The effect of climate on water content, dormancy and dehydrins expression pattern in pistachio seeds

Zamani Bahramabadi, Elaheh; Jonoubi, Parisa; Rezanejad, Farkhondeh

doi:10.29252/nbr.6.1.124

The effect of climate on water content, dormancy and dehydrins expression pattern in pistachio seeds

Authors

Elaheh Zamani Bahramabadi ¹

Parisa Jonoubi ¹

Farkhondeh Rezanejad ²

¹ Kharazmi university of Tehran

² Shahid Bahonar university of Kerman

https://doi.org/10.29252/nbr.6.1.124

Abstract

The environmental conditions which the parent plant has undergone during seed development can affect many properties of the seeds such as degree of dormancy, weight and the amount of their compounds. In this study, mature pistachio seeds of Ahmadaghaei cultivar, collected from the two cities of Rafsanjan and Shahrbabak located in Kerman Province (Iran), were compared. It was found that the seeds collected from Shahrbabak being a cooler and wetter region had a longer dormancy, although the water content of the two seed series were similar. Dehydrin proteins generally play a role in protecting plant cells against dehydration stress. Dehydrin contents of the cotyledons and embryonic axes of the two seed series were compared by western blot method using an antibody against the conserved K segment in dehydrin proteins. It was found that the cotyledons had five dehydrin versions with molecular weights of 23, 25, 32, 39 and 48 kDa, the expression of which not being influenced by environmental factors. It was found that the embryonic axes had seven other versions of dehydrin with weights of 17, 19, 20, 28, 67, 77 and 98 kDa in addition to those five versions. This shows higher protection of embryonic axes compared with the cotyledons. The 25 and 28 kDa versions had higher expression levels in embryonic axes of Shahrbabak seeds, while the 39 kDa version had a higher expression level in embryonic axes of Rafsanjan seeds.

Keywords

cotyledon

Development

embryonic axis

Environment

western blotting

20.1001.1.24236330.1398.6.1.12.7

Baskin, C.C. and Baskin, J.M. 1998. Seeds - ecology, biogeography, and evolution of dormancy and germination. San Diego, CA, USA: Academic Press.

Banerjee, A and Roychoudhury, A. 2016. Group II late embryogenesis abundant (LEA) proteins: structural and functional aspects in plant abiotic stress. - Plant Growth. Regul. 79: 1-17. [DOI:10.1007/s10725-015-0113-3]

Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254. [DOI:10.1006/abio.1976.9999]

Bremer, A., Wolff, M., Thalhammer, A. and Hincha, D.K. 2017. Folding of intrinsically disordered plant LEA proteins is driven by glycerol-induced crowding and the presence of membranes. - FEBS J. 284: 919-936. [DOI:10.1111/febs.14023]

Carjuzaa, P., Castellión, M., Distéfano, A.J., del Vas, M. and Maldonado, S. 2008. Detection and subcellular localization of dehydrin-like proteins in quinoa (Chenopodium quinoa Willd.) embryos. - Protoplasma 233: 149-156. [DOI:10.1007/s00709-008-0300-4]

Close, T. 1997. Dehydrins: A commonalty in the response of plants to dehydration and low temperature. - Physiol. Plant. 100: 291-296. [DOI:10.1034/j.1399-3054.1997.1000210.x]

Daws, MI., Lydall, E., Chmielarz, P., Leprince, O., Mathews, S., Thanos, C.A. and Pritchard, H.W. 2004. Developmental heat sum inﬂuences recalcitrant seed traits in Aesculus hippocastanum across Europe. - New Phytol. 162: 157-166. [DOI:10.1111/j.1469-8137.2004.01012.x]

Fenner, M. 1991. The effects of the parent environment on seed germinability. - Seed 1: 75-84. [DOI:10.1017/S0960258500000696]

Fenner, M. 1992. Environmental influences on seed size and composition. - Hortic. Rev. 13: 183-213. [DOI:10.1002/9780470650509.ch5]

