Volume 5, Issue 4 (3-2019)
nbr 2019, 5(4): 458-465 |
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Saboora A, Amiri Rad M, Asgarani E, Radjabian T. Comparison study of three methods for genomic DNA extraction from fresh and herbarium leaf specimens of Achillea wilhelmsii C. Koch. nbr 2019; 5 (4) :458-465
URL: http://nbr.khu.ac.ir/article-1-2974-en.html
URL: http://nbr.khu.ac.ir/article-1-2974-en.html
alzahra university , saboora@alzahra.ac.ir
Abstract: (6346 Views)
DNA extraction from plant tissues often causes most problems. For example, unsuccessful removal secondary metabolites during extraction, such as phenolic compounds in aromatic and medicinal plants, cause to some mistakes in result of molecular experiments by using of the extracted DNA. Achillea wilhelmsii is a medicinal plant belong to Asteraceae family and native to Iran, there is little information about genomic data in this plant. Therefore, optimizing of the DNA extraction methods for obtaining suitable quality and quantity yield is necessary. In this study, two traditional DNA extraction methods (using fresh and herbarium leaf samples) and commercial DNA kit MAGNANTM in Yarrow have been compared. Results showed that DNA extracted from fresh leaves of yarrow according to Khanuja et al. (1999) was better than the other methods which mentioned in this research, because of the increased amount of extracted DNA and reduced harmful compounds such as RNA, polysaccharides, protein and secondary metabolites. To evaluate the quality of extracted DNA from herbarium specimens showed that, in spite of the high yielding DNA (10-50 fold ratio to the other methods), quality of this extract was low on agarose gel because of smear and broken down the molecules. It can be considered as a limitation of the extract in molecular experiments. Procedure of MAGNANTM DNA kit was appropriate for reducing time and cost of the extraction as well as low contamination to protein and RNA, but the process needs some modifications for yarrow to increase the amount of extracted DNA.
Keywords: Achillea wilhelmsii, genomic DNA extraction, herbarium samples, magnetic nanoparticle, polyphenols
Type of Study: Original Article |
Subject:
Plant Biology
Received: 2017/10/2 | Revised: 2021/06/1 | Accepted: 2018/05/28 | Published: 2019/03/18 | ePublished: 2019/03/18
Received: 2017/10/2 | Revised: 2021/06/1 | Accepted: 2018/05/28 | Published: 2019/03/18 | ePublished: 2019/03/18
References
1. Alaey, M., Nader, R., Khalighi, A., Vezvaeoi, A. and Salami, A.R. 2007. Study on effective components on the quantity and quality of genomic DNA extracted from Iran cyclamen species. – Pajouhesh & Sazandegi 73: 11-16.
2. Asgary, S., Naderi, G.H., Sarrafzadegan, N., Mohammadifard, N., Mostafavi, S. and Vakili, R. 2000. Anthypertensive and antihyperlipidemic effect of Achillea wilhelmsii. – Drugs Exp. Clin. Res. 26: 89-93.
3. Bushra, C., Afshan, Y., Tayyab, H. and Riazuddin, S. 1999. Mini-scale genomic DNA extraction from cotton. – Plant Mol. Biol. Rep. 17: 1-7. [DOI:10.1023/A:1017213630972]
4. Cheng, Y.J., Guo, W.W., Yi, H.L., Pang, X.M. and Deng, X. 2003. An efficient protocol for genomic DNA extraction from Citrus species. – Plant Mol. Biol. Rep. 21: 177-186. [DOI:10.1007/BF02774246]
5. Costa, C.M. and Roberts, R.P. 2014. Techniques for improving the quality and quantity of DNA extracted from herbarium specimens. – Phytoneuron 48: 1-8.
6. Cota-Sánchez, J.H., Remarchuk, K. and Ubayasena, K. 2006. Ready to use DNA extracted with a CTAB method adapted forherbarium specimens and mucilaginous plant tissue. – Plant Mol. Biol. Rep. 24: 161-167. [DOI:10.1007/BF02914055]
7. Del Serrone, P., Attorri, L. and Palazzino, G. 2007. Easy DNA extraction for rapid detection of Panax ginseng C.A. Meyer in commercial ginseng products. – Nat. Prod. Res. 21: 1099-1103. [DOI:10.1080/14786410600879789]
8. Dellaporta, S.L., Wood, J. and Hicks, J.B. 1983. A plant DNA minipreparation: Version II. – Plant Mol. Biol. Rep. 4: 19-21. [DOI:10.1007/BF02712670]
9. Desjardins, P. and Conklin, D. 2010. NanoDrop Microvolume Quantitation of Nucleic Acids.- J. Vis. Exp. 45: 1-4. [DOI:10.3791/2565]
10. Drábková, L.Z. 2014. DNA extraction from herbarium specimens. – Methods Mol. Biol. 1115: 69-84. [DOI:10.1007/978-1-62703-767-9_4]
11. Gaudeul M, and Rouhan G. 2013. A plea for modern botanical collections to include DNA-friendly material. – Trends Plant Sci. 18:184-5. [DOI:10.1016/j.tplants.2012.12.006]
12. Kadkhodaie, A. 2003. A simple and low cost method for extraction of nucleic acids from plants containing polysaccharides and polyphenols: Optimizing the DNA extraction method in almonds. – Proceedings of the Third Iranian Congress of Biotechnology 417-419.
