سیستم های بیان پروتئین مبتنی بر گیاه برای تولید پروتئین و پپتید ضدمیکروبی

نویسنده

دانشگاه خوارزمی

چکیده
گیاهان یک سیستم بیانی برای تولید پروتئین ­های هترولوگ، به خصوص پروتئین­ های نوترکیب درمانی هستند. سال­ های اخیر، اکثر پروتئین­ های نوترکیب در باکتری­ ها یا سلول­های پستاندار تولید می­شوند. اگر چه گاهی سلول­ های حشرات، مخمر و قارچ برای تولید پروتئین­ های نوترکیب استفاده می ­شود. محصول سیستم گیاهی می­تواند سالم، اقتصادی و راحت باشد و این امکان وجود دارد به طور گسترده در کشاورزی و صنایع، به ویژه در علوم زیستی و صنعت داروسازی استفاده شود. گیاهان سیستم­های بیانی پروتئینی مناسب در تولید با کیفیت، هزینه کم و تغییرات پساترجمه­ای صحیح هستند. استفاده از گیاه تراریخته در تولید واکسن­ ها، آنتی­بادی­ها و پروتئین­های دارویی در سال­های اخیر تبدیل به یک نقطه عطف در مهندسی ژنتیک گیاهی شده است. استراتژی­های کارآمد برای تولید پروتئین­ های نوترکیب اهمیت بیشتری را به دست می ­آورند، به عنوان برنامه­ های کاربردی و مهم نیاز به مقدار زیادی از پروتئین ­های نوترکیب با کیفیت بالا برای استفاده در صنعت است. در این مقاله، مشکلات رایج در تولید پروتئین­ های نوترکیب و پپتید ضدمیکروبی در سیستم ­های بیانی پروتئین مبتنی بر گیاه مورد بررسی قرار گرفته و استراتژی­ های راه حل آنها مورد بحث قرار گرفته است.

کلیدواژه‌ها


عنوان مقاله English

Plant-based expression systems for protein and antimicrobial peptide production

نویسنده English

Azadeh Niknejad
Department of Cell and Molecular Sciences, School of Biological Science, Kharazmi University, Tehran, IRAN
چکیده English

Molecular farming technology offers a unique advantage that almost any protein can be produced economically and safely under very controlled conditions. Besides traditional production systems, such as bacteria, yeasts, insects and mammal cell lines, plants can now be used to produce eukaryotic recombinant proteins, especially therapeutic ones. Their advantages as hosts for protein production include correct post-translational modifications, low-cost maintenance and no risk of contamination by human pathogens. The system is widely applied in agriculture and industry, especially in life science and pharmaceutical industry. The application of transgenic plants in the production of vaccines, antibodies and pharmaceutical proteins has been playing a key role in plant genetic engineering in recent years. The production of recombinant proteins plays a critical role in the production of high amounts of high-quality proteins. In this review, common problems in the production of recombinant proteins and antimicrobial peptides in plant-based expression systems are discussed and strategies for their solution are suggested. Viral vector-mediated transient gene expression in plants enables rapid production of pharmaceutical proteins such as vaccine antigens and antibodies. To conclude, plant-based systems have the potential to bring unique efficacy-enhancing features to increase the utility and effectiveness of vaccines and therapeutics.

کلیدواژه‌ها English

antibodies
pharmaceutical proteins
therapeutic proteins
transgenic plant
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  • تاریخ انتشار 18 خرداد 1405