Effect of Low Density Lipoprotein-based semen extender supplementation with cysteinon on goat sperm quality after freezing

Ghorbanzadeh, Javad; Zeinoaldini, Saeed; Zhandi, Mahdi; Hussaini, Sayyed Mohammad Hadi

doi:10.22034/nbr.2026.11546

Effect of Low Density Lipoprotein-based semen extender supplementation with cysteinon on goat sperm quality after freezing

Document Type : Original Article

Authors

Javad Ghorbanzadeh

Saeed Zeinoaldini

Mahdi Zhandi

Sayyed Mohammad Hadi Hussaini

University of Tehran

https://doi.org/10.22034/nbr.2026.11546

Abstract

Objective: This study aimed to investigate the effect of adding cysteine to an Low Density Lipoprotein (LDL)-based extender on some quality parameters of goat sperm after freezing. Method: This study was conducted in a completely randomized design with four treatments, including: egg yolk-based extender without cysteine (EY), LDL-based extender containing zero (LDL-C0), five (LDL-C5), and 10 (LDL-C10) mM cysteine, and six replicates. Semen samples after dilution with the aforementioned extenders, were frozen. Results: After thawing, total and progressive motility, sperm plasma membrane integrity, functionality, and sperm morphology were evaluated. Total motility was higher in LDL-C5 compared to EY and LDL-C10 (P <0.05). Progressive motility in all treatments using LDL-based extender was significantly higher than EY. Other motility parameters were higher in LDL-C5 compared to EY (P <0.05). However, no significant differences were observed between the LDL-C0, LDL-C5, and LDL-C10, except for the VSL, which was significantly lower in LDL-C0 compared to LDL-C5 (P <0.05). Sperm plasma membrane integrity, functionality, and sperm morphology were higher in all treatments using LDL-based extender compared to EY (P <0.05), with the highest values observed in LDL-C5. Conclusions: Overall, the results showed that adding 5 mM cysteine to the LDL-based extender can improve some quality parameters of goat sperm and using semen from this group may enhance both in vitro and in vivo fertility.

Keywords

Antioxidants

Freesing

Goat

Quality

Sperm