The effect of cold temperature on oxidative damage and activity of oxidative enzymes in tea leaves from northern Iran

Sariri, Reyhaneh; Raeofi Masooleh, Adeleh; Bakhshi Khaniki, Gholam Reza

doi:10.21859/acadpub.nbr.2.4.298

The effect of cold temperature on oxidative damage and activity of oxidative enzymes in tea leaves from northern Iran

Authors

Reyhaneh Sariri ¹

Adeleh Raeofi Masooleh ²

Gholam Reza Bakhshi Khaniki ²

¹ Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran

² Department of Biology, Tehran Shargh Branch, Payam Nour University, Tehran, Iran

https://doi.org/10.21859/acadpub.nbr.2.4.298

Abstract

Tea was planted in Lahijan by Kashefalsataneh in 1930. The main concern about important commercial plants such as tea is the formation of ice crystals in low temperatures. This can damage the live cells leading to lowering the quality of the plant and eventually its death. Formation of reactive oxygen species (ROS) and oxidative stress is the result of various environmental stresses leading to freezing. Investigating the variations in any of these factors could help to understand the mechanism of freeze resistance in ever-green plants. The aim of the present research was to investigate lipid peroxidation, the presence of antifreeze protein and variations in the activity of some antioxidant enzymes, including superoxide dismutase (SOD), ascorbate peroxidize (APX) and catalyse (CAT) in tea leaves subjected to 20, 0, -2, -5 and -8°C in tea leaves from the north of Iran. The results showed formation of an antifreeze protein with MW of about 20 KD in response to cold stress. It was also found that the activity of SOD, APX and CAT increased in tea leaves due to cold stress. The activity of SOD increased down to -8°C. APX and CAT increased their activity down to -5°C. On the other hand, the lipid per oxidation factor, MDA, was also elevated in response to the cold stress.

Keywords

antifreeze protein

Ascorbate peroxidase

Cold stress

catalyse

lipid per oxidation superoxide dismutase

20.1001.1.24236330.1394.2.4.6.9

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