اثر تنش های خشکی و شوری بر تولید هورمون IAA در باکتری‌های جداسازی شده از خاک-های شور و شور-سدیمی

نویسندگان

گروه مهندسی علوم خاک، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

چکیده
این تحقیق به­ منظور ارزیابی توانایی تولید هورمونIndole acetic acid (IAA) به­ وسیلۀ باکتری­ های جداسازی شده از خاک­ های متأثر از خشکی و شوری اجرا شد. به­ همین منظور ضمن جداسازی 400 باکتری از خاک­ های با درجات مختلف قابلیت هدایت الکتریکی و نسبت سدیم تبادلی، تغییرات تولید IAA، اثر مقادیر مختلف تریپتوفان و تنش­ های خشکی و شوری بر تولید این هورمون تحت بررسی قرار گرفت. نتایج نشان داد که اثر تنش­ های خشکی و شوری بر تغییرات تولید IAA از نظر آماری معنی­ دار بود. گونۀ باکتری Arthrobacter siccitolerans (جداسازی شده از خاک­ های شور-سدیمی)، مولد هورمون IAA است و مقایسۀ میانگین داده­ ها نشان داد که این باکتری در وضعیت تنش خشکی به میزان 3/84 درصد نسبت به جدایۀ RS11، هورمون بیشتری تولید کرد. در­حالی­که جدایۀ RS11 در شرایط بدون تنش خشکی در مرحله اول غربالگری، بیشترین مقدار IAA ( µg.ml-141/10) را نسبت به سایر باکتری­ها تولید کرد. همچنین siccitolerans A. تا قابلیت الکتریکی برابر با 40 دسی­زیمنس بر متر و پتانسیل اسمزی 25- بار همچنان به تولید IAA ادامه داد. مقدار تولید هورمون IAA به­ ازای مصرف هر واحد تریپتوفان، در این باکتری برابر با 0/1 و در باکتری­ های دیگر، کمتر از 0/1 بود. با توجه به آزمون­های انجام شده، برتری نسبی باکتری یاد­شده محرز شد و برای انجام آزمون­های مزرعه­ای در تنش­ های موجود در خاک­ های متأثر از خشکی، شوری و شور- سدیمی پیشنهاد شد.

کلیدواژه‌ها


عنوان مقاله English

The effect of drought and salinity tensions on IAA production in isolated bacteria from saline and saline-sodic soils

نویسندگان English

Reza Soleimani
Hasan Tofighi
Hosseinali Alikhani
Department of Soil Sciences, College of Agriculture and Natural Resources, Tehran University, Karaj, Iran
چکیده English

This investigation was carried out to evaluate Indole acetic acid (IAA) production by isolated bacteria from drought and salinity affected soils. Four hundred bacteria were isolated from soils with different levels of electrical conductivity (EC) and sodium adsorption ratio (SAR). Then the ability of these bacteria to produce IAA and the effects of different amounts of tryptophan and drought-salinity tensions on IAA production were analyzed. Results indicated that the effects of drought and salinity tensions on variability of IAA production were statistically significant. One of the bacterial species isolated from saline-sodic soils, Arthrobacter siccitolerans, was IAA producer and data comparison indicated that under drought stress conditions it produced IAA 84.3% higher than isolate RS11. While isolated RS11 produced highest rate of IAA (10.42 µg.ml-1) under normal conditions. Also, A.siccitolerans continued to produce IAA until the EC was 40 dS.m-1 and OP (Osmosis pressure) was -20 bar. The amount of IAA production per unit of applied tryptophan in this bacterium was 0.1 and in others it was lesser. According to experiments, the relative preference of this bacterium was verified and proposed for field experiments in drought, salinity and salinity-sodicity affected soils.

کلیدواژه‌ها English

drought
electrical conductivity
IAA
sodium adsorption ratio
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  • تاریخ دریافت 18 خرداد 1405
  • تاریخ انتشار 18 خرداد 1405