Nova Biologica Reperta

Histological effects of the smoke of Verbascum speciosum leaves on the healing of rats wounds

Authors

Mohammad Nebiuni
Shahrbanoo Oryan
Mohammad Ayobipour
Tayebeh Ramezani

Kharazmi University

https://doi.org/10.21859/acadpub.nbr.2.1.25
Abstract
The aim of this study was to assess the effect of the smoke of Verbascum speciocum leaves grown in 'Bouein Zahra' region, on the healing of skin lesions in Wistar rats. In this study in vivo conditions, 36 adult male Wistar rats were randomly divided into 3 groups. The operation day was considered as day zero and the procedure took 10 days. After the end of the treatment, i.e., on the 14th day, rats were killed by choloroform and samples were collected for histological analysis Masson trichrome and Vangason staining for collogen and hydroxyprolline amino acid OD assessment was applied. Studies in experimental groups exposed to the smoke of the leaves revealed that there was no increase in epidermis thickness, hair follicles diameter, skin, nor in the number and diameter of blood vessels as compared with control and sham groups. However, the amount of hair follicles was decreased. There was an increase in collogen diameter but this increase was not significant. But a significant increase was observed in OD of hydroxyl proline amino acid assessment (p<0.001) in experimental groups compared with sham and control groups. The results indicated that the use of smoke of V.speciocum leaves on the skin lesion had absolutely no increase on the wound healing ratios and its closure; it maximally could had very little effect on the proliferation as one step in the repair process, which needs further investigation.

Keywords

skin lesion
wound healing
Verbascum speciocum
leaf smoke
adult male wistar rat
AbdollahzadeIpek, S., Irem, T., EsraKupeli, A. and Hikmet, K. 2010. An ethno pharmaclogical study on Verbascum species: from conventional wound healing use to scientific verification. – J. of Ethnopharmacoloy 16: 56-70.
Bozkurt, T.E., Kahraman, C., Tatli, I.I. and Erdemlisahin, I. 2007. Free radical scavenging activity on Verbascum latisepalum extract. – J. of Bihat Kusadasi Turkey 45:26-29.
Brown, L.F., Yeo, K.T., Senger, D.R., Dvorak, H.F. and Vande Water, L. 1992. Expression of vascular permeability factor (vascular endothelial growth factor) by epidermal keratinocytes during wound healing. – J. Exp. Med. 176: 1375-1379.
Changizi, V., Nilforoush-Zadeh, M.A., Kakanezhadian, P. and Mardani, H. 2010. Histological study of open wound healing with a light cure instrument. – Iranian Journal of Medical Physics 3:9-14.
Fard, A., Zarifkar, A. and Dehghan, G.A. 2009. Effects of systemic administration of estradiol on the process of wound healing in excisional wound of diabetic rats. – Urmia Medical Journal 20: 26- 33.
Grinnell, F., Billingham, R. and Burgess, L. 1981. Distribution of fibronectin during wound healing in vivo. – J. Invest. Dermatol. 76: 181- 189.
Hanrahan, C. 2005. The Gale encyclopedia of alternative Medicine. – J. Frey Mullein 3: 345- 349.
Heidari, S.H., Parivar, K. and Yaghmai, P. 2009. In vitro study on the effect of Kombucha on the injured skin of adult mice NMRI strain. – J. North Azad University Developmental Biology 3: 1-6.
Houcair, M.M. and Phillips, T.G. 1997. What is new inclinical research in wound healing? – Clin. Dermatol. 15: 45-54.
Jimenez, C. and Riguere, R. 1994. Phenylethanoidglaycosides in plants structure and biological activting. – Natural Product Reports 11: 591-606.
Kurmari, M., Ambekar, M., Babu, S. and Kumar, N. 2010. Wound healing activity of aqueous extract of Crotalaria verrucosa in wistar albino rats. – Asian Pasific Journal of Tropical Medicine 3: 783-787.
Kupeli, E., Tatli, I.I., Akdemir, Z.S. and Yesilada, E. 2007. Biossay-guided isolation of anti-inflammatory & anti nociceptiv eglyco terpenoids from the flower of Verbascum lasianthum Boiss. ex Bentham. – J. of Ethnopharmacology 110: 444-450.
Lau, T.W., Lam, F.F.Y., Lau, K.M. and Chan, Y.W. 2009. Pharmacological investigation on the wound healing effect of Radix Rahmanniae in an animal model of diabetic foot ulcer. ― J. of Ethnopharmacology 123: 155-162.
Lee, M.J., Lee, O.H., Yoon, S.H., Lee, S.K., Chung, M.H., Park, Y.I., Sung, C.K., Choi, J.S. and Kim, K.W. 1998. In vitro angiogenic activity of Aloe veraon calf pulmonary artery endothelial (CPAE) cells. – Archives of Pharmacological Research 21:260-265.
Mirhaidar, H. 2005. Plant Sciences. – Nashre Farhange Eslami. 418-423 pp.
Nabiuni, M., Oryan, S.H., Ayyobipour, M., Bagheri, M. 2011. Histochemical study of Verbascumspeciocum extract's effects on the wound healing in rats. – J. of Cell & Tissue 2: 67- 75.
Norris, D.A., Clark, R.A.F., Swigart, L.M., Huff, J.C., Weston, W.L. and Howell, S.E. 1982. Fibronectin fragments are chemotactic for human peripheral blood monocytes. – J. Immunol. 129:16-1618.
Parvin, N., Validi, M., Banitalebi, M., Mobini, G.H., Ashrafi, K., Farrkhi, E., Rafieyan, M., Akbari, N., Safdari, F. and Rafie, L. 2010. Effect of medicinal smoking on some Nosocomial infection factor. – J. of Shahrekord University of Medical Sciences 12: 76-83.
Pesin Suntar, I., Kupeli Akkol, E., Yilmazer, D. and Baykal, T. 2010. Investigations on the in vivo wound healding potential of Hypericumper foratum. – J. of Ethnopharmalolagy 12: 468-477.
Reddy, S.J., Rao, R.P. and Reddy, S.M. 2002. Wound healing effects of Heliotropicum, Plumbago zeylanicum and Acalypha indica in rats. – Journal of Ethnopharmacology 79: 249- 251.

