Histological investigation of the effects of nano titanium dioxide on the development of the first mandibular molar bud in NMRI mouse strain in vivo

Tabatabaei Nia, Seyedeh Sadaneh; Farhadi, Mona; Parivar, Kazem

doi:10.21859/acadpub.nbr.4.2.137

Histological investigation of the effects of nano titanium dioxide on the development of the first mandibular molar bud in NMRI mouse strain in vivo

Authors

Seyedeh Sadaneh Tabatabaei Nia ¹

Mona Farhadi ²

Kazem Parivar ¹

¹ Tehran Science and Research Branch, Islamic Azad University

² Islamic Azad University of Karaj

https://doi.org/10.21859/acadpub.nbr.4.2.137

Abstract

Nowadays, with the applications of titanium dioxide nanoparticles (TiO₂-NPs) in pharmacy, food industry, cosmetics, toothpaste and sunscreens, pregnant women are exposed to nanoparticles. Since tooth development is vuln-erable to environmental impacts and mandibular first molar bud develops before maxillary first molar bud, in this ex-perimental study the effects of TiO₂-NPs on the development of first mandibular molar bud in NMRI mouse was inve-stigated. Twenty five female NMRI mice were randomly divided into five groups (N=5); Control group (pregnant mice without any treatment), sham group (treated with distilled water), experimental groups 1, 2 and 3 (treated with 50, 150 and 500 mg/kg BW TiO₂-NPs, respectively, via gavage from embryonic days 10.5-14.5). On E14.5, embryos heads were prepared for histological examination and dental tissues were evaluated. Data were analyzed by ANOVA and post hoc test (Tukey). Microscopic observation showed tissue disorganization in experimental groups. Findings showed that in experimental groups 1 and 2, the diameter of bud and dental papilla and the length of dental bud decreased sign-ificantly. In experimental group 2, decrease in the diameter of dental follicle, dental bud and dental papilla and the le-ngth of dental bud was significant. On the other hand, in experimental group 3, only the decrease in the length of dental bud was significant. These findings showed that nano titanium dioxide can reduce the size of dental buds and is capable of preventing tooth development.

