Responses of grape (Vitis vinifera L.) antioxidant system to salinity

Authors

Urmia University

Abstract
Salinity is one of the important environmental factors that limit plant growth and product. Grapes are classified as salt sensitive plants. This paper attempts to evaluate the salinity effects on membrane lipid peroxidation, antioxidant components and antioxidative enzymes activity in four grape genotypes (Vitis vinifera L., Gharashani, LaaleBidaneh, Sachagh and Shahroodi) that commonly grow in the regions around Urmia Salt Lake. We came to the conclusion that malondialdehyde content and antioxidative enzymes activity increased significantly (P<0.05) in roots and leaves of all these genotypes. Gharashani and LaaleBidaneh genotypes showed higher antioxidative enzymes activity and lower membrane lipid peroxidation. Also, salinity had a significant effect on the accumulation of total phenolics content and phenylalanine ammonia lyase activity in all genotypes. Gharashani genotype showed the highest total phenols and PAL activity. There was a significant positive correlation among antioxidant enzymes activity, total phenolics content and PAL activity in leaves of all genotypes. It seems that Gharashani and LaaleBidaneh genotypes have a better antioxidant system compared with others and show higher efficiency for salinity tolerance.

Keywords


Abbaspour, N. 2008: A comparative study of Cltransport across the roots of two grapevine rootstocks, K 51-40 and Paulsen, differing in salt tolerance. – Ph.D thesis, University of Adelaide.
Asada, K. and Chen, G.X. 1989. Ascorbate peroxidase in Tea leaves: Occurrence of two isozymes and differences in their enzymatic and molecular properties. – Plant and Cell Physiology 30: 987-998.
Ashraf, M. and Fooland, M.R. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. – Environmental and Experimental Botany 59: 206-216.
Bonilla, E.P., Akoh, C.C., Sellappan, S. and Krewer, G. 2003. Phenolic content and antioxidant capacity of Muscadine grapes. –
Journal of Agricultural and Food Chemistry 51: 5497-5503.
Bor, M.F., Zdemir, Ü. and Turkan, I. 2003. The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. – Plant Science 164: 77-84.
De-Pinto, M.C., Tommasi, F. and De-Gara, L. 2002. Changes in antioxidant systems as part of the signaling pathway responsible for the programmed cell death activated by nitric oxide and reactive oxygen species in tobacco bright yellow 2 cells. – Plant Physiology 130: 698-708.
Dixon, R.A. and Paiva, N.L. 1995. Stressinduced phenylpropanoid metabolism. – Plant Cell 7: 1085-1097.
Garratt, L.H., Janagoudar, B.S., Low, K.C., Power, J.B. and Davey, M.R. 2002. Salinity tolerance and antioxidant status in cotton cultures. – Biol. Med. 33: 502-511.
Heath, R.L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts. – Archives of Biochemistry and Biophysics 125: 189-198.
Hernandez, J.A. and Almansa, M.S. 2002. Short-term effects of salt stress on antioxidant systems and leaf water relations of leaves. – Physiologia Plantarum 115: 251-257.
Hernandez, J.A., Jimenez, A., Mullineaux, P. and Sevilla, F. 2000. Tolerance of pea to long term salt stress is associated with induction of antioxidant defenses. – Plant, Cell and Environment 23: 853-862.
Hong, Z., Lakkineni, K., Zhang, Z. and Verma, D.P.S. 2000. Removal of feedback inhibition of D1-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. – Plant Physiology 122: 1129-1136.
Liang, Y.C., Chen, Q., Liu, Q., Zhang, W. and Ding, R. 2003. Exogenous silicon (Si) increases antioxidant enzyme activities and reduced lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). – Plant Physiology 160: 1157-1164.
Maehly, A.C. and Chance, B. 1959. The assay of Catalase and Peroxidase. In: ClickD. (Ed.): Methods of Biochemical Analysis. – Interscience, New York. 357-425.
Maas, E.V. and Hoffman, G.J. 1977. Salt crop tolerance , current assessment. – Journal of the Irrigation and Drainage Division 6: 115-134.
Shalata, A., Mittova, V., Volokita, M., Guy, M. and Tal, M. 2001. Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative Stress: The root antioxidant system. – Physiologia Plantarum 112: 487-494.
Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T. and Yabuta, Y. 2002. Regulation and function of ascorbate peroxidase isoenzymes. – Journal of Experimental Botany 53: 1305-1319.
Solecka, D. and Kacperska, A. 2003. Phenylpropanoid deficiency affects the course of plant acclimation to cold. – Physiologia Plantarum 119: 253-262.
Streb, P. and Feierabend, J. 1996. Oxidative stress response accompanying photo induction of catalase in NaCl-treated rye leaves. – Botanica Acta 109: 125-132.
Sudha, G. and Ravishankar, G.A. 2002. Involvement and interaction of various signaling compounds on the plant metabolic events during defense response, resistance to stress factors, formation of secondary metabolites and their molecular aspects. – Plant Cell, Tissue and Organ Culture 71: 181-212.
Walker, R.P., Torokfalvy, E., Scott, N.S. and Kriedemann, P.E. 1981. An analysis of photosynthetic response to salt treatment in Vitis vinifera. – Australian Journal Plant Physiology 8: 359-374.
Yahubyan, G., Gozmanova, M., Denev, I., Toneva, V. and Minkov, I. 2009. Prompt response of superoxide dismutase and peroxidase to dehydration and rehydration of the resurrection plant Haber learhodopensis. – Plant Growth and Regulation 57: 49-56.

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026