القای تغییرات ژنتیکی و مورفولوژی در کاتارانتوس روزئوس توسط پلاسمای اتمسفری سرد

نویسندگان

1 واحد علوم و تحقیقات، دانشگاه آزاد اسلامی

2 انستیتو پاستور ایران

3 دانشگاه آزاد اسلامی واحد قم

چکیده
گیاه کاتارانتوس روزئوس به طور گسترده ­ای در جهان کشت می­ شود. پلاسمای سرد اتمسفری به منظور بهبود جوانه زنی بذر و رشد گیاهان زراعی استفاده می شود. در تحقیق حاضرسه زمان مختلف پلاسمای اتمسفری سرد(40،50و60 ثانیه) به منظور تیمار بذرها استفاده و تغییرات در صفات مورفولوژی، آنزیم های آنتی اکسیدانی و محتوای ژنتیکی در این گیاهان بررسی شد. پلاسمای سرد (50 ثانیه) به طور قابل توجهی جوانه زنی، طول برگ ­ها و طول ساقه گیاهان را نسبت به گیاهان کنترل افزایش داد. فعالیت آنزیم های کاتالاز و پراکسیداز بالاترین میزان را در گیاهان تیمارشده با پلاسمای سرد به ترتیب در 40 و 60 ثانیه نشان دادند. نشانگر پلی مورفیسم تکثیریافته مرتبط با توالی (SRAP) بالاترین درجه تنوع ژنتیکی در گیاهان تیمارشده 50 ثانیه پلاسمای سرد نشان داد (Ne = 1.388, I = 0.316, He = 0.217, uHe = 0.237 and P % = 50.08). بر اساس روش نزدیکترین خویشاوندی (NJ)، آنالیز مولفه های اصلی (PCoA) و آنالیز واریانس مولکولی (AMOVA) ، چهار گروه مجزا شکل گرفت. گیاهان تیمار شده با 40 و 50 ثانیه پلاسمای سرد به علت تفاوت ژنتیکی با فاصله از گیاهان گروه کنترل قرار گرفتند. نتایج نشان می دهد پلاسمای اتمسفری سرد می تواند به عنوان ابزاری ایمن از لحاظ اقتصادی و زیست محیطی در افزایش رشد C. roseus و القای تغییرات ژنتیکی استفاده شود.

کلیدواژه‌ها


عنوان مقاله English

Induced genetic and morphological changes in Catharanthus roseus L. by cold atmospheric plasma

نویسندگان English

Zahra Noormohammadi 1
Mahnoosh Mohammadzadeh-Shahir 1
Donya Fahmi 1
Seyed Mohammad Atyabi 2
Farah Farahani 3
1 Islamic Azad University
2 Pasteur Institute
3 Islamic Azad University
چکیده English

Catharanthus roseus is widely cultivated around the world. Cold atmosphere plasma (CAP) has been used to improve crop plants seed germination, and plant growth. In the present study, three different times of CAP (40, 50 and 60s) were used for the treatment of C. roseus seeds, and the changes in morphological traits, antioxidant enzymes and the genetic content of the treated plants were examined. Cold plasma (50s) markedly raised the seed germination, length of the leaves and the stem length of the plants in comparison with those in the control group. The catalase and peroxidase enzyme activities had the highest value in 60 and 40 s CAP treated plants, respectively. The sequence related amplified polymorphism (SRAP) markers showed the highest degree of genetic diversity in 50s cold plasma treated plants (Ne = 1.388, I = 0.316, He = 0.217, uHe = 0.237 and P % = 50.08). Based on Neighbor Joining, principle coordination analysis (PCoA) and analysis of molecular variance (AMOVA) test, four significantly distant groups were formed. The 40s and 50s cold plasma treated plants stand far from the control plants due to genetic difference. The results indicate that cold atmosphere plasma could be used as an economic and environmentally safe tool in increasing C. roseus growth characteristics in addition to inducing genetic variations.




