یافته های نوین در علوم زیستی

بررسی روش‌های‌ غربالگری و پروفایل آنزیمی باکتری‌های اندوفیت گیاهی

نویسندگان

سمانه خسروشاهی 1
انسیه صالح قمری 2
محمد علی آموزگار 3
پروانه صفاریان 1

1 گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه علوم سلولی و مولکولی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران

3 گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه تهران، ایران

https://doi.org/10.29252/nbr.6.4.415
چکیده
از آنجایی که در سال ­های اخیر باکتری­ های اندوفیت گیاهی کاربردهای متنوع و مفیدی در بیوتکنولوژی پیدا کرده­اند، توجه زیادی به جداسازی، شناسایی و ارزیابی این نوع میکروارگانیسم ­ها شده است. در این مطالعه با توجه به دشوار بودن سترون سازی سطوح بافت­ های گیاهی از باکتری­ های اپی­فیت، کارایی سه روش مختلف غربالگری باکتری­های اندوفیت شامل 1- استریل کردن با آب ژاول، 2- استریل کردن متناوب (تندال تغییر یافته) و 3- استریل کردن با تریتون X100 و آب ژاول مورد ارزیابی قرار گرفت. روش تندال تغییر یافته روشی ابداعی است که در این پژوهش به منظور حذف مناسب اسپور­های درونی باکتری ­های اپی فیت که در جداسازی اندوفیت­ ها مانع به حساب می ­آیند، به کار رفت. بیش ­ترین تعداد باکتری­ های اندوفیت از گیاهان دولپه ­ای و از برگ جداسازی شدند. همچنین باکتری­ های اندوفیت از نظر تولید آنزیم ­های مختلف هیدرولازی بررسی شدند در این میان آنزیم پروتئاز در طیف وسیعی از باکتری­ های اندوفیت و به مقدار بیشتر از سایر آنزیم­ ها تولید شد. سویه EndoA، مورد شناسایی مولکولی قرار گرفت و مشخص شد که به میزان 100 درصد مشابه Bacillus halotolerans است.




کلیدواژه‌ها

آنزیم­ های هیدرولازی
باسیلوس هالوتولرانس
پروتئاز
زایلاناز
کیتیناز

عنوان مقاله English

Screening methods and enzyme profile of agricultural plant-endophytic bacteria

نویسندگان English

Samaneh Khosroshahi 1
Ensieh Salehghamari 2
Mohammad Ali Amoozegar 3
Parvaneh Saffarian 1
1 Department of Biology, Faculty of Basic Science, Islamic Azad University, Tehran, Iran
2 Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
3 Department of Microbiology, School of Biology, University College of Science, University of Tehran, Tehran, Iran
چکیده English

Nowadays plant endophytic bacteria have found diverse and useful applications in biotechnology; therefore, much attention has been paid to the isolation, identification, and evaluation of these microorganisms. Since the sterilizing plant tissue surfaces from epiphytic bacteria is difficulty, the efficacy of three different screening methods for endophytic bacteria including 1- HClO sterilization, 2- Periodic sterilization (modified tyndallization) and 3- Triton X100 and HClO sterilization, was evaluated in this study. The modified Tyndallization is an innovative method used in this study to appropriately remove the internal spores of epiphytic bacteria, considered to be an obstacle to the isolation of endophytes. Most of the endophytic bacteria were isolated from dicotyledons and leaves. Endophytic bacteria were also studied for the production of different hydrolase enzymes, whereas the protease enzyme was produced in a wide range of endophytic bacteria in greater quantities than other enzymes. The EndoA strain was molecularly identified and found to be 100% similar to Bacillus halotolerans.


کلیدواژه‌ها English

Bacillus halotolerans
chitinase
hydrolytic enzymes
protease
xylanas
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405

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