Ruggeri, Z.M. 2002. Platelets in atherothrombosis. – Nat. Med. 8: 1227-1234.
Salt, T.A., Tocker, J.A. and Adler, J.H. 1987. Dominance of 5-sterols in eight species of the Cactaceae. – Phytochemistry 26: 731-733.
Singh, M., Govindarajan, R., Nath, V., Rawat, S.K.A. and Mehrotra, S. 2006. Antimicrobial wound healing and antioxidant activity of Plagiochasma appendiculatum lehm et lind. – Journal of Ethnopharmacology 79: 249-251.
Tavakoli, R., Najafi Pour, H., Hadian, M.R., Aliyaei, G.H., Nabipour, F. and Talebian, S. 2004. Comparison of the effect of infrared (IR) and Phenytoin cream on skin wound healing in rat. – J. of Babol University of Medical Sciences 2: 7-11.
Tavakoli, R., Nabipour, F. and Najafipour, H. 2006. Effect of Betadine on wound healing in rat. – J. of Babol University of Medical Sciences 3: 7- 12.
Yaghmayei, P., Moshrefjavadi, F., Nilforooshzade, M.A. and Mardani, H. 2009. The effect of 2% alcohol green tea extract on healing process of open wound in male mice. – J. of Isfahan Medical School 96: 324-335.
Zareeyan, P., Zahiry, S.H., Ketabchy, F. and Rozmeh, S.H. 2007. Effects of gazangebin ointment on wound healing in rabbits. – J. of Mazandaran University of Medical Sciences 57: 1-8.
Zehila, A., Cigdem, K., Irem, T., EsraKupeli, A. and Ipek, S. 2010. Bioassay-guided isolation of anti-inflammatory, antinociceptive and wound healer glycosides from the flowers of Verbascum Mucronatum. – J. of Ethnopharmacology 136: 436-443.

  • Receive Date 08 June 2026
  • Publish Date 08 June 2026

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us