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اثر ضد میکروبی عصاره آبی سه گونه مریم­ گلی بر باکتری­های استافیلوکوکوس ­اورئوس، اشرشیاکولی و سودوموناس آئروجینوزا

نویسندگان

اصغر مصلح آرانی 1
نوید نعمتی 1
هنگامه زندی 2
مصطفی نادری 3

1 دانشگاه یزد

2 دانشگاه شهید صدوقی

3 دانشگاه ایلام

https://doi.org/10.29252/nbr.6.4.446
چکیده
در این مطالعه اثر عصاره آبی سه گونه Salvia perspolitana ، S. palaestina و S. bracteata بر باکتری­ های بیماری­ زای Staphylococcus aureus، Escherichia coli وPseudomonas aeroginosa تحت مطالعه قرار گرفت. جهت بررسی خاصیت ضدمیکروبی عصاره این گیاهان از روش ­های انتشار چاهک-پلیت و حداقل غلظت بازدارندگی رشد (MIC) استفاده شد. نتایج نشان داد که فقط عصاره S. bracteata در پلیتStaphylococcus aureus هاله ­عدم­ رشد به ­قطر 9 میلی­متر تشکیل داد. عصاره هر سه گیاه در Escherichia coli و Pseudomonas aeroginosa هاله ­عدم­ رشد تشکیل دادند. تاثیر عصاره S. bracteata با تشکیل هاله عدم رشد به ­قطر 10 میلی ­متر از سایر گونه­ ها مؤثر­تر بود. نتایج MIC نشان داد که عصاره S. perspolitana اثر ضعیفی بر باکتری Staphylococcus aureus داشت و اثر بازدارندگی در غلظت µg/ml 1024 مشاهده شد. عصاره این گونه، همچنین در غلظت µg/ml 256 بر باکتری­های Escherichia coli و Pseudomonas aeroginosa اثر بازدارندگی داشت. بیش ­ترین اثر برای S. bracteata به دست آمد به­ طوری که اثر بازدارندگی عصاره این گیاه برای Staphylococcus aureus، µg/ml 64 و برای دو باکتری دیگر برابر با µg/ml128 به ­دست آمد. عصاره S. palaestina اثر ضعیفی بر باکتری Staphylococcus aureus داشت و اثر بازدارندگی در غلظت µg/ml 1024 مشاهده شد. عصاره این گونه، همچنین در غلظت µg/ml 512 بر باکتری ­های Escherichia coli و Pseudomonas aeroginosa اثر بازدارندگی داشت. بنابراین نتیجه ­گیری شد که گونهS. bracteata می­ تواند به منزله گونه ­ای مناسب جهت استفاده ضد باکتریایی تحت مطالعه بیش تر قرار گیرد.

کلیدواژه‌ها

ایلام
تیره نعنائیان
روش انتشار چاهک
غلظت بازدارندگی
ضد باکتری

عنوان مقاله English

The antibacterial activity of the water extracts of three species of Salvia on Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa

نویسندگان English

Asghar Mosleh Arany 1
Navid Nemati 1
hengame Zandi 2
Mostafa Naderi 3
1 Yazd University
2 shahid sadoughi university
3 ilam university
چکیده English

The aim of this study was to evaluate the antibacterial activity of the water extracts of three species of Salvia (S. perspolitana, S. palaestina, S. bracteata) on Staphylococcus aureus, Escherichia coli and Pseudomonas aeroginosa. The antibacterial activity of water extracts of the studied species on the bacterial strains was examined using well diffusion method and minimum inhibitory concentration (MIC). Results showed that only S. bracteata formed growth inhibitory zone (9 mm) on Staphylococcus aureus. The extracts of all three plants formed growth inhibitory zone on E.coli and P. aeroginosa. The extract of S. bracteata was more effective than that of the other species. Results for MIC also showed that the extracts of S. perspolitana had the lowest effect on St. aureus and its MIC was observed in a concentration of 1024 µg/ml. The extracts of this species had the inhibitory effect in a concentration of 256 µg/ml. The uppermost inhibitory effect was provided by the extract of S. bracteata, since the minimum inhibitory concentration of this species for S. aureus was equal to 64 µg/ml; and for the other two bacteria, it was equal to 128 µg/ml. The extracts of S. palaestina had the lowest effect on S. aureus and its MIC was observed in a concentration of 1024 µg/ml. The extracts of this species had an MIC equal to 512 µg/ml for the other two bacteria. It was concluded that S. bracreata could be considered a suitable species with anti-bacterial activities in future researches.




