Volume 8, Issue 4 (1-2022)                   nbr 2022, 8(4): 242-252 | Back to browse issues page

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Meraji Masouleh Moghaddam M, Mashayekhi F, Zahiri Z, Eidi A. Analyzing the association of MMP-3 promoter polymorphism and its serum concentration with the outcome of in vitro fertilization and embryo transfer. nbr. 2022; 8 (4) :242-252
URL: http://nbr.khu.ac.ir/article-1-3480-en.html
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran , umistbiology20@gmail.com
Abstract:   (336 Views)
This study aimed to investigate the polymorphism of matrix metalloproteinase -3 (MMP-3) gene and its expression in the serum of infertile female patients received in vitro fertilization and embryo transfer (IVF-ET). To do so, 100 women with unsuccessful IVF-ET (IVF) and 100 women with successful IVF-ET procedure and clinical pregnancy (IVF+) were included. Genetic polymorphism and serum concentration of MMP3 were investigated by ARMS-PCR and ELISA, respectively. The results showed no significant association between MMP-3 gene polymorphism and IVF-ET outcome among the two groups studied. However, a significant decrease in the concentration of MMP-3 serum in the IVF group was observed in comparison with the IVF+ group (P=0.000002). Moreover, we showed that the serum MMP-3 levels in CC, AC and AA genotypes in the IVF group were 33, 65.33 and 86 ng/ml, respectively. In conclusion, while there is no significant difference between MMP-3 promoter polymorphism and IVF-ET outcome between the IVF+ and IVF- groups, a significant decrease in MMP-3 serum levels in IVF- group was seen as compared with the IVF+ group. It could be also suggested that the CC genotype is associated with a decreased level of MMP-3 serum concentration and may be associated with IVF-ET failure.
 

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Type of Study: Original Article | Subject: Cell and Molecular Biology
Received: 2021/08/13 | Revised: 2022/01/18 | Accepted: 2021/10/19 | Published: 2022/01/17 | ePublished: 2022/01/17

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