13. Khanuja, S.P.S., Shasany, A.K., Darokar, M.P. and Kumar, S. 1999. Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils. – Plant Mol. Biol. Rep. 17: 1-7. [DOI:10.1023/A:1007528101452]
14. Komatsuda, T., Nakamura, I., Takaiwa, F. and Oka, S. 1998. Rapid, small scale DNA preparation from barley leaves. – Genome 41: 680-685. [DOI:10.1139/g98-069]
15. Lakshmi, T., Geetha, R.V., Roy, A. and Kumar, S.A. 2011. Yarrow (Achillea millefolium LINN.) A herbal medicinal plant with broad therapeutic use – a review. – Int. J. Pharm. Sci. Rev. Res. 9: 136-141.
16. Lickfeldt, D.W., Hofmann, N.E., Jones, J.D., Hamblin, A.M. and Voigt, T.B. 2002. Comparing three DNA extraction procedures for cost, efficiency, and DNA yield. – Hort. Sci. 37: 822-825.
17. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. – Physiol. Plant. 15: 473-497 [DOI:10.1111/j.1399-3054.1962.tb08052.x]
18. Murry, M. and Thompson, W.F. 1984. Rapid isolation of high molecular weight plant DNA. – Nucleic Acid Res. 8: 4321-4325. [DOI:10.1093/nar/8.19.4321]
19. Pirttila, A.M., Hirsikorpi, M., Kamarainen, T., Jaakola, L. and Hohtola, A. 2001. DNA isolation methods for medicinal and aromatic plants. – Plant Mol. Biol. Rep. 19: 273a-273f. [DOI:10.1007/BF02772901]
20. Porebski, S.L., Baily, G. and Baum, B.R. 1997. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. – Plant Mol. Biol. Rep. 15: 8-15. [DOI:10.1007/BF02772108]
21. Rahim Malek, M. 2011. Investigating and comparing different methods of DNA extraction in medicinal plants and aromatic plants. – Herbal remedies. 1: 1-5.
22. Rahnama, H., Sattarzadeh, A., Kazemi, F., Ahmadi, N., Sanjarian, F. and Zand, Z. 2016. Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction. – Anal. Biochem. 513: 68-76 [DOI:10.1016/j.ab.2016.08.029]
23. Sahare, P. and Srinivasu, T. 2012. Method for the isolation of genomic DNA from medicinal plants producing large amount of secondary metabolites. – IJES. 1: 37-39.
24. Sameer, S.A., Ul Rehman S., Banday, M.Z., Syeed, N., Pandit, A.A., Nanda, M.S. and Siddiqi, M.A. 2009. Comparison of the Different Methods of DNA Extraction from Snap-frozen Tumor Tissues: Our Experience. – IJBB. 5: 43-50.
25. Varadarajan, G.S. and Prakash, C.S. 1991. A rapid and efficient method for the extraction of total DNA from the sweet potato and its related species. – Plant Mol. Biol. Rep. 9: 6-12. [DOI:10.1007/BF02669284]
26. Viana, J.P.G., Borges, A.N.C., Lopes, A.C.A., Gomes, R.L.F., Britto, F.B., Lima, P.S.C. and Valente, S.E.S. 2015. Comparison of eight methods of genomic DNA extraction from babassu. – Genet. Mol. Res. 14: 18003-18008. [DOI:10.4238/2015.December.22.26]
27. Zabeti, S.M., Ismaili, A., Madah-Arefi, H., Nazarian-Firouzabadi, F. and Mojiri, F. 2014. Separation of secondary metabolites in DNA extraction process of Thymus and analysis of them by GC mass. – J. Mol. Cell. Res. (Iran. J. Biol.) 27: 66-74.
28. Zamani, Z., Sarkhoush, A., Fatahi Moghadam, M.R. and Ebadi, A. 2005. Comparison of Six Genomic DNA extraction from pomegranate (Punica granatum L.). – Iran. J. Hort. Sci. Technol. 6: 99-110.
29. Zhoua, L.G. and Wu, J.U. 2006. Development and application of medicinal plant tissue cultures for production of drugs and herbal medicinals in China. – Nat. Prod. Rep. 2: 789-810. [DOI:10.1039/b610767b]
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