کلیدواژه‌ها English

antibacteria
Ilam
inhibitory concentration
Lamiaceae
well diffusion method
Abbasi, N., Azizi Jalilian, F., Abdi, M. and Saifmanesh, M.A. 2007. Comparative study of the antimicrobial effect of Scrophularia striata Boiss: extract and selective antibiotics against Staphylococcus aureus and Pesudomonas aeroginosa. - J. Med. Plants 1: 10-18.
Abbasi, N., Azizi Jalilian, F., Abdi, M. and Saifmanesh, M.A. 2007. Comparative study of the antimicrobial effect of Scrophularia striata Boiss: extract and selective antibiotics against Staphylococcus aureus and Pesudomonas aeroginosa. - J. Med. Plants 1: 10-18.
Amiri, H., Meshkat, A.l., Sadat, M.H., Lari Yazdi, H. and Goodarzi, A. 2006. Essential oil composition of Salvia reuterana Boiss. - Iran. J. Medl. Arom. Plants 22: 270-275.
Amiri, H., Meshkat, A.l., Sadat, M.H., Lari Yazdi, H. and Goodarzi, A. 2006. Essential oil composition of Salvia reuterana Boiss. - Iran. J. Medl. Arom. Plants 22: 270-275.
Azaz, D., Demirci, F., Satil, F., Kurkcuoglu, M. and Baser. K.H.C. 2002. Antimicrobial activity of some Satureja essential oils. - Nature 57: 817-821. [DOI:10.1515/znc-2002-9-1011]
Azaz, D., Demirci, F., Satil, F., Kurkcuoglu, M. and Baser. K.H.C. 2002. Antimicrobial activity of some Satureja essential oils. - Nature 57: 817-821. [DOI:10.1515/znc-2002-9-1011]
Azizkhani, M., Tooryan, F. and Boreiri, M. 2016. Effects of Ocimum basilicum and Salvia sclarea essential oils on Listeria monocytogenes and Aspergillus flavus in Iranian white cheese. - Iranian Food Sci. Technol. Res. J. 12: 286-295.
Azizkhani, M., Tooryan, F. and Boreiri, M. 2016. Effects of Ocimum basilicum and Salvia sclarea essential oils on Listeria monocytogenes and Aspergillus flavus in Iranian white cheese. - Iranian Food Sci. Technol. Res. J. 12: 286-295.
Burt, S. 2004. Essential oils: Their antibacterial properties and potential applications in foods. - Int. Food Microbiol. 94: 223-25. [DOI:10.1016/j.ijfoodmicro.2004.03.022]
Burt, S. 2004. Essential oils: Their antibacterial properties and potential applications in foods. - Int. Food Microbiol. 94: 223-25. [DOI:10.1016/j.ijfoodmicro.2004.03.022]
Clauditz, A., Rescch, A., Wieland. K.P., Peschel, A. and Gotz, F. 2006. Staphyloxanthin plays a role in the fitness of Staphylococcus aureus and its ability to cope with oxidative stress. - Infect. Immun. 74: 4950-4953. [DOI:10.1128/IAI.00204-06]
Clauditz, A., Rescch, A., Wieland. K.P., Peschel, A. and Gotz, F. 2006. Staphyloxanthin plays a role in the fitness of Staphylococcus aureus and its ability to cope with oxidative stress. - Infect. Immun. 74: 4950-4953. [DOI:10.1128/IAI.00204-06]
Cursoy, N., Tape, B. and Akpulat, H.A. 2012. Chemical composition and antioxidant activity of the essential oils of Salvia palaestina and S. ceratophylla. - Rec. Nat. Prod. 6: 278-287.
Cursoy, N., Tape, B. and Akpulat, H.A. 2012. Chemical composition and antioxidant activity of the essential oils of Salvia palaestina and S. ceratophylla. - Rec. Nat. Prod. 6: 278-287.
Dorman, H.J.D. and Deans, S.G. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. - J. Appl. Microbiol. 88: 308-316. [DOI:10.1046/j.1365-2672.2000.00969.x]
