Volume 6, Issue 4 (1-2020)                   nbr 2020, 6(4): 424-434 | Back to browse issues page


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Derikvand F, Bazgir E, Darvishnia M, Mirzaei Najjafgholi H. Fluctuation in some enzymes related to antioxidant defense system in common bean against Xanthomonas axonopodis pv. Phaseoli. nbr. 2020; 6 (4) :424-434
URL: http://nbr.khu.ac.ir/article-1-2973-en.html
University of Lorestan , Fatemehderikvand93@gmail.com
Abstract:   (706 Views)
Antioxidant enzymes play an important role in plant defense against pathogenic agents. Following the identification of the pathogen, plants produce active oxygen species (ROS) as one of their first defense responses. To maintain the balance of ROS levels and prevent their harmful effects, plants produce antioxidant peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and superoxide dismutase (SOD) enzymes. In the present study, the resistance of bean plants cultivars, namely Sadri, Paak, Darakhshan and Dorsa, to Xanthomonas axonopodis pv. phaseoli (Xap) were studied in greenhouse conditions. The catalase, peroxidase and ascorbate peroxidase enzyme activities were studied in healthy and Xap-infected bean cultivars Sadri and Derakhshan at 0, 24, 48, 72 hours and 20 days post inoculation by a completely randomized design with 5 treatments and 4 replications. The result showed that disease symptoms appeared in all tested cultivars. Derakhshan and Sadri cultivars, with 58.33 and 80.56 percentages of infected plants 20 days after inoculation, showed the least and highest infection rates, respectively. The highest catalase and peroxidase activities were recorded 24 and 48h post inoculation. These records reduced 48 and 72 hours post inoculation, respectively. The activities of these two enzymes in the susceptible cultivar were less than those in the semi-resistant one. The chlorophyll a and chlorophyll b contents of Xap-infected plants reduced significantly. The total chlorophyll content of uninfected Sadri and Darakhshan cultivars were 2.93 and 3.23 µg/g, respectively, which reduced to 1.96 and 2.14 µg/g of leaf tissue in infected plants, respectively. Based on these results, it is suggested that the Derakhshan cultivar should be planted in disease-susceptible regions as the semi-resistant cultivar.
 
 
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Type of Study: Original Article | Subject: Plant Biology
Received: 2017/09/30 | Revised: 2020/02/24 | Accepted: 2018/11/13 | Published: 2020/01/8 | ePublished: 2020/01/8

References
1. Aebi, H. 1984. Catalase in vitro. - Meth. Enzymol. 105: 121-126.‌ [DOI:10.1016/S0076-6879(84)05016-3]
2. Agrios, G.N. 2005. Plant Pathology (5th ed.) 5th. Elsevier Acad. Press. - Burlington. Mass, EU. 36: 31-45.
3. Akhavan, A., Bahar, M., Askarian, H., Lak, M.R., Nazemi, A. and Zamani, Z. 2013. Bean commonbacterial blight: pathogen epiphytic life and effect of irrigation practices. - Springer Plus 2: 41-49.‌ [DOI:10.1186/2193-1801-2-41]
4. Allen, R.D. 2008. Dissection of oxidative stress tolerance using transgenic plants. - Plant Physiol. 107: 1049-1054. [DOI:10.1104/pp.107.4.1049]
5. Angeles-Ramos, R., Vidaver, A.K. and Flynn, P. 1991. Characterization of epiphytic Xanthomonas campestris pv. phaseoli and pectolytic Xanthomonads recovered from symptomless weeds in the Dominican Republic. - Phytopath. 81: 677-681.‌ [DOI:10.1094/Phyto-81-677]
6. Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. - Plant Physiol. 24: 1.‌ [DOI:10.1104/pp.24.1.1]
7. Asada, K. 1992. Ascorbate peroxidase-a hydrogen peroxide‐scavenging enzyme in plants. - Physiol. Plantarum. 85: 235-241.‌ [DOI:10.1034/j.1399-3054.1992.850216.x]
8. Blokhina, O., Virolainen, E. and Fagerstedt, K. V. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. - Ann. Bot. 91: 179-194.‌ [DOI:10.1093/aob/mcf118]
9. Bolwell, G.P. and Daudi, A. 2009. Reactive oxygen species in plant-pathogen interactions. In reactive oxygen species in plant signalling. - Springer, Berlin, Heidelberg.‌ pp: 113-133. [DOI:10.1007/978-3-642-00390-5_7]
10. Bolwell, G.P., Bindschedler, L.V., Blee, K.A., Butt, V.S., Davies, D.R., Gardner, S.L. and Minibayeva, F. 2002. The apoplastic oxidative burst in response to biotic stress in plants: a three‐component system. - J. Exp. Bot. 53: 1367-1376.‌ [DOI:10.1093/jxb/53.372.1367]
11. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254.‌ [DOI:10.1016/0003-2697(76)90527-3]
12. Cavalcanti, F.R., Resende, M.L. V., Lima, J.P.M.S., Silveira, J.A.G. and Oliveira, J.T.A. 2006. Activities of antioxidant enzymes and photosynthetic responses in tomato pre-treated by plant activators and inoculated by Xanthomonas vesicatoria. - Physiol. Mol. Plant Pathol. 68: 198-208.‌ [DOI:10.1016/j.pmpp.2006.11.001]
13. Chandrashekar, S. and Umesha, S. 2012. Induction of antioxidant enzymes associated with bacterial spot pathogenesis in tomato. - Int. J. Food Agric. Vet. Sci. 2: 22-34.
14. Chatterton, S., Balasubramanian, P.M., Erickson, R.S., Hou, A., McLaren, D.L., Henriquez, M.A. and Conner, R.L. 2016. Identification of bacterial pathogens and races of Pseudomonas syringae pv. phaseolicola from dry bean fields in Western Canada. - Can. J. Plant Pathol. 38: 41-54.‌ [DOI:10.1080/07060661.2016.1141800]
15. Chithrashree, C. and Srinivas, C. 2012. Role of antioxidant scavenging enzymes and extracellular polysaccharide in pathogenicity of rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae. - African J. Biotech. 11: 13186-13193.‌
16. Corrales, M.A.P. and Van Schoonhoven, A. 1987. Standard system for the evaluation of bean germplasm. - CIAT‌ 23: 95-106.
17. Darsonval, A., Darrasse, A., Meyer, D., Demarty, M., Durand, K., Bureau, C. and Jacques, M.A. 2008. The type III secretion system of Xanthomonas fuscans subsp. fuscans is involved in the phyllosphere colonization process and in transmission to seeds of susceptible beans. - Appl. Environ. Microbiol. 74: 2669-2678.‌ [DOI:10.1128/AEM.02906-07]
18. Dhanya, M.K. and Mary, C.A. 2007. Management of bacterial blight of Anthurium (Anthurium andreanum Linden.) using ecofriendly materials. - J. Trop. Agric. 44: 74-75.‌
19. Djébali, N., Mhadhbi, H., Lafitte, C., Dumas, B., Esquerré-Tugayé, M.T., Aouani, M.E. and Jacquet, C. 2011. Hydrogen peroxide scavenging mechanisms are components of Medicago truncatula partial resistance to Aphanomyces euteiches. - Eur. J. Plant Pathol. 131: 559. [DOI:10.1007/s10658-011-9831-1]
20. Dursun, A., Figen Donmez, M. and Şahin, F. 2002. Identification of resistance to common bacterial blight disease on bean genotypes grown in Turkey. - Eur. J. Plant Pathol. 108: 811-813.‌ [DOI:10.1023/A:1020828130498]
21. Ebadzadeh, H.R., Ahmadi, K., Mohamadinia, SH., Abbastaghani, R., Abasi, M. and Yari, SH. 2017. Agriculture economic aspects Iran statistics. Agriculture Iran statistics. 403p.
