القاء پلی پلوئیدی در بابونه آلمانی با استفاده از علف کش تریفلورالین

نویسندگان

1 دانشگاه خلیج فارس

2 دانشگاه خوارزمی

چکیده
بابونه یک گیاه دارویی از تیرۀ کاسنیان است. این مطالعه به منظور بررسی اثر تریفلورالین بر القاء پلی­پلوئیدی در گیاه بابونه انجام شده­است. دو آزمایش مستقل به صورت فاکتوریل در قالب طرح بلوک­های کامل تصادفی بر روی دانه رست و جوانه­های رأسی در مرحله دو برگی انجام گرفت. به دنبال آنالیزهای ریخت­شناختی، بیوشیمیایی و سیتولوژیکی تعداد کروموزوم­ها برای انواع دیپلوئید و تتراپلوئید به ترتیب 18 و 36 مورد تائید قرار گرفت. بررسی­های ریخت­شناختی و بیوشیمیایی آشکار کرد که افزایش در تعداد مجموعه کامل کروموزومی با کاهش تعداد روزنه­ها در واحد سطح و افزایش اندازه روزنه، تعداد کلروپلاست و محتوای کلروفیل همراه است. همچنین القاء افزایش سطح پلی­پلوئیدی موجب کاهش ارتفاع گیاه و افزایش تعداد شاخه­های جانبی، اندازه برگ و قطر ساقه ، گل و نهنج شد. می­توان نتیجه گرفت که در هر دو روش، غلظت 5/22 میکرومولار تریفلورالین یک غلظت بهینه برای تولید بابونه تتراپلوئید با بالاترین میزان القاء پلی­پلوئیدی و پایین­ترین درصد ناهنجاری است.

کلیدواژه‌ها


عنوان مقاله English

Polyploidy Induction in German Chamomile (Matricaria chamomilla L.) by Herbicide Trifluralin

نویسندگان English

Leila Karami 1
Mohammad Modarresi 1
Mohammad Amin Kohanmoo 1
Fatemeh Zahabi Ahmadi 1
Saeed Irian 2
1 Faculty of Agriculture and Natural Resources
2 Faculty of Biological Sciences
چکیده English

Polyploidy induction in German chamomile (Matricaria chamomilla L.) by herbicide trifluralin



Leila Karami1, Mohammad Modarresi2, Mohammad Amin Kohanmoo2, Fatemeh Zahabi Ahmadi2 & Saeed Irian3

1Department of Horticulture, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, 7516913817, Iran; 2Department of Plant Breeding, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, 7516913817, Iran; 3Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.

Correspondent author: Leila Karami, leila.karami@pgu.ac.ir



Abstract. Chamomile (Matricaria chamomilla L.) is a medicinal herb belonging to Asteraceae family. This study was conducted to investigate the effect of trifluralin on the induction of polyploidy in chamomile. Two independent full factorial experiments on seedlings and 2-leaf stage apical buds were performed. Following morphological, biochemical and cytogenetic analysis, chromosome numbers of 18 and 36 were detected for diploid and tetraploid types, respectively. Morphological and biochemical examinations revealed that an increase in the number of full chromosome set results in a reduction in stomata number per unit area as well as an increase in stomata size, chloroplast number, and chlorophyll content. Induction of ploidy level increments also reduced plant height and increased the number of lateral branches, leaf size, and diameters of stems, flowers and receptacles. It is concluded that a concentration of 22.5 µM trifluralin in both methods is optimum for the production of tetraploid chamomile with the highest rate of polyploidy induction and the lowest percentage of abnormality.


کلیدواژه‌ها English

chromosome
Concentration
cytological changes
morphological traits
Time
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405