Geometric analysis of the scale shape to discriminate different populations of Garra rufa Heckel, 1843

Authors

Abstract
Garra rufa is one of the 73 Garra species of the family Cyprinidae. Scales are used more commonly than any other structure in population dynamic studies, because of their transparency, ease of sampling, and the minimal in-juries caused to the fish during their removal. To discriminate fish from different rivers including Bashar, Behbahan, Gerdab, Kheirabad, Kooshk-e-Bahram, Mazoo, Palangan, Sendegan, Shoor-Abad, and Sirvan Rivers using geometric morphometric analyses of the scale shape, landmark based geometric morphometrics method was used. Seven landmark points were depicted on each scale using the software TpsDig2. After procrustes analyses, landmark data were exposed to principle component analysis (PCA), CVA, and cluster analyses using the past software. The results showed that th-ere were significant scale shape differences among some populations, but most of the samples showed overlapping sh-ape distribution and could not be separated robustly. Among the samples which were analyzed, only the Sendegan and Gerdab river samples did not show significant difference in scale shape compared to all other samples.

Keywords


Abdoli, A. 2000. The inland freshwater fishes of Iran. – Ir-anian Museum of Natural and Wildlife 378 pp, Tehran.
Akbarzadeh, A., Karami, M., Nezami, S.A., Mojazi, A.B., Khara, H. and Eagderi, S. 2009. A co-mparative study of morphometric and meristic characters of pikeperch Sa-nder lucioperca (L.) in Iranian waters of Caspian Sea and Aras Dam Lake. – J. Fish. (Iranian J. Nat. Resources) 22: 535-545.
Coad, B.W. 1995. Freshwater Fishes of Iran. – Acta Scientiarum Naturalium Academiae Scientiarum Bohe-micae 29: 1-64.
Coad, B.W. 2016. [Website] http://briancoad.com (acessed 3 March, 2016).
Coillie, V.R. and Rousseau, A. 1974. Composition minerate desecailles du Catostomus commersoni issued deux milieus differents: etude par microscopie electronique and analytique. – J. Fish. Res. Board. CAN. 31: 63-66.
Esmaeili, H.R., Ebrahimi, M., Ansari, T.H., Teimory, A., and Gholamhosseini, G. 2009. Karyotype analysis of Persian stone lapper, Garra persica Berg, 1913 (Acti-nopterygii: Cyprinidae) from Iran. – Curr. Sci. India. 96: 959-962.
Ghalenoei, M., Pazooki, J., Abdoli, A., Hassanzadeh Kiabi, B. and Golzarianpour, K. 2010. Morphometric and meristic study of Garra rufa populations in Tigris and Persian Gulf Basins. – Iran. Sci. J. Fisheries. 3: 107-118.
Grassberger, M., and Hoch, W. 2006. Ichthyotherapy as alternative treatment for patients with psoriasis: A pilot study. – Evid-Based Compl. Alt. 3: 483-488.
Hashemzadeh Segherloo, I., Abdoli, A., Eagderi, S., Esmaeili, H.R., Sayyadzadeh, G., Bernatchez, L., Hallerman, E., Geiger, M.F., Özulug, M., Laroche, J. and Freyhof, J. 2017. Dressing down: convergent reduction of the mental disc in Garra (Teleostei: Cypr-inidae) in the Middle East. – Hydrobiologia. 785: 47-59.
Ibanez, A. L., Cowx, I.G. and O'Higgins, P. 2007. Geometric morphometric analysis of fish scales for iden-tifying genera, species, and local populations within the Mugilidae. – Can. J. Fish. Aquat. Sci. 64: 1091-1100.




Jawad, L.A. and Al-Jufaili, S.M. 2007. Scale morphology of greater lizardfish Saurida tumbil (Bloch, 1795) (Pisc-es: Synodontidae). – J. Fish Biol. 70: 1185-1212.
Johal, M. S. and Dua, A. 1994. SEM study of the scales of freshwater snakehead, Chana nctatu (Bloch) upon expo-sure to endosulfan. – B. Environ. Contam. Tox. 52: 718-721.
Johal, M.S. and Sawhney, A.K. 1997. Lepidontal alterati-ons of the circuli on the scales of freshwater snakehead, Channa punctatus (Bloch) upon exposure to malathion. – Curr. Sci. 72: 367-369.
Kilambi, R.V and Zdinak, A. 1981. Comparison of early developmental stages and adults of Grass Carp Cten-opharyngodon idella, and hybridcarp (female grass Carp × male bighea, (Aristichthys nobilis). – J. Fish Biol. 19: 457-465.
Mathews W.J. 1988. Morphology, habitat use, and life history. In Patterns in Freshwater Fish Ecology. – Chapman & Hall, 756 pp, New York.
Pinheiro, A., Teixeira, C.M., Rego, A.L., Marques, J.F. and Cabral, H.N. 2005. Genetic and morphological variation of Solea lascaris (Risso, 1810) along the Portugese coast. – Fish. Res. 73: 67-78.
Poulet, N., Berrebi, P., Crivelli, A.J., Lek, S. and Argillier, C. 2004. Genetic and morphometric variation in the pikeperch (Sander lucioperca) of a fragmented delta. – Archive fur Hydrobiolgie. 159: 531-554.
Poulet, N., Reyjol, Y., Collier, H. and Lek, S. 2005. Does fish scale morphology allow the identification of popu-lations at a local scale? A case study for rostrum dace Leuciscus leuciscus burdigalensis in River Viaur (SW France). – Aquat. Sci. 67: 122–127.
Rohlf, F.J. 2003. tpssmal, thin-plate spline, version 1.20. – Department of Ecology and Evolution, State University of New York at Stony Brook.
Ruban, G.I. 1998. On the species structure of the Siberian sturgeon Acipenser baerii Brandt (Acipenseridae). – J. Ichthyol. 38: 345 – 365.
Sattari, M. 2003. Ichthyology I, anatomy and Physiology. – Guilan University, 680 pp.
Swain, D.P. and Foote, C.J. 1999. Stocks and chameleons: The use of phenotypic variation in stock identification. – Fish. Res. 43: 113-128.
Tabatabei, S.N., Eagderi, S., Hashemzadeh Segherloo, I. and Abdoli, A. 2014. Geometric and morphometric analysis of fish scales to identity genera, species and po-pulations case study: the Cyprinid family. – Taxo. Biosystem. 8:1-17.
Tudela, S. 1999. Morphological variability in a Me-diterranean, genetically homogeneous population of the European anchovy, Engraulisencrasicolus. – Fish. Res. 42: 229-243.
Watkinson, D.A. and Gillis, D.M. 2005. Stock disc-rimination of Lake Winnipeg walleye based on Fourier and wavelet description of scale outline signals. – Fish. Res. 72: 193-203.
Volume 3, Issue 4 - Serial Number 10
Autumn 2016
Pages 319-326

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026