Nova Biologica Reperta

Investigation of hairy root induction in some Salvia L. species

Authors

Reza Norouzi1 1
Mesbah Babalar 2
Masoud Mirmasoumi3 3

1 University of Mohaghegh Ardabili

2 2University College of Agriculture and Natural Resources, University of Tehran

3 University of Tehran

https://doi.org/10.21859/acadpub.nbr.4.2.173
Abstract
Hairy root induction in plants is the result of the insertion of T-DNA from Agrobacterium rhizogenes into the plant genome. The present study was conducted to investigate the effect of bacterium strain and plant species type on hairy root induction in two endemic (Salvia eremophila and S. reuterana) and five non-endemic (S. macrosiphon, S. multicaulis, S. nemorosa, S. verticellata and S. virigata) Salvia by four bacteria strains including 1724, 2659, ATCC-15834 and A4. Petiole and stem explants were not capable of inducing hairy roots, while almost all leaf segments produced it. Confirmatory studies were carried out by direct detection of inserted rol C by the PCR. The results showed that different Agrobacterium rhizogenes strain and Salvia spices had significant effect on hairy roots number and frequency. The infection of S. macrosiphon via A. rhizogenes strain ATCC15834 showed the highest number of infe-cted roots per explant (5.12 hairy roots) and root frequency (82%). The highest number of hairy root per explant in S. eremophila (3.32 hairy roots) and S. reuterana (3.92 hairy roots) were achieved by inoculation with strain A4. Strain 2659 produced the highest hairy roots number in S. nemorosa (2.6 hairy roots), S. multicaulis (4.36 hairy roots) and S. verticillata (5 hairy roots). Also hairy roots formation occurred at the highest number in S. virigata (3 hairy roots) with infection by strain ATCC15834.

Keywords

Agrobacterium rhizogenes
transformed root
leaf explant
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Nova Biologica Reperta
Volume 4, Issue 2 - Serial Number 12
Summer 2017
Pages 173-180

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026

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