Keywords

Histology

odontogenesis

bud stag

cap stage

TiO2

gavage

20.1001.1.24236330.1396.4.2.9.2

Buzea, C., Pacheco, I.I., and Robbie, K. 2007. Nanomaterials and nanoparticles: sources and toxicity. – Biointerphases 2: 17-71.
Chen, T., Yan, J., and Li, Y. 2014. Genotoxicity of titanium dioxide nanoparticles. – J. Food Drug. Anal. 22: 95-104.
Cohn, S.A. 1957. Development of the molar teeth in the albino mouse. – Am. J. Anat. 101: 295-319.
Dechici, P., and Moura, C.C.G. 2005. First molar deve-lopment in Calomys callosus. – Biosci. J. 21: 159-166.
Dehghani, N., Noori, A., and Modaresi, M. 2014. Investigating the effect of titanium dioxide nano-particles on the growth and sexual maturation of male rats. – Int. J. Basic Sci. Appl. Res. 3: 772-776.
Duan, Y., Liu, J., Ma, L., Li, N., Liu, H., Wang, J., Zheng, L., Liu, C., Wang, X., Zhao, X., Yan, J., Wang, S., Wang, H., Zhang, X., and Hong, F. 2010. Toxi-cological characteristics of nanoparticulate anatase titanium dioxide in mice. – Biomaterials 31: 894-899.
Fujishima, A., Rao, T.N., and Tryk, D.A. 2000. Titanium dioxide photocatalysis. – J. Photochem. Photobiol. C Photochem. Rev. 1: 1-21.
Gui, S., Sang, X., Zheng, L., Ze, Y., Zhao, X., Sheng, L., Sun, Q., Cheng, Z., Cheng, J., Hu, R., Wang, L., Hong, F., and Tang, M. 2013. Intragastric exposure to titanium dioxide nanoparticles induced nephrotoxicity in mice, assessed by physiological and gene expression modifications. – Part. Fibre Toxicol. 10: 4. doi: 10.1186/1743-8977-10-4.
Guo, L., Liu, X., Qin, D., Gao, L., Zhang, H., Liu, J., and Cui, Y. 2009. Effects of nanosized titanium dioxide on the reproductive system of male mice. – Zhonghua Nan Ke Xue 15: 517-522.
Hayati Roodbari, N., Parivar, K., Rezaee, S., and Badiee, A. 2014. Comparison of the effects of nano titanium dioxide with 10nm diameter on testis and E-pididy-mis of edult male mice NMRI strain in “in vivo” conditions. – EAB J. 3: 27 38.
Hayati Roodbari, N., Parivar, K., Badiei, A., and Zolfaghari Barogh, S. 2014. Cytotoxic effects of nano-titanium dioxide on forelimb bud development in NMRI mouse embryos in vivo. – ZUMS J. 22: 11-24.
Heikkinen, T., Alvesalo, L., and Osborne, R.H. 1994. Intercuspal distances of the first permanent molar with special reference to maternal smoking during pregnancy. – Acta Med. Auxol. 26: 103-114.
Heikkinen, T., Alvesalo, L., Osborne, R.H., Pirttiniemi, P., and Tienari, J. 1992. Maternal smoking and tooth formation in the foetus. I. Tooth crown size in the deciduous dentition. – Early Hum. Dev. 40: 49-59.
Heikkinen, T., Alvesalo, L., Osborne, R.H., and Tienari, J. 1994. Maternal smoking and tooth formation in the foetus. II. Tooth crown size in the permanent dentiti-on. – Early Hum. Dev. 40: 73-86.
Hiatt, J.L., Gartner, L.P., and Provenza, D.V. 1974. Molar development in the mongolian gerbil (merion-es unguiculatus). – Am. J. Anat. 141: 1-21.
Löschner, K., Larsen, E.H., Birkedal, R.K., Vibenholt, A., Boisen, A.M.Z., Wallin, H., and Vogel, U. 2010. Effects of prenatal exposure to surface-coated nano-sized titanium dioxide (UV-Titan). A study in mice. – Part. Fibre Toxicol. 7: 1-15.
Iavicoli, I., Leso, V., Fontana, L., and Bergamaschi, A. 2011. Toxicological effects of titanium dioxide nano-particles: a review of in vitro mammalian studies. – Eur. Rev. Med. Pharmacol. Sci. 15: 481-508.
Jeon, Y.M., Kim, W.J., and Lee, M.Y. 2012. Studies on liver damage induced by nanosized-titanium dioxide in mouse. – J. Environ. Biol. 34: 283-287.
Kang, S.J., Kim, B.M., Lee, Y.J., and Chung, H.W. 2008. Titanium dioxide nanoparticles trigger p53-mediated damage response in peripheral blood lymphocytes. – Environ. Mol. Mutagen. 49: 399-405.
Kim, J.Y., Cha, Y.G., Cho, S.W., Kim, E.J., Lee, M.J., Lee, J.M., Cai, J., Ohshima, H., and Jung, H.S. 2006. Inhibition of apoptosis in early tooth development alters tooth shape and size. – J. Dent. Res. 85: 530-535.
Kiukkonen, A. 2006. Toxicity of dioxin to developing teeth and salivary glands. – Dep. Oral Pathol. Citeseer, Helsinki.
Lefkowitz, W., Bodecker, C.F., and Mardfin, D.F. 1953. Odontogenesis of the rat molar: prenatal stage. – J. Dent. Res. 32: 749-772.
McCormick, J. 2006. Chemistry of TiO2 Nanoparticles. - Ph.D. Thesis, Fac. Univ. Delaware. University of Delaware.
Meena, R., Kumar, S., and Paulraj, R. 2015. Titanium oxide (TiO2) nanoparticles in induction of apoptosis and inflammatory response in brain. – J. Nanoparticle Res. 17: 1-14.
Meena, R., Rani, M., Pal, R., and Rajamani, P. 2012. Nano-TiO2-induced apoptosis by oxidative stress-mediated DNA damage and activation of p53 in human embryonic kidney cells. – Appl. Biochem. Biotechnol. 167: 791-808.
Oberdörster, G., Maynard, A., Donaldson, K., Castranova, V., Fitzpatrick, J., Ausman, K., Carter, J., Karn, B., Kreyling, W., Lai, D., Olin, S., Monteiro-Riviere, N., Warheit, D., and Yang, H. 2005. Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. – Part. Fibre Toxicol. 2: 8. doi.org/10.1186/1743-8977-2-8.
Oberdörster, G., Oberdörster, E., and Oberdörster, J. 2005. Nanotoxicology: an emerging discipline evol-ving from studies of ultrafine particles. – Environ. Health Perspect. 113: 823-839.
Payne, T.M., Gartner, L.P., Hiatt, J.L., and Provenza, D.V. 1977. Molar odontogenesis in the hairless mouse. – Acta Anat. (Basel). 98: 264-274.
Russo, P.L., and Gartner, L.P. 1987. First molar odonto-genesis in the golden Syrian hamster (Cricetus auratus). – Anat. Anz. 164: 275-289.
Salmela, E. 2011. The effects of organic environmental toxicants on hard tissue formation in developing to-oth; an in vitro study in mice. – Ph.D thesis, Unive-rsity of Helsinki.
Sayes, C.M., Wahi, R., Kurian, P.A., Liu, Y., West, J.L., Ausman, K.D., Warheit, D.B., and Colvin, V.L. 2006. Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. – Toxicol. Sci. 92: 174-185.
Shi, H., Magaye, R., Castranova, V., and Zhao, J. 2013. Titanium dioxide nanoparticles: a review of current toxicological data. – Part. Fibre Toxicol. 10: 1-33.
Umezawa, M., Tainaka, H., Kawashima, N., Shimizu, M., and Takeda, K. 2012. Effect of fetal exposure to titanium dioxide nanoparticle on brain development & minus; brain region information. – J. Toxicol. Sci. 37: 1247-1252.
Wang, J., Zhou, G., Chen, C., Yu, H., Wang, T., Ma, Y., Jia, G., Gao, Y., Li, B., and Sun, J. 2007. Acute toxicity and biodistribution of different sized titanium dioxide particles in mice after oral administration. – Toxicol. Lett. 168: 176-185.
Xiao, X., Ouyang, K., Liu, R., and Liang, J. 2009. Anatase type titania nanotube arrays direct fabricated by anodization without annealing. – Appl. Surf. Sci. 225: 3659-3663.
Zhao, X., Ze, Y., Gao, G., Sang, X., Li, B., Gui, S., Sheng, L., Sun, Q., Cheng, J., Cheng, Z., Hu, R., Wang, L., and Hong, F. 2013. Nanosized TiO(2)-induced reproductive system dysfunction and its mechanism in female mice. – PLoS One 8: e59378.