Department of Biology, YI.C., Islamic Azad University, Tehran, Iran , s.m.maddah@gmail.com
Abstract: (616 Views)
Gastric cancer is one of the most common cancers, causing significant mortality worldwide. Nepeta species, known for their distinct aroma and taste, have garnered attention in the food industry. Given their phenolic compounds, this study aimed to evaluate the cytotoxic effects of Nepeta binaludensis and N. glomerulosa extracts on AGS gastric cancer cell lines and assess changes in BAX gene expression. Both species were collected from the Binalud Mountains in Khorasan, and their hydroalcoholic extracts were prepared via maceration. The amounts of caffeic acid and Rosmarinic acid were measured using HPLC. The extracts' cytotoxic effects were evaluated with the MTT assay, and apoptosis induction was assessed via DAPI staining. Changes in BAX gene expression were measured using real-time PCR. The IC50 values after 48 hours were 3.70 mg/mL for N. binaludensis extract and 6.92 mg/mL for N. glomerulosa extract. Apoptosis induction was observed under both treatments, but N. binaludensis extract, which contained higher Rosmarinic acid, significantly increased BAX expression compared to the control group. Thus, these extracts may inhibit gastric cancer cell growth, with N. binaludensis showing greater efficacy through BAX-mediated apoptosis induction compared to N. glomerulosa.
Type of Study: Original Article |
Subject:
Cell and Molecular Biology
Received: 2024/06/23 | Revised: 2025/04/30 | Accepted: 2025/04/16
Received: 2024/06/23 | Revised: 2025/04/30 | Accepted: 2025/04/16
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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