Comparison the effect of movento, a chemical pesticide, with chitosan, a biologic pesticide, on female reproductive system in Balb/C mice

Kaboli Kafshgiri, Sakineh; Parivar, Kazem; Baharara, Javad; Hayati Roodbari, Nasim; Kerachian, Mohammad Amin

doi:10.21859/acadpub.nbr.3.4.279

Comparison the effect of movento, a chemical pesticide, with chitosan, a biologic pesticide, on female reproductive system in Balb/C mice

Authors

Sakineh Kaboli Kafshgiri

Kazem Parivar

Javad Baharara

Nasim Hayati Roodbari

Mohammad Amin Kerachian

https://doi.org/10.21859/acadpub.nbr.3.4.279

Abstract

Chemical pesticides possess toxic and destructive impacts on the reproductive system and disrupt endocrine function. In this experimental study, the effect of movento, as a chemical pesticide, was investigated in comparison with chitosan, a biological pesticide, on the female reproductive system in vivo. In this study, 56 mice were randomly dived into 7 groups including control and experimental groups treated with movento and chitosan. After a 21-day treatment, mice were killed and their ovaries and blood being collected. In addition, the samples were fixed and stained with H & E method. The results exhibited that treatment with 2.5 and 5 mg/kg chitosan had no significant effect on the number and diameter of primary, secondary and antral follicles, while these items were significantly reduced in 10 mg/kg ch-itosan group and all movento-treated groups as well. In addition, the level of sexual hormones, such as estradiol, FSH and LH, was decreased in 10 mg/kg chitosan group and all movento-treated groups in comparison with the control gr-oup. The findings showed that movento affected the sexual hormone levels, ovary and ovarian follicle structure and in-duced abnormality in female reproductive system, while chitosan, as a biological pesticide, should be used due to its minimum effects on female reproductive system

Keywords

ovarian

follicle

sexual hormone

20.1001.1.24236330.1395.3.4.1.1

Bretveld, R.W., Thomas, C.M.G., Scheepers, P.T.J., Ziel-huis, G.A. and Roeleveld, N. 2006. Pesticide exposure: the hormonal function of the female reproductive system disrupted? – Reprod. Bio. Endocrinol. Bio. Med. Cent-ral 4: 1-14.
Etchechury, F.M, Gramont, S., Sanchez, R., Romero, C., Enriquez, R., Cervantes, L., Olibarria, R., Millan, G. and Madri, A. 2015. Spirotetramat induces histological and biochemical changes in Wistar rats. – Rev. Toxicol. 30: 215-217.
Farr, S.L., Cooper, G.S., Cai, J., Savitz, D.A. and Sandler, D.P. 2004. Pesticide useand menstrual cycle characte-ristics among premenopausal women in the agricultural health study. – Am. J. Epidemiol. 160: 1194-1204.
Fisher, R. and Weiss, H.C. 2008. Spirotetramat (Movento): discovery, synthesis and physico-chemical properties. – Bayer CropScience J. 61: 127-140.
Gupta, R.K., Miller, K.P., Babus, J.K. and Flaws, J.A. 2006. Methoxychlor inhibits growth and induces atresia of antral Follicles through an oxidative stress pathway. –Toxicol. Sci. J. 93: 382-389.
Meeker, J.D., Ryan, L., Barr, D.B. and Hauser, R. 2006. Exposure to non-persistent insecticides and male repr-oductive hormones. – Epidemiol. J. Pubmed 17: 61-68.
Mnif, W., Hassine, A., Bouaziz, A., Bartegi, A. and Thom-as, O. 2011. Effect of endocrine disruptor pestisides. A Review. – Int. J. Environ. Res. Public Health (IJERPH) 8: 2265-2303.
Mokhtar, H.I., Abdel, H.A., Elmazoudy, R.H., Abdelw-ahab, W.M. and Saad, M.I. 2013. Effect of methomyl on fertility, embryotoxicity and physiological parameters in female rats. – J. App. Pharm. Sci. (JAPS) 3: 109-119.
Nauen, R., Reckmann, U., Thomzik, J. and Thielert, W. 2008. Biological profile of spirotetramat (movento) a new two way systemic (ambimobile) insecticide against sucking species. – Bayer CropScience J. 61: 245-277.
Ngoula, F., Watcho, P., Bouseko, T.S., Kenfack, A., Tcho-umboué, J. and Kamtchouing, P. 2007. Effects of prop-oxur on the reproductive system of male rats. – Afr. J. Rep. Health (AJRH) 11: 125-132.
Ngoula, F., Ngouateu, O.B, Kana, J.R, Defang, H.F, Watc-ho, P., Kamtchouing, P. and Tchoumboué, J. 2012. Re-productive and Developmental Toxicity of Insecticides. – Afr. J. Rep Health (AJRH) 10:429-457.
Saleh, A.M.T., Hijji, A.M., Elel, S.J. and Al Yacoub, A.N. 2014. Effect of carbamate insecticide, Lannate, on the gonads of mice. – J. Adv. Lab Res. Biol. (JALRB) 5: 140-145.
Shama, D., Sangha, G.K. and Khera, K.S. 2014. Triazo-phos induced oxidative stress in female albino rats. – Int. J. Adv. Res. (IJAR) 2:746-754.
Sangha, G.K, Kaur, K. and Khera, K.S. 2013. Cyprem-ethrine-induced pathological and biochemical changes in reproductive organs of female rats. – J. Environ. Biol. 34: 99-105.
Sharma, D., Sangha, G.K. and Khera, K.S. 2015. Triazophos-induced oxidative stress and histomorph-ological changes in ovary of female Wistar rats. – J. Pe- stic. Biochem. Physiol. 117: 9-18.

Sverdrup, L.E., Bjorge, C., Eklo, O.M., Grung, M., Kall-qvist, T., Klingen, I., Lag, M. and Ropstad, E. 2012. Ri-sk assessment of the insecticide Movento 100 SC with the active substance spirotetramat. – Bayer CropScience J. 13: 1-25.
Yanti, S.D, Nugroho, P.T, Aprisa, R. and Mulyana, E. 2009. The potential of chitosan as alternative biopesti-cide for postharvest Plants. – As. J. Food Ag-I nd 19: 241-248.
Yehia, M.A, El-Banna, S.G. and Okab, A.B. 2007. Diaz-inon toxicity affects histophysiological and biochemical parameters in rabbits. – Exp. Toxicol. Pathol. 59: 215-25.