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شناسایی مولکولی کوکسیلا بورنتی در کنه‌های جدا شده از بزهای شهرستان مشکین شهر، استان اردبیل، ایران

نویسندگان

بیژن اسمعیل نژاد 1
جمال قره خانی 2
آوات سمیعی 1
هادی رضایی 1

1 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

2 گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران؛ سازمان دامپزشکی کشور، همدان، ایران

https://doi.org/10.52547/nbr.7.3.315
چکیده
کوکسیلا بورنتی عامل تب‌کیو است که بیش از 40 گونه کنه در انتقال آن نقش دارند. هدف از مطالعه حاضر بررسی گونه‌های کنه سخت در گردش بزهای شهرستان مشکین شهر در استان اردبیل و نقش آن‌ها در انتقال کوکسیلا بورنتی بود. در یک نمونه‌گیری تصادفی از فروردین 1394 لغایت فروردین 1395، تعداد 365 بز از نظر آلودگی به کنه‌های سخت مورد بازرسی قرار گرفتند. تعداد 280 کنه جمع‌آوری شده از سطح بدن دام‌ها مورد شناسایی قرار گرفته و از نظر آلودگی به کوکسیلا بورنتی به روش مولکولی بررسی شدند. 8/40 درصد از دام‌ها حداقل به یک کنه آلوده بودند. هیالوما آناتولیکوم آناتولیکوم (9/33 درصد)، ریپی‌سفالوس سانگوئینوس (1/22 درصد)، ریپی‌سفالوس تورانیکوس (1/17 درصد)، هیالوما اکسکاواتوم (1/11 درصد)، ریپی‌سفالوس بورسا (5 درصد)، هیالوما دتریتوم (9/3 درصد)، هیالوما درومداری (6/3 درصد)، هیالوما اسیاتیکوم اسیاتیکوم (8/1 درصد) و هیالوما مارژیناتوم (1 درصد) گونه‌های شناسایی‌شده هستند. اختلاف آماری معنی‌داری بین میزان آلودگی دام‌ها به کنه و فصول مختلف، جنس و سن آن‌ها مشاهده نشد (05/0=p). در بررسی مولکولی 280 کنه صید شده، 5 مورد از 40 دسته شامل هیالوما آناتولیکوم آناتولیکوم (3 pool)، هیالوما اکسکاواتوم (1 pool) و ریپی‌سفالوس سانگوئینوس (1 pool) آلوده به کوکسیلا بورنتی بودند. بررسی حاضر اولین گزارش از وضعیت آلودگی کنه‌ها به کوکسیلا بورنتی در شمال‌غرب ایران است. با توجه به زئونوز بودن تب‌کیو، مطالعه بیشتر در مورد ناقلین و میزبان‌های دیگر ضروری به‌نظر می‌رسد.








کلیدواژه‌ها

بز
تب‌کیو
شمال‌غرب ایران
کنه سخت
واکنش زنجیره پلی‌مراز

عنوان مقاله English

Molecular detection of Coxiella burnetii in ticks isolated from goats of Meshkin-Shahr County, Ardabil Province, Iran

نویسندگان English

Bijan Esmaeilnejad 1
Jamal Gharekhani 2
Awat Samiei 1
Hadi Rezaei 1
چکیده English

Q fever is caused by Coxiella burnetii and is transmitted by more than 40 tick species. This study aimed to identify the tick species in goats of Meshkin-Shahr, Ardabil Province, Iran, and their role in the transmission of C. burnetii. A number of 365 goats were inspected for the infestation of hard ticks in a randomized sampling design, during a 1-year period from April 2013 to April 2014. A total number of 280 tick specimens were collected and identified and were then examined by molecular methods for the presence of C. burnetii infection. 40.8% of the infected animals had at least one species of tick. The goats which were studied were found to be infested by the following tick species: Hyalomma anatolicum anatolicum (33.9%), Rhipicephalus sanguineus (22.1%), Rhipicephalus turanicus (17.1%), Hyalomma excavatum (11.1%), Rhipicephalus bursa (5%), Hyalomma detritum (3.9%), Hyalomma dromedarii (3.6%), Hyalomma asiaticum asiaticum (1.8%), and Hyalomma marginatum (1%). There were no significant differences between the infestation rate in different seasons, genders and ages of the animals which were studied (p=0.05). The molecular assay of 280 tick specimens revealed the presence of C. burnetii infection in 5 out of 40 pool samples (6-8 tick individuals of the same species in each pool), including H. anatolicum anatolicum (3 pools), H. excavatum (1 pool) and R. sanguineus (1 pool). The present study, therefore, reports the rate of C. burnetii infection transmitted by hard ticks in North-West of Iran for the first time. According to the zoonotic aspect of Q fever, further studies on the carriers as well as other hosts of the infection were found to be necessary.

کلیدواژه‌ها English

goat
hard tick
north-east Iran
PCR
Q fever
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

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