The effect of silver nanoparticles on ovarian histopathological changes in mice treated with isoniazid

Samani Jahromi, Elahe; Rezai-Zadeh, Madjed; Zolghadri Jahromi, Samaneh

doi:10.21859/acadpub.nbr.3.4.354

The effect of silver nanoparticles on ovarian histopathological changes in mice treated with isoniazid

Authors

Elahe Samani Jahromi

Madjed Rezai-Zadeh

Samaneh Zolghadri Jahromi

https://doi.org/10.21859/acadpub.nbr.3.4.354

Abstract

The study attempts to evaluate the effects of silver nanoparticles on preventing the destruction of ovarian tissue in mice treated with isoniazid. In this experiment, 40 adult female rats were randomly divided into 5 groups (n=8) including: control group (no treatment), sham group (0.2 mg / kg normal saline), experimental group 1 (50mg / kg Ison-iazid), experimental group 2 (50mg / kg Isoniazid and 0.25mg/kg silver nanoparticles) and the experimental group 3 (50mg / kg isoniazid and 0.50mg / kg silver nanoparticles). All injections were prescribed for 15 days and Isoniazid and silver nano-particles were received orally and intraperitoneally, respectively. All the animals were operated on and their ovarian tissues were removed and placed in 10% formalin solution. Then H & E staining was performed for histological studies. In experimental groups 1, 2 and 3, reduction in the number of secondary follicles and corpus luteum was obse-rved in comparison with the control group. There was a significant increase in the number of primordial and graafian follicles in experimental group 3 when it was compared with isoniazid group. In addition, a significant increase was observed in the number of primary monolayer follicles in experimental groups 2 and 3 in comparison with the experi-mental group 1. It should be assumed that by the utilization of the minimum dose of the silver nanoparticles, its toxic effects on the ovarian tissue could be ignored. Therefore, the minimum dose of the silver nanoparticles can reduce the isoniazid effects on ovarian tissue.

Keywords

graf follicles

corpus luteum

primary monolayer follicles

Destruction

20.1001.1.24236330.1395.3.4.9.9

Ahamed, M., Alsalhi, M.S. and Siddiqui, M. 2010. Silver nanoparticle applications and human health. – Inter. J. Clinic. Chem. 411: 1841-1848.
Amirkhani-Dehkordi, Z., Naghsh, N., Nuri, A. and Agha-baba, H. 2013. Comparison of injective and contact ef-fect of silver nanoparticles on the rate of hemoglobin ch-anges in male rats. – Hakim Seyed Esmail Jorjani J. 14: 38-43.
Cai, Z., Barry, C.E. and Huner, Z.B. 2006. Mechanism of action of isoniazid. – Mol. Microb. 62: 1220-1227.
Christoforidis, G., Frank, J., Lindau, M., Lockman, G., Jones, C. and Petricoin, E. 2006. Nanoparticles: pote-ntial biomarker harvesters. – Curr. Opin. Chem. Biol. 10: 56-61.
Eom, H.J. and Choi, J. 2010. P 38 MAPK activation, DNA damage, cell cycle arrest and apoptosis as mechanisms of toxicity of silver nanoparticles in Jurkat T cells. – Envi-ron. Sci. Technol. 44: 8337-8342.
Fukuoka, A., Sakamoto, Y. and Guan, S. 2001. Novel te-mplating synthesis of necklace shaped mono and bi-metallic nanowires in hybrid organic inorganic me-soporous material. – Jam Chem. 123: 3373-3374.
Gajalakshmi, V., Peto, R., Kanaka, T.S. and Jha, P. 2003. Smoking and mortality from tuberculosis and other dise-ases in India: retrospective study of 43000 adult male de-aths and 35000 controls. – Lancet 362: 507-515.
Georgieva, N., Gadjeva, V. and Tolekova, A. 2004. New is-onicotinoylhydrazones with SSA protect against oxid-ative-hepatic injury of isoniazid. – TJS 2: 37-43.
Ghorbanzadeh, V., Moshtaghian, J. and Ebadi, A.H. 2011. Influence of nano-silver on graffian follicles via intr-aperitoneal injection in rats. – Middle-East J. Scien. Re-sea. 8: 228-230.
Gopinath, P., Gogoi, S.K., Sanpui, P., Paul, A., Chattopa-dhyay, A. and Ghosh, S.S. 2010. Signaling gene casc-ade in silver nanoparticle induced apoptosis. – Colloids and Surfaces B: Biointerfaces 77: 240-245.
Hooshiar, L. and Rezaei, S. 2010. The effect of silver na-noparticle on the ovarian tissue and hypophysis gonad axis on Wistar rat. – Thesis of MSC, PNU, Tehran. pp 1-20.
Huang, X., Choi, Y., Im, H. 2006. Aspartate aminotra-nsferase (AST/GOT) and alanine aminotransferase (ALT/GPT) detection techniques. – Sensors 6: 756-782.
Hussain, S., Hess, K., Gearhart, J., Geiss, K. and Schlager, J. 2005. In vitro toxicity of nanoparticles in BRL 3A rat liver cells. – Toxicol. In vitro 19: 975-983.
Khodadadi‚ S., Naghsh‚ N. and Mashayekh, A. 2013. Ef-fects of silver nanoparticle on phospho creatine kinase and histological changes of skeletal muscle tissue in male Wistar rat. – Mazand. Univ. Med. Sci. J. 23: 36-41.
Liu, G., Men, P., Harris, P.L., Rolston, R.K., Perry, G., and Smith, M.A. 2006. Nanoparticle iron chelators: a new therapeutic approach in Alzheimer disease and other neurologic disorders associated with trace meta-l imbalance. – Neurosci. Let. 406:189-193.

