی

نویسندگان

بخش زیست فناوری میکروبی، دانشکده زیست شناسی، پردیس علوم، دانشگاه تهران، تهران، ایران

چکیده
سالانه مصرف گسترده رنگ­ها در صنایع نساجی و تخلیه پساب­ها به محیط منجر به آلودگی­های وسیع زیست­محیطی می­شود؛ بنابراین تصفیه این پساب­ها با استفاده از روش­های کارآمد و دوست­دار محیط­زیست ضروری است. در این مطالعه، جداسازی سویه­های توانمند با روش غنی­سازی صورت ­پذیرفت و با توجه به بیشترین میزان جذب رنگ در طول­موج ­500­ نانومتر در کمترین زمان، بهترین جدایه انتخاب و در غلظت‌های مختلف از کنگورد مورد بررسی رنگ‌بری قرار گرفت. در این مطالعه 50 گونه قارچی جداسازی شد که 10­ جدایه دارای ویژگی رنگ­بری بود. بر اساس نتایج جدایه ADH8­ با جذب 94 ­درصد از رنگ به عنوان قارچ برتر انتخاب شد. علاوه­ بر ­این، طی 48 ساعت 80­ درصد از رنگ در غلظت­­های مختلف توسط این جدایه از محیط­کشت حذف و بیشترین میزان رشد و حذف رنگ در غلظت 1000 میلی­گرم ­بر ­لیتر به­دست آمد. همچنین نتایج حاکی از آن بود که غلظت­های مختلف نمک، تأثیری بر میزان جذب رنگ توسط جدایه منتخب ندارد. شناسایی مولکولی توالی ITS جدایه ADH8 مشخص کرد که این جدایه به میزان 100­ درصد مشابه Mucor circinelloides بوده و با کد شناسایی UTMC 5032 در کلکسیون میکروارگانیسم‌های دانشگاه تهران ثبت و نگهداری گردید. نتایج حاصل از میزان رنگ­بری پساب واقعی صنایع رنگرزی نشان داد که بیشترین میزان جذب رنگ توسط UTMC 5032­M. circinelloides 35-60 درصد طی تیمار 3 ساعته زیست‌توده با پساب رنگرزی نسبت به نمونه شاهد بود. نتایج به‌دست‌آمده نشان داد UTMC 5032­M. circinelloides توانایی بالایی در جذب رنگ‌های آزو داشته و برای اولین بار جهت جذب پساب­های صنایع نساجی مورد استفاده قرارگرفته است.

کلیدواژه‌ها


عنوان مقاله English

The biosorption of Congo red azo dye by fungus Mucor circinelloides and its application in the decolorization of textile industry wastewater

نویسندگان English

Ehsan Azin
Hamid Moghimi
University of Tehran
چکیده English

The extensive application of dyes in the textile industries and their discharge in the wastewaters leads to numerous environmental pollutions; therefore, treating these wastewaters by efficient and eco-friendly methods is a necessity. In this study, potent strains were isolated by the enrichment technique according to their maximum dye sorption at the lowest possible time at 500nm. Consequently, the best isolate was selected and the dye removal was investigated in different concentrations of Congo red. Therefore, 50 different fungal strains were isolated in this study, of which 10 were able to dye removal. According to the results, isolate ­ADH8 was selected as the best strain with 94% of dye sorption. Moreover, during 48 hours, 80% of dye content was removed at all dye concentrations by this isolate, and the most growth rate and dye removal was achieved at 1000­ mg/l. The results showed that different salt concentrations have no effect on dye sorption of the selected isolate. Molecular identification of ADH8 revealed that this isolate have a 100% similarity to Mucor circinelloides which was deposited under the accession number of UTMC­5032 in the University of Tehran Microorganisms Collection. The results obtained from the dye removal of textile wastewater showed that the most amount of dye sorption by M. circinelloides UTMC­5032­ was 35-60% during three ­hours of biomass treatment as compared with the control sample. The obtained results indicated that, M. circinelloides UTMC­5032 is highly capable in azo dyes sorption and could be utilized in the biosorption of dye in the textile industries wastewaters for the first time.


کلیدواژه‌ها English

biotreatment
dyeing effluents
isolation
myco-sorbent
screening
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405