کلیدواژه‌ها English

Catalase
genetic diversity
Peroxidase
rose periwinkle
SRAP
Alves Junior, C., de Oliveira Vitoriano, J., Layza Souza da Silva, D., de Lima Farias M. and Bandeira de Lima Dantas, N. 2016. Water uptake mechanism and germination of Erythrina velutina seeds treated with atmospheric Plasma. ‒ Sci. Rep. 6: 33722 DOI: 10.1038/srep33722.
Apel, K. and Hirt, H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. ‒ Annu. Rev. Plant Biol. 55: 373-399.
Božena, Š., Špatenka, P., Šerý, M., Vrchotová, N. and Hrušková, I. 2010. Influence of plasma treatment on wheat and oat germination and early growth. IEEE Trans. ‒ Plasma Sci. 38: 2963-2968.
Caverzan, A., Passaia, G., Rosa, S.B., Ribeiro, C.W., Lazzarotto, F. and Margis-Pinheiro, M. 2012. Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection. ‒ Genet. Mol. Biol. 35: 1011-1019.
Chan S. W., Henderson I.R. & Jacobsen S.E. 2005. Gardening the genome: DNA methylation in Arabidopsis thaliana. – Nat. Rev. Genet. 6: 351-360.
Fatima, S., Mujib, A. and Tonk, D. 2015. NaCl amendment improves vinblastine and vincristine synthesis in Catharanthus roseus: a case of stress signalling as evidenced by antioxidant enzymes activities. – Plant Cell Tiss. Organ Cult. 121: 445-458.
Gendrel, A.V., Black, M., Vaughn, M.W., Dedhia, N., McCombie, W.R., Lavine, K., Mittal, V., May, B., Kasschau, K.D., Carrington, J.C., Doerge, R.W., Colot, V. and Martienssen, R. 2004. Role of transposable elements in heterochromatin and epigenetic control. ‒ Nature 430: 471-476.
Henselová, M., Slováková, L., Martinka, M. and Zahoranová, A. 2012. Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma. ‒ Biologia 67: 490-497.
Hoffmann, C., Berganza, C. and Zhang, J. 2013. Cold Atmospheric Plasma: methods of production and application in dentistry and oncology. ‒ Med. Gas Res. 3: 21
Jiafeng, J., Xin, H., Ling, L., Jiangang, L., Hanliang, Sh, Qilai, X., Renhong, Y. and Yuanhua, D. 2014. Effect of cold plasma treatment on seed germination and growth of wheat. ‒ Plasma Sci. Tech. 16: 54-58.
Koul, M., Lakra, N.S., Chandra, R. and Chandra, S. 2013. Catharanthus roseus and prospects of its endophytes: a new avenue for production of bioactive metabolites. ‒ Intl. J. Pharmaceut. Sci. Res. 4: 2705-2716.
Li, G. and Quiros, C.F. 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica. ‒ Theor. Appl. Genet. 103: 455-461.
Li, S.W. and Xue, L. 2010. The interaction between H 2 O 2 and NO, Ca 2+, cGMP, and MAPKs during adventitious rooting in mung bean seedlings. ‒In Vitro Cell. Develop. Biol. Plant 46: 142-148.
Ling, L., Jiafeng, J., Jiangang, L., Minchong, Sh., Xin, H., Hanliang SH. and Yuanhua D. 2014. Effects of cold plasma treatment on seed germination and seedling growth of soybean. ‒ Sci. Rep. 4: 5859 Doi:10.1038/srep05859
Ling, L., Jiangang, L., Minchong, Sh, Jinfeng, H., Hanliang, Sh., Yuanhua, D. and Jiafeng, J. 2016. Improving seed germination and peanut yields by cold plasma treatment. ‒ Plasma Sci. Tech.18: 1027-1033.
Maeder, M.L. Angstman, J.F., Richardson, M.E., Linder, S.J., Cascio, V.M., Tsai, S.Q., Ho, Q.H., Sander, J.D., Reyon, D., Bernstein, B.E., Costello, J.F., Wilkinson, M.F. and Joung, J.K. 2013. Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. ‒ Nat. biotechnol. 31: 1137-1142.
Meiqiang, Y., Mingjing, H. and Tengcai, M. 2005. Stimulating Effects of seed treatment by magnetized plasma on tomato growth and yield. ‒ Plasma Sci. Tech. 7: 3143-3147.
Meng, Y., Qu, G., Wang, T., Sun, Q., Liang, D. and Hu, Sh. 2017.Enhancement of germination and seedling growth of wheat seed using dielectric barrier discharge plasma with various gas sources. ‒ Plasma Chem. Plasma Process. 37: 1105-1119
Robarts, D.W.H. and Wolfe, A.D. 2014.Sequence-related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology. ‒ Appl. Plant Sci. 2: 1-13.
Sivachandiran, L. & Khacef, A. 2017. Enhanced seed germination and plant growth by atmospheric pressure cold air plasma: combined effect of seed and water treatment. ‒ RSC Adv. 7: 1822-1832.
Souza, A.D., Garc´ıa, D., Sueiro, L. and Gilart F. 2014. Improvement of the seed germination, growth and yield of onion plants by extremely low frequency non-uniform magnetic fields. ‒ Sci. Hort. 176: 63-69.
Swanberg, A. and Dai, W. 2008. Plant Regeneration of Periwinkle (Catharanthus roseus) via Organogenesis. ‒ Hortsci. 43: 832-836.
Uthup, T.K., Ravindran, M., Bini K. and Thakurdas, S. 2011. Divergent DNA methylation patterns associated with abiotic stress in Hevea brasiliensis. ‒ Mol. Plant 4: 996-1013.
Verma, A.K., Singh, R.R. and Singh, S. 2012. Improved alkaloid content in callus culture of Catharanthus roseus. ‒ Bot. Serb. 36: 123-130.
Yin, M.Q., Huang, M.J., Ma, B.Z. and Ma, T.C. 2005. Stimulating effects of seed treatment by magnetized plasma on tomato growth and yield. ‒ Plasma Sci. Tech. 7: 3143-3147.
Zargar, M., Farahani, F. and Nabavi, T. 2010. Hairy roots production of transgenic Catharanthus roseus L. plants with Agrobacterium rhizogenes under in vitro conditions. ‒J. Med. Plants Res. 4: 2199-2203.
Zhang, J.J., Oh Jo, J., Huynh, D.L., Kumar, Mongre, R., Ghosh, M., Singh, A., Lee, S.B., Sun Mok, Y., Hyuk, P. and Jeong, D.K. 2017. Growth-inducing effects of argon plasma on soybean sprouts via the regulation of demethylation levels of energy metabolism-related genes. ‒ Sci. Rep. 7: 41917 DOI: 10.1038/srep41917
Zhou, R., Zhou, R., Zhang, X., Zhuang, J., Yang, S., Bazaka, K. and Ostrikov, K. 2016. Effects of Atmospheric-Pressure N2, He, Air, and O2 Microplasmas on Mung Bean Seed Germination and Seedling Growth. ‒ Sci. Rep. 6: 32603 DOI: 10.1038/srep32603.

  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405