Long, T.C., Saleh, N., Tilton, R.D., Lowry, G.V. and Vero-nesi, B. 2006. Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity. – Env. Sci. Technol. 40: 4346-4352.
Malekigorji, M., Curtis, A.D.M. and Hoskims, C. 2014. The use of Iron oxide nanoparticles for pancreatic cancer therapy. – J. Nanomed. Res. 1: 0004. Dol: 10.15406/ jnmr.2014.01.0004.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. – Trends in Plant Sci. 7: 405-410.
Mohajeri, D., Doustar, Y. and Rahmani, J. 2011. Antiox-idant activity of green tea extract against Isoniazid in-duced hepatotoxicity in the rats. – Veterinary J. 5: 1221-1232.
Nel, A., Xia, T., Mädler, L. and Li, N. 2006. Toxic pote-ntial of materials at the nanolevel. – Sci. 311: 622-627.
Oberdörster, G., Stone, V. and Donaldson, K. 2007. Toxi-cology of nanoparticles: a historical perspective. – Nano-toxicol. 1: 2-25.
Ranjbar, A., Ataie, Z., Khajavi, F. and Ghasemi, H. 2013. Effects of silver nanoparticle on oxidative stress biom-arkers in rat. – Nanomed. J. 1: 205-211.
Razavian, M.H., Safarpour, E., Rohani, K., Yazdian, M.R. and Heidarian N. 2011. Study of some biochemical and hematological parameters changes of Wistar rats blood parallel to oral nanosilver consumption. – J. Babol Univ-ersity Med. Sci. 13: 22-27.
Rezaee Ranjbar Sardari, R., Rezaei Zarchi, S., Nasri, S., Talebi, A.R., Khoradmehr, A., Razavi Sheshde, A.R. and Adib, M. 2012. Toxicological effects of silver na-noparticles in rats lung. – Shahid Sadoughi Univ. Med. Sci. J. 20: 269- 276.
Stone, V. and Donaldson, K. 2006. Nanotoxicology: signs of stress. – Nature Nanotechnol. 1: 23-24.
Sukumaran, P. and Eldho, K.P. 2012. Silver nanopart-icles: mechanism of antimicrobial action, synthesis, me-dical applications, and toxicity effects. – Inter. Nano Lett.2:32 doi:10.1186/2228-5326-2-32.
Sun, Y., Oberley, L.W. and Li, Y. 1988. A simple method for clinical assay of superoxide dismutase. – Clinical Chem. 34: 497-500.
Tang, J. and Xi, T. 2008. Status of biological evaluation on silver nanoparticles. – Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 25: 958-961 (in Chinese).