Figueroa, R., Herms, D.A., Cardina, J. and Doohan, D. 2010. Maternal environment effects on common groundsel (Senecio vulgaris) seed dormancy. - Weed Sci. 58: 160-166. [DOI:10.1614/WS-D-09-00006.1]

Gutterman, Y. 2000. Maternal effects on seeds during development. Pages 59-84 in: M. Fenner, (ed.). Seeds: The Ecology of Regeneration in Plant Communities. Wallingford, UK: CAB International. [DOI:10.1079/9780851994321.0059]

Jaganathan, G.K. 2016. Influence of maternal environment in developing different levels of physical dormancy and its ecological significance. - Plant Ecology. 217: 71-79. [DOI:10.1007/s11258-015-0560-y]

Kalemba, E.M. and Pukacka, S. 2008. Changes in late embryogenesis abundant proteins and a small heat shock protein during storage of beech (Fagus sylvatica L.) seeds. - Environ. Exper. Bot. 63: 274-280. [DOI:10.1016/j.envexpbot.2007.12.011]

Kalemba, E.M. and Pukacka, S. 2012. Association of protective proteins with dehydration and desiccation of orthodox and recalcitrant category seeds of three Acer genus species. - J. Plant Growth. Regul. 31: 351-362. [DOI:10.1007/s00344-011-9246-4]

Kermode, A.R. 1997. Approaches to elucidate the basis of desiccation-tolerance in seeds. - Seed Sci. Res. 7: 75-95. [DOI:10.1017/S0960258500003421]

Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature 227: 680-685. [DOI:10.1038/227680a0]

Mukherjee, K., Roychoudhury. A., Gupta. B., Gupta. S. and Sengupta, D.N. 2006. An ABRE binding factor, OSBZ8, is highly expressed in salt tolerant cultivars than in salt sensitive cultivars of indica rice. - BMC Plant Biol. 6: 1-14.

Panza, V., Distéfano, A.J., Carjuzaa, P., Láinez, V., Del Vas, M. and Maldonado, S. 2007. Detection of dehydrin-like proteins in embryos and endosperm of mature Euterpe edulis seeds. - Protoplasma 231: 1-5. [DOI:10.1007/s00709-007-0248-9]

Saber Amoli, S., Naseri, A., Rahmani, G.H. and Kalirad, A. 2004. Medicinal Plants in Kerman Province. - Iranian Medicinal and Aromatic Plants Research 20: 487-532.

Steadman, K.J., Ellery, A.J., Chapman, R., Moore, A. and Turner, N.C. 2004. Maturation temperature and rainfall influence seed dormancy characteristics of annual ryegrass (Lolium rigidum). - Aust. J. Agric. Res. 55: 1047-1057. [DOI:10.1071/AR04083]

Takahashi, R., Joshee, N. and Kitagawa, Y. 1994. Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice. - Plant Mol. Biol. 26(1): 339-352. [DOI:10.1007/BF00039544]

Tollenaar, M. 1999. Duration of the grain-filling period in maize is not affected by photoperiod and incident PPFD during vegetative phase. - Field Crops Res. 62: 15-21. [DOI:10.1016/S0378-4290(98)00170-1]

Vaseva, I.I, Anders, I. and Feller, U. 2014. Identification and expression of different dehydrin subclasses involved in the drought response of Trifolium repens. - J. Plant. Physiol. 171: 213-224. [DOI:10.1016/j.jplph.2013.07.013]

Wang, Y., Xu, H., Zhu, H., Tao, Y., Zhang, G., Zhang, L., Zhang, C., Zhang, Z. and Ma. Z. 2014. Classification and expression diversification of wheat dehydrin genes. - Plant Sci. 214: 113-120. [DOI:10.1016/j.plantsci.2013.10.005]

Zamani Bahramabadi, E., Jonoubi, P. and Rezanejad, F. 2018. Ultrastructural changes of pistachio (Pistacia vera L.) mature seeds and pollen in relation to desiccation. - Trees 32: 29-39. [DOI:10.1007/s00468-017-1606-7]