Dorman, H.J.D. and Deans, S.G. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. - J. Appl. Microbiol. 88: 308-316. [DOI:10.1046/j.1365-2672.2000.00969.x]
Fattahi, B., Nazeri, V., Kalantari, S. and Bonfill, M. 2014. Identification of compounds in the essential oil and quantification of flavonoids and rosmarinic acid in Salvia reuterana and Salvia palaestina Benth. - Iranian J. Medl. Arom. Pants 30: 463-475.
Fattahi, B., Nazeri, V., Kalantari, S. and Bonfill, M. 2014. Identification of compounds in the essential oil and quantification of flavonoids and rosmarinic acid in Salvia reuterana and Salvia palaestina Benth. - Iranian J. Medl. Arom. Pants 30: 463-475.
Formisano, C., Senatore, F., Arnold, N.A., Piozzi, F. and Rosselli, S. 2007. GC and GC/MS analysis of the essential oil of Salvia hierosolymitana growing wild in Lebanon. - Nat. Prod. Com. 2: 181-184. [DOI:10.1177/1934578X0700200215]
Formisano, C., Senatore, F., Arnold, N.A., Piozzi, F. and Rosselli, S. 2007. GC and GC/MS analysis of the essential oil of Salvia hierosolymitana growing wild in Lebanon. - Nat. Prod. Com. 2: 181-184. [DOI:10.1177/1934578X0700200215]
Giner, R.M., Villalba, M.L., Recio, M.C., Mañez, S., Cerdá Nicolás, M. and Ríos, J.L. 2000. Anti-inflammatory glycoterpenoids from Scrophularia auriculata. - Eur. J. Pharmacol. 389: 243-52. [DOI:10.1016/S0014-2999(99)00846-8]
Giner, R.M., Villalba, M.L., Recio, M.C., Mañez, S., Cerdá Nicolás, M. and Ríos, J.L. 2000. Anti-inflammatory glycoterpenoids from Scrophularia auriculata. - Eur. J. Pharmacol. 389: 243-52. [DOI:10.1016/S0014-2999(99)00846-8]
Haghighati, F., Jafari, S. and Momen Beitollahi, J. 2003. Comparison of antimicrobial effects of ten herbal extracts with chlorhexidine on three different oral pathogens; an in vitro study. - Hakim Med. J. 6: 6-71.
Haghighati, F., Jafari, S. and Momen Beitollahi, J. 2003. Comparison of antimicrobial effects of ten herbal extracts with chlorhexidine on three different oral pathogens; an in vitro study. - Hakim Med. J. 6: 6-71.
Hedge, I.C. 1982. Salvia L. - In: Rechinger, K.H. (ed.), Flora Iranica 150: 403-476. Akademische Druck und Verlagsanstalt, Graz.
Hedge, I.C. 1982. Salvia L. - In: Rechinger, K.H. (ed.), Flora Iranica 150: 403-476. Akademische Druck und Verlagsanstalt, Graz.
Inouya, S., Takizawa, T. and Yamaguchi, H. 2001. Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. - J. Antimicrobial Chem. 47: 565-573. [DOI:10.1093/jac/47.5.565]
Inouya, S., Takizawa, T. and Yamaguchi, H. 2001. Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. - J. Antimicrobial Chem. 47: 565-573. [DOI:10.1093/jac/47.5.565]
Kluytmans, J., Van Belkum, A. and Verbrugh, H. 1997. Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. - J. Clin. Micrbiol. 3: 505-520. [DOI:10.1128/CMR.10.3.505]
Kluytmans, J., Van Belkum, A. and Verbrugh, H. 1997. Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. - J. Clin. Micrbiol. 3: 505-520. [DOI:10.1128/CMR.10.3.505]
Magiatis, P., Skaltsounis, A., Chinou, I. and Haroutounian, S.A. 2002. Chemical composition and invitro antimicrobial activity of the essential oil of three Greek Achillea species. - Nature 57: 287-290. [DOI:10.1515/znc-2002-3-415]
