Response of genes effective in apoptosis in the liver and intestinal cells of broiler chickens fed silver nanoparticles coated on clinoptilolite under heat stress condition

Hashemi, Seyed Reza; Akhondpor, Negin; Farhadi, Ayoub; Arabiyan, Elnaz

doi:10.61186/nbr.10.4.42

Response of genes effective in apoptosis in the liver and intestinal cells of broiler chickens fed silver nanoparticles coated on clinoptilolite under heat stress condition

Authors

Seyed Reza Hashemi ¹

Negin Akhondpor ¹

Ayoub Farhadi ²

Elnaz Arabiyan ¹

¹ , Gorgan University of Agricultural Sciences and Natural Resources

² Sari Agricultural Sciences and Natural Resources University

https://doi.org/10.61186/nbr.10.4.42

Abstract

The aim of this study was to investigate the effective genes on apoptosis (BAX and Bcl₂) in liver and intestinal cells of broiler chickens fed silver nanoparticles coated on clinoptilolite under acute heat stress induction. 450 d old broiler chicks (Cobb 500) were used in five treatments and six repetitions, and 15 pieces were used in each experimental unit in the form of a completely random design. Experimental diets were: 1) control or basal diet 2) basal diet supplemented by 1% clinoptilolite 3) basal diet supplemented by 1% clinoptilolite coated with 0.5% nanosilver 4) basal diet supplemented by 0.15% organic acid and 5) basal diet supplemented by 1% clinoptilolite coated with 0.5% nanosilver and 0.15% organic acid. Silver nanoparticles coated on clinoptilolite were investigated using XRF and FTIR techniques. In order to induce heat stress, the birds were affected by heat stress for one week in the last week of the breeding period, and on the last day of the stress, liver and intestine samples were obtained to check gene expression. The results of this experiment show that the treatments of clinoptilolite and silver nanoparticles coated on clinoptilolite have an increasing effect on the expression of Bcl₂ and Bax, while this effect was not seen in the organic acid treatment. In conclusion, it could be said that if silver nanoparticles are used in feeding livestock and poultry, it is better to use organic acid supplements to reduce the side effects of silver nanoparticles.

Keywords

Apoptosis

BAX

Bcl2

Silver nanoparticles

stress

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