22. Farahani, A.S. and Taghavi, M. 2016. Changes of antioxidant enzymes of mung bean [Vigna radiata (L.) R. Wilczek] in response to host and non-host bacterial pathogens. - J. Plant Pro. Res. 56: 95-99.‌ [DOI:10.1515/jppr-2016-0016]
23. Ferreira, C.F., Pereira, M.G., Dos Santos, A.D.S., Rodrigues, R., Bressan-Smith, R. E., PioViana, A. and Daher, R.F. 2003. Resistance to common bacterial blight in Phaseolus vulgaris L. reco mbinant inbred lines under natural infection of Xanthomonas axonopodis pv. phaseoli. - Euphytica 134: 43-46.‌
24. Gilbertson, R.L. and Maxwell, D.P. 1992. Common bacterial blight of bean. In Plant diseases of international importance. Vol II: 18-39. Edited by: Chaube, H.S., Kumar, J., Mukhopadhyay, A.N. and Singh, U.S. Prentice Hall, Englewood Cliffs, New Jersey.
25. Halliwell, B. 2006. Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. - Plant Physiol. 141: 312-322.‌ [DOI:10.1104/pp.106.077073]
26. Itako, A.T., Tolentino Júnior, J.B., Júnior, S., Soman, J.M. and Maringoni, A.C. 2015. Chemical products induce resistance to Xanthomonas perforans in tomato. - Braz. J. Microbiol. 46: 701-706.‌ [DOI:10.1590/S1517-838246320140177]
27. Karavina, C., Mandumbu, R., Parwada, C. and Zivenge, E. 2011. Epiphytic survival of Xanthomonas axonopodis pv. phaseoli (EF Sm). - J. Anim. Plant Sci. 9: 1161-1168.‌
28. Kumar, N., Ebel, R.C., and Roberts, P.D. 2011. Superoxide dismutase activity in kumquat leaves infected with Xanthomonas axonopodis pv. citri. - J. Hortic. Sci. Biotechnol. 86: 62-68.‌
29. Lak, M.R., Shamsbakhsh, M. and Bahar, M. 2002. Identification of the bacterial agent of bean leaf and pod blight in Markazi province. - JWSS-Isfahan University of Technology. 6: 231-243.‌
30. Mallick, N., and Mohn, F.H. 2000. Reactive oxygen species: response of algal cells. - Plant Physiol. 157: 183-193.‌ [DOI:10.1016/S0176-1617(00)80189-3]
31. Mirzaei Najafgholi, H., Tarighi, S., Golmohamadi, M. and Taheri, P. 2017. Antioxidant response of Mexican lime to Xanthomonas citri subsp. citri. - Agric. Biotechnol. 8: 9-17.
32. Pan, Y., Wu, L.J. and Yu, Z.L. 2006. Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). - Plant Growth Regul. 49: 157-165.‌ [DOI:10.1007/s10725-006-9101-y]
33. Ranieri, A., Castagna, A., Pacini, J., Baldan, B., Sodi, A.M. and Soldatini, G.F. 2003. Early production and scavenging of hydrogen peroxide in the apoplast of sunflower plants exposed to ozone. - J. Exp. Bot. 54: 2529-2540.‌ [DOI:10.1093/jxb/erg270]
34. Richards, G. and Roberts, P. 2016. Centre for Agriculture and Biosciences International (CABI). Review of Xanthomonas axonopodis pv. phaseoli (bean blight). http://www.cabi.org/isc/datasheet/56962.
35. Saettler, A.W. 1989. Assessment of yield loss caused by common blight of beans in Uganda. - Annu. Rep. Bean Improv. Coop. 35:113-114.‌
36. Terán, H., Lema, M., Webster, D. and Singh, S.P. 2009. 75 years of breeding pinto bean for resistance to diseases in the United States. - Euphytica 167: 341-351.‌ [DOI:10.1007/s10681-009-9892-9]
37. Todorović, B., Milijašević, S., Rekanović, E., Potočnik, I. and Stepanović, M. 2008. Susceptibility of bean genotypes to Xanthomonas campestris pv. phaseoli in greenhouse conditions. - Pesticidi i Fitomedicina 23: 167-173. [DOI:10.2298/PIF0803167T]
38. Venacker, H., Carver, T.L.W. and Foyer, C.H. 1998. Pathogen induced changes in the antioxidant status of the apoplast in barley leaves. - Plant Physiol. 117: 1103-1114. [DOI:10.1104/pp.117.3.1103]

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