Magiatis, P., Skaltsounis, A., Chinou, I. and Haroutounian, S.A. 2002. Chemical composition and invitro antimicrobial activity of the essential oil of three Greek Achillea species. - Nature 57: 287-290. [DOI:10.1515/znc-2002-3-415]
Mozaffarian V. 2008. Flora of Ilam. Farhang Moaser Publication, Tehran. 671pp.
Mozaffarian V. 2008. Flora of Ilam. Farhang Moaser Publication, Tehran. 671pp.
Mozaffarian V. 1996. Dictionary of Iranian plant names. Farhang Moaser Publication, Tehran. 700pp.
Mozaffarian V. 1996. Dictionary of Iranian plant names. Farhang Moaser Publication, Tehran. 700pp.
National Committee for Clinical Laboratory Standard. 1990. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. - Approved Standard Order M7-A2: 1-31.
National Committee for Clinical Laboratory Standard. 1990. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. - Approved Standard Order M7-A2: 1-31.
Pirnia, M., Edalatian Dovom, M.R., Tabatabaee Yazdi, F., and Shahidi, F. 2014. The antibacterial effects of the Aqueous and Ethanolic extracts of Cordia myxa fruit on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella typhi. - Qom Univ. Medl. Sci. J. 9: 39-48.
Pirnia, M., Edalatian Dovom, M.R., Tabatabaee Yazdi, F., and Shahidi, F. 2014. The antibacterial effects of the Aqueous and Ethanolic extracts of Cordia myxa fruit on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella typhi. - Qom Univ. Medl. Sci. J. 9: 39-48.
Safavi, F., Ebrahimi, P. and Mighani, H. 2013. In vitro anti-bacterial activity of root and aerial parts of Scrophularia striata on Escherichia coli, Staphylococcus aureus and Bacillus cereus. - YUMSJ 18: 603-614.
Safavi, F., Ebrahimi, P. and Mighani, H. 2013. In vitro anti-bacterial activity of root and aerial parts of Scrophularia striata on Escherichia coli, Staphylococcus aureus and Bacillus cereus. - YUMSJ 18: 603-614.
Salimpour, F., Moazooji, A., Mazhar, F. and Barzin, G. 2014. Comparative study of antibacterial properties of four species of Salvia L. as a medicinal plant. - J. Res. Medl. Sci. 37: 205-210.
Salimpour, F., Moazooji, A., Mazhar, F. and Barzin, G. 2014. Comparative study of antibacterial properties of four species of Salvia L. as a medicinal plant. - J. Res. Medl. Sci. 37: 205-210.
Sharafati-chaleshtori, R., Sharafati-chaleshtori, F., Sharafati-chaleshtori, A. and Ashrafi, K. 2010. Antimicrobial effects and evaluation of total phenols, flavonoids and flavonols contents of ethanolic extracts of Scrophularia striata. - J. Shahrekord Univ. Medl. Sci. 11: 32-37.
Sharafati-chaleshtori, R., Sharafati-chaleshtori, F., Sharafati-chaleshtori, A. and Ashrafi, K. 2010. Antimicrobial effects and evaluation of total phenols, flavonoids and flavonols contents of ethanolic extracts of Scrophularia striata. - J. Shahrekord Univ. Medl. Sci. 11: 32-37.
Walker, J.B. and Sytsma, K.J. 2007. Stamina evolution in the genus Salvia molecular phylogenetic evidence for multiple origins of the lever. - Ann. Bot. 100: 375-391. [DOI:10.1093/aob/mcl176]
Walker, J.B. and Sytsma, K.J. 2007. Stamina evolution in the genus Salvia molecular phylogenetic evidence for multiple origins of the lever. - Ann. Bot. 100: 375-391. [DOI:10.1093/aob